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Product Info Summary
| SKU: | A04644 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-NDFIP1 Antibody
SKU/Catalog Number
A04644
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-NDFIP1 Antibody (Catalog # A04644). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
NDFIP1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-NDFIP1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04644)
Host
Rabbit
Contents
NDFIP1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
NDFIP1 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human NDFIP1. The immunogen is located within the first 50 amino acids of NDFIP1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
NDFIP1 antibody is predicted to not cross-react with NDFIP2.
Reactive Species
A04644 is reactive to NDFIP1 in Human, Mouse
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
24899 MW
Background of Ndfip1
The NEDD4 family-interacting protein 1 (NDFIP1) belongs to a small group of evolutionarily conserved proteins with three transmembrane domains and is an integral Golgi membrane protein. It is a potential target for ubiquitination by the Nedd4 family of proteins. NDFIP1 is strongly expressed in surviving neurons following acute cortical brain injury, and overexpression in cultured cortical neurons increased survival following growth factor starvation, suggesting that NDFIP1 may play a role in neuronal survival. NDFIP1 and the related protein NDFIP2 are thought to interact with and regulate multiple components of the EGF and PTEN/Akt signaling pathways. Recent studies suggest that NDFIP1 may also play a role in Th17 differentiation by limiting the production of proinflammatory cytokines.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04644 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
NDFIP1 antibody can be used for detection of NDFIP1 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of NDFIP1 in PC-3 cell lysate with NDFIP1 antibody at (A) 0.5 and (B) 1 μg/mL.
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Immunohistochemistry of NDFIP1 in human lung tissue with NDFIP1 antibody at 5 μg/ml.
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Immunofluorescence of NDFIP1 in human lung tissue with NDFIP1 antibody at 20 μg/ml.
Protein Target Info & Infographic
Gene/Protein Information For NDFIP1 (Source: Uniprot.org, NCBI)
Gene Name
NDFIP1
Full Name
NEDD4 family-interacting protein 1
Weight
24899 MW
Alternative Names
N4WBP5, N4WBP5, PSEC0192, PSEC0223, NEDD4 family-interacting protein 1, Breast cancer-associated protein SGA-1M Ndfip1|0610010M22Rik, N4wbp5|Nedd4 family interacting protein 1|NEDD4 family-interacting protein 1|NEDD4 WW domain-binding protein 5|Nedd4 WW binding protein 5
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NDFIP1, check out the NDFIP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NDFIP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NDFIP1 Antibody (A04644)
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