Anti-Napsin A (Lung Adenocarcinoma Marker) NAPSA Monoclonal Antibody

Product Name

Anti-Napsin A (Lung Adenocarcinoma Marker) NAPSA Monoclonal Antibody

View all Napsin A Antibodies

SKU/Catalog Number

M05685-1

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-Napsin A (Lung Adenocarcinoma Marker) NAPSA Monoclonal Antibody (Catalog # M05685-1). Tested in Flow Cytometry, IF, WB, IHC applications. This antibody reacts with Human.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-Napsin A (Lung Adenocarcinoma Marker) NAPSA Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05685-1)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: NAPSA/1238 + NAPSA/1239

Isotype

IgG, kappa

Immunogen

Recombinant human Napsin-A protein fragment (aa189-299) (exact sequence is proprietary)

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M05685-1 is reactive to NAPSA in Human

Reconstitution

Reconstitute with distilled water.

Observed Molecular Weight

68 kDa

Calculated molecular weight

45387 MW

Background of Napsin A

Napsin is a pepsin-like aspartic proteinase connected with maturation of surfactant protein B.There are two closely related napsins, napsin A and napsin B. Napsin A is expressed as a single chain protein. Immunohistochemical studies revealed high expression levels of napsin A in human lung and kidney but low expression in spleen. Napsin A is expressed in type II pneumocytes and in adenocarcinomas of lung. The high specificity expression of napsin A in adenocarcinomas of lung is useful to distinguish primary lung adenocarcinomas from adenocarcinomas of other organs.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M05685-1 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml for 60 minutes at RT)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

Gene/Protein Information For NAPSA (Source: Uniprot.org, NCBI)

Alternative Names

Napsin-A;3.4.23.-;Aspartyl protease 4;ASP4;Asp 4;Napsin-1;TA01/TA02;NAPSA;NAP1, NAPA; NAPSA KAP, Kdap, NAP1, NAPA, SNAPA napsin A aspartic peptidase napsin-A|ASP4|TA01/TA02|asp 4|aspartyl protease 4|kidney-derived aspartic protease-like protein|napsin-1|pronapsin A

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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