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Product Info Summary
| SKU: | EK1507 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma (heparin, EDTA) , saliva, urine and human milk. |
Product info
Product Name
Human BCHE/Butyrylcholinesterase ELISA Kit PicoKine®
View all Butyrylcholinesterase/BCHE ELISA kits
SKU/Catalog Number
EK1507
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human BCHE/Butyrylcholinesterase ELISA Kit PicoKine® (96 Tests). Quantitate Human BCHE in cell culture supernatants, serum, plasma (heparin, EDTA) , saliva, urine and human milk. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human BCHE/Butyrylcholinesterase ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1507)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: E29-L602
Sensitivity
<10 pg/ml
Assay Range
625 pg/ml - 40,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1507 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1507 is reactive to BCHE in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA) , saliva, urine and human milk.
Application Guarantee
EK1507 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Butyrylcholinesterase/BCHE
Butyrylcholinesterase is a nonspecific cholinesterase enzyme that in humans is encoded by the BCHE gene. This gene is mapped to 3q26. It is a member of the type-B carboxylesterase/lipase family of proteins. The encoded enzyme exhibits broad substrate specificity and is involved in the detoxification of poisons including organophosphate nerve agents and pesticides, and the metabolism of drugs including cocaine, heroin and aspirin. Humans homozygous for certain mutations in this gene exhibit prolonged apnea after administration of the muscle relaxant succinylcholine.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1507-CAP | Anti-Human BCHE Pre-coated 96-well strip microplate | 1 |
| EK1507-ST | Human BCHE Standard | 2 vials, 40 ng/tube |
| EK1507-DA | Human BCHE Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 | 40000 |
| O.D. | 0.015 | 0.076 | 0.144 | 0.250 | 0.466 | 0.985 | 1.607 | 2.036 |
Data Example Images
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Human BCHE PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Butyrylcholinesterase/BCHE, Dilution ratio of 1:1000, concentration in serum and plasma is 1833-8048 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human BCHE/Butyrylcholinesterase ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 1205 | 4078 | 24826 | 1297 | 4376 | 24447 |
| Standard deviation | 66.27 | 322.16 | 1613.69 | 80.41 | 380.71 | 1662.39 |
| CV (%) | 5.5% | 7.9% | 6.5% | 6.2% | 8.7% | 6.8% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Butyrylcholinesterase/BCHE in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 1205 | 1122 | 1225 | 1228 | 1195 | 43.06 | 3.6% |
| Sample 2 | 4078 | 4285 | 4341 | 4276 | 4245 | 99.58 | 2.3% |
| Sample 3 | 24826 | 24993 | 26084 | 24591 | 25123 | 572.63 | 2.2% |
Protein Target Info & Infographic
Gene/Protein Information For BCHE (Source: Uniprot.Org, NCBI)
Gene Name
BCHE
Full Name
Cholinesterase
Weight
68418 MW
Superfamily
type-B carboxylesterase/lipase family
Alternative Names
Cholinesterase;3.1.1.8;Acylcholine acylhydrolase;Butyrylcholine esterase;Choline esterase II;Pseudocholinesterase;BCHE;CHE1; BCHE BCHED, CHE1, CHE2, E1 butyrylcholinesterase cholinesterase|acylcholine acylhydrolase|butyrylcholine esterase|choline esterase II|cholinesterase (serum) 2|cholinesterase 1|pseudocholinesterase
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on BCHE, check out the BCHE Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for BCHE: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human BCHE/Butyrylcholinesterase ELISA Kit PicoKine® (EK1507)
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No publications found for EK1507
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Customer Reviews
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Customer Q&As
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10 Customer Q&As for Human BCHE/Butyrylcholinesterase ELISA Kit PicoKine®
Question
What is the kind and concentration of acid contained in Stop Solution of EK1507?
Verified customer
Asked: 2021-11-12
Answer
The Stop solution for Human BCHE/Butyrylcholinesterase ELISA Kit PicoKine® (EK1507) contains 2% Sulfuric acid.
Boster Scientific Support
Answered: 2021-11-12
Question
Does any component in EK1507 contain sodium azide or thimersol?
Verified customer
Asked: 2021-11-10
Answer
The Human BCHE/Butyrylcholinesterase ELISA Kit PicoKine® (EK1507) doesn't contain any sodium azide or thimersol.
Boster Scientific Support
Answered: 2021-11-10
Question
Q: What is the optimal O.D. value for BCHE ELISA kit? I used your BCHE ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain BCHE even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2020-11-15
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. in your example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this BCHE ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of BCHE that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2020-11-15
Question
Q: Can BCHE ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2020-08-23
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2020-08-23
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2020-03-13
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2020-03-13
Question
How many samples can be tested in one Human BCHE/Butyrylcholinesterase ELISA Kit PicoKine™ (EK1507) unit?
Verified customer
Asked: 2019-10-13
Answer
The amount of samples you can run depend on a few factors. In the most common ELISA set up, you will use two strips for standards, and 10 strips for samples using duplicates, which let you run up to 40 samples per kit.
Boster Scientific Support
Answered: 2019-10-13
Question
Q: can I use citrate plasma as samples in Human BCHE Picokine® ELISA Kit (Catalog # EK1507)?
G. Thomas
Verified customer
Asked: 2019-04-18
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of BCHE causing disruption of its protein structure. BCHE may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the BCHE ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2019-04-18
Question
Q: which procedure should I follow in order to thaw whole blood sample for BCHE ELISA after freezing?
Z. Baker
Verified customer
Asked: 2018-09-17
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test BCHE for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2018-09-17
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification BCHE using the EK1507 Human BCHE Picokine® ELISA Kit?
B. Garcia
Verified customer
Asked: 2018-05-31
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples in order to prevent protein degradation.
Boster Scientific Support
Answered: 2018-05-31
Question
Q: can you suggest the dilution ratio of serum samples for detection of BCHE in Human plasma ? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have low serum quantitys so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2018-04-05
Answer
A: unable to know the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the BCHE ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2018-04-05
