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Product Info Summary
| SKU: | M00261 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-NAK/TBK1 (N-term) Rabbit Monoclonal Antibody
SKU/Catalog Number
M00261
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-NAK/TBK1 (N-term) Rabbit Monoclonal Antibody catalog # M00261. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NAK/TBK1 (N-term) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00261)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AHE-20
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human NAK/TBK1 (N-term)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00261 is reactive to TBK1 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
84 kDa
Calculated molecular weight
83642 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00261 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Positive Control
WB: human Hela whole cell, human 293T whole cell, human MCF-7 whole cell, human K562 whole cell, rat liver tissue, rat lung tissue, mouse liver tissue, mouse lung tissue
IHC: Rat liver tissue, Rat stomach tissue, Human lung tissue, Human colon cancer tissue, Mouse skin tissue, human testis tissue
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded Rat liver, using the Antibody at 1:50 dilution.
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Figure 1. Western blot analysis of NAK/TBK1 using anti-NAK/TBK1 antibody (M00261).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human K562 whole cell lysates,
Lane 5: rat liver tissue lysates,
Lane 6: rat lung tissue lysates,
Lane 7: mouse liver tissue lysates,
Lane 8: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NAK/TBK1 antigen affinity purified monoclonal antibody (Catalog # M00261) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NAK/TBK1 at approximately 84 kDa. The expected band size for NAK/TBK1 is at 84 kDa.
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Immunohistochemical analysis of paraffin-embedded Rat stomach, using the Antibody at 1:50 dilution.
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Immunohistochemical analysis of paraffin-embedded Human lung, using the Antibody at 1:50 dilution.
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Immunohistochemical analysis of paraffin-embedded Human colon cancer, using the Antibody at 1:100 dilution.
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Immunohistochemical analysis of paraffin-embedded Mouse skin, using the Antibody at 1:50 dilution.
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Immunohistochemical analysis of paraffin-embedded human testis, using NAK/TBK1 (N-term) Antibody.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Immunofluorescent analysis of MCF7 cells, using NAK/TBK1 (N-term) Antibody.
Protein Target Info & Infographic
Gene/Protein Information For TBK1 (Source: Uniprot.org, NCBI)
Gene Name
TBK1
Full Name
Serine/threonine-protein kinase TBK1
Weight
83642 MW
Superfamily
protein kinase superfamily
Alternative Names
Serine/threonine-protein kinase TBK1;2.7.11.1;NF-kappa-B-activating kinase;T2K;TANK-binding kinase 1;TBK1;NAK; TBK1 FTDALS4, IIAE8, NAK, T2K TANK binding kinase 1 serine/threonine-protein kinase TBK1|NF-kB-activating kinase|NF-kappa-B-activating kinase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TBK1, check out the TBK1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TBK1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NAK/TBK1 (N-term) Rabbit Monoclonal Antibody (M00261)
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-NAK/TBK1 (N-term) Rabbit Monoclonal Antibody
Question
I was wanting to use your anti-NAK/TBK1 (N-term) Rabbit Monoclonal antibody for IHC for mouse erythroleukemia on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse erythroleukemia identification?
Verified Customer
Verified customer
Asked: 2020-04-23
Answer
You can see on the product datasheet, M00261 anti-NAK/TBK1 (N-term) Rabbit Monoclonal antibody has been tested for IF, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse erythroleukemia in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-23
Question
Will M00261 anti-NAK/TBK1 (N-term) Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-03-30
Answer
You can see on the product datasheet, M00261 anti-NAK/TBK1 (N-term) Rabbit Monoclonal antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-03-30
Question
We want to test anti-NAK/TBK1 (N-term) Rabbit Monoclonal antibody M00261 on mouse erythroleukemia for research purposes, then I may be interested in using anti-NAK/TBK1 (N-term) Rabbit Monoclonal antibody M00261 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-01-17
Answer
The products we sell, including anti-NAK/TBK1 (N-term) Rabbit Monoclonal antibody M00261, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-01-17
Question
We are currently using anti-NAK/TBK1 (N-term) Rabbit Monoclonal antibody M00261 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-21
Answer
The anti-NAK/TBK1 (N-term) Rabbit Monoclonal antibody (M00261) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-21
Question
I have a question about product M00261, anti-NAK/TBK1 (N-term) Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-10-11
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00261 anti-NAK/TBK1 (N-term) Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-11
Question
Would anti-NAK/TBK1 (N-term) Rabbit Monoclonal antibody M00261 work for IHC with erythroleukemia?
Verified Customer
Verified customer
Asked: 2019-07-29
Answer
According to the expression profile of erythroleukemia, TBK1 is highly expressed in erythroleukemia. So, it is likely that anti-NAK/TBK1 (N-term) Rabbit Monoclonal antibody M00261 will work for IHC with erythroleukemia.
Boster Scientific Support
Answered: 2019-07-29
Question
We ordered your anti-NAK/TBK1 (N-term) Rabbit Monoclonal antibody for IHC on erythroleukemia in a previous project. I am using rat, and We are going to use the antibody for IF next. My question regards examining erythroleukemia as well as spleen in our next experiment. Could give a recommendation on which antibody would work the best for IF?
Verified Customer
Verified customer
Asked: 2019-06-12
Answer
I have checked the website and datasheets of our anti-NAK/TBK1 (N-term) Rabbit Monoclonal antibody and it seems that M00261 has been tested on rat in both IHC and IF. Thus M00261 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-06-12
Question
We have observed staining in human erythroleukemia. Any tips? Is anti-NAK/TBK1 (N-term) Rabbit Monoclonal antibody supposed to stain erythroleukemia positively?
Verified Customer
Verified customer
Asked: 2019-05-22
Answer
According to literature erythroleukemia does express TBK1. According to Uniprot.org, TBK1 is expressed in lateral nuclear group of thalamus, spleen, placenta, testis, cervix carcinoma, erythroleukemia, among other tissues. Regarding which tissues have TBK1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18691976
Erythroleukemia, Pubmed ID: 23186163
Placenta, Pubmed ID: 14702039
Spleen, Pubmed ID: 10581243
Testis, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-05-22
Question
Our team were satisfied with the WB result of your anti-NAK/TBK1 (N-term) Rabbit Monoclonal antibody. However we have seen positive staining in spleen cytoplasm using this antibody. Is that expected? Could you tell me where is TBK1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-04-20
Answer
According to literature, spleen does express TBK1. Generally TBK1 expresses in cytoplasm. Regarding which tissues have TBK1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18691976
Erythroleukemia, Pubmed ID: 23186163
Placenta, Pubmed ID: 14702039
Spleen, Pubmed ID: 10581243
Testis, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2018-04-20
Question
See below the WB image, lot number and protocol we used for erythroleukemia using anti-NAK/TBK1 (N-term) Rabbit Monoclonal antibody M00261. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-06-02
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-06-02
Question
Is this M00261 anti-NAK/TBK1 (N-term) Rabbit Monoclonal antibody reactive to the isotypes of TBK1?
Verified Customer
Verified customer
Asked: 2017-05-25
Answer
The immunogen of M00261 anti-NAK/TBK1 (N-term) Rabbit Monoclonal antibody is A synthesized peptide derived from human NAK/TBK1 (N-term). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-05-25
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for erythroleukemia using anti-NAK/TBK1 (N-term) Rabbit Monoclonal antibody M00261. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-05-22
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-05-22
Question
Is a blocking peptide available for product anti-NAK/TBK1 (N-term) Rabbit Monoclonal antibody (M00261)?
M. Rodriguez
Verified customer
Asked: 2017-02-20
Answer
We do provide the blocking peptide for product anti-NAK/TBK1 (N-term) Rabbit Monoclonal antibody (M00261). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-02-20
Question
Is there a BSA free version of anti-NAK/TBK1 (N-term) Rabbit Monoclonal antibody M00261 available?
M. Edwards
Verified customer
Asked: 2016-05-13
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-NAK/TBK1 (N-term) Rabbit Monoclonal antibody M00261 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2016-05-13
Question
I see that the anti-NAK/TBK1 (N-term) Rabbit Monoclonal antibody M00261 works with IHC, what is the protocol used to produce the result images on the product page?
O. Banerjee
Verified customer
Asked: 2015-09-02
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2015-09-02
Question
I am interested in using your anti-NAK/TBK1 (N-term) Rabbit Monoclonal antibody for i-kappab kinase/nf-kappab signaling studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.
B. Zhang
Verified customer
Asked: 2014-04-09
Answer
We appreciate your inquiry. This M00261 anti-NAK/TBK1 (N-term) Rabbit Monoclonal antibody is validated on hela cell lysate. It is guaranteed to work for IF, IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2014-04-09
