Anti-NAK TBK1 Antibody

Product Name

Anti-NAK TBK1 Antibody

View all TBK1 Antibodies

SKU/Catalog Number

A00261

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-NAK TBK1 Antibody (Catalog # A00261). Tested in ELISA, WB, ICC applications. This antibody reacts with Human, Mouse.

Storage & Handling

NAK antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-NAK TBK1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00261)

Host

Rabbit

Contents

NAK Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

NAK antibody was raised against a synthetic peptide corresponding to 17 amino acids form near the carboxy terminus of human NAK/TBK1. The immunogen is located within the last 50 amino acids of NAK.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross response to IKKa, IKKb, IKKy, or IKKe.

Reactive Species

A00261 is reactive to TBK1 in Human, Mouse

Reconstitution

Observed Molecular Weight

68 kDa

Calculated molecular weight

83642 MW

Background of TBK1

Nuclear factor kappa B (NF-κB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-κB is associated with IκB proteins in the cell cytoplasm, which inhibit NF-κB activity. Phosphorylation of I-kappaB by IκB kinase (IKK) complex leads to degradation of I-kappaB and activation of NF-κB. The IKK complex contains IKKα, IKKβ, and IKKγ. A novel IKK related kinase was recently identified and designated TBK1 (TANK-binding kinase 1), NAK (NF-κB-activating kinase), and T2K. NAK/TBK1 activates IKKβ through direct phosphorylation. NAK/TBK1 is activated by growth factors and PMA and mediates IKK and NF-κB activation in response to growth factors. NAK/TBK1 functions upstream of NIK and the IKK complex. NAK/TBK1 is also critical in protecting embryonic liver from apoptosis.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A00261 is guaranteed for ELISA, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

NAK antibody can be used for detection of NAK/TBK1 by Western blot. An 84 kDa band should be detected. Antibody can also be used for immunocytochemistry starting at 10 μg/mL.
Antibody validated: Immunocytochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For TBK1 (Source: Uniprot.org, NCBI)

Alternative Names

NAK, T2K, NAK, NF-kappa-B-activating kinase TBK1 FTDALS4, IIAE8, NAK, T2K TANK binding kinase 1 serine/threonine-protein kinase TBK1|NF-kB-activating kinase|NF-kappa-B-activating kinase

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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