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Product Info Summary
| SKU: | M01577 |
|---|---|
| Size: | 100ug/vial |
| Reactive Species: | Human, Mouse |
| Host: | Mouse |
| Application: | IHC |
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Product info
Product Name
Anti-N-Cadherin-2 CDH2 CD325-Monoclonal Antibody
View all N-Cadherin Antibodies
SKU/Catalog Number
M01577
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-N-Cadherin-2 CDH2 CD325-Monoclonal Antibody (Catalog # M01577). Tested in IHC applications. This antibody reacts with Human, Mouse.
Storage & Handling
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-N-Cadherin-2 CDH2 CD325-Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01577)
Host
Mouse
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Clonality
Monoclonal
Clone Number
Clone: CDH2/1573
Isotype
IgG1, kappa
Immunogen
Recombinant human CDH2 intracellular domain
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Does not cross-react with primate, avian or amphibian GR.
Reactive Species
M01577 is reactive to CDH2 in Human, Mouse
Reconstitution
Reconstitute with distilled water.
Observed Molecular Weight
68 kDa
Calculated molecular weight
99809 MW
Background of N-Cadherin
Recognizes a protein of ~140kDa, identified as N-Cadherin (NCAD), also known as CD325. N-cadherin is a 140 kDa protein belonging to a family of transmembrane molecules that mediate calcium-dependent intercellular adhesion. Cadherins are involved in controlling morphogenetic movements during development and regulate cell surface adhesion through homotypic adhesion with the same cadherin species. Expression of N-cadherin has been reported on a variety of normal tissues including neuronal, endothelial and muscle cells, and a subpopulation of early hematopoietic progenitor cells. Results aid in the classification of malignant non-carcinomatous neoplasms including mesotheliomas, chordomas, synovial sarcomas, malignant melanomas, epithelioid sarcomas, epithelioid angiosarcomas, clear cell sarcomas as well as serous and endometrioid tumors of the ovary have been demonstrated to be N-cadherin positive, whereas mucinous tumors are negative. Other N-cadherin-positive neoplasms include renal cell carcinomas and some variant breast tumors, including medullary breast carcinomas and sarcomatoid metaplastic breast carcinomas.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01577 is guaranteed for IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
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Formalin-fixed, paraffin-embedded Mouse Heart stained with Anti-N-Cadherin Mouse Monoclonal Antibody (CDH2/1573).
Protein Target Info & Infographic
Gene/Protein Information For CDH2 (Source: Uniprot.org, NCBI)
Gene Name
CDH2
Full Name
Cadherin-2
Weight
99809 MW
Alternative Names
Cadherin-2;CDw325;Neural cadherin;N-cadherin;CD325;CDH2;CDHN, NCAD; CDH2 ACOGS, ARVD14, CD325, CDHN, CDw325, NCAD cadherin 2 cadherin-2|N-cadherin 1|cadherin 2, type 1, N-cadherin (neuronal)|calcium-dependent adhesion protein, neuronal|neural cadherin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CDH2, check out the CDH2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CDH2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-N-Cadherin-2 CDH2 CD325-Monoclonal Antibody (M01577)
Hello CJ!
M01577 has been cited in 8 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Shi R,Liu L,Wang F,He Y,Niu Y,Wang C,Zhang X,Zhang X,Zhang H,Chen M,Wang Y.Downregulation of cytokeratin 18 induces cellular partial EMT and stemness through increasing EpCAM expression in breast cancer.Cell Signal.2020 Dec;76:109810.doi:10.1016/j.cellsig
Species: Human
M01577 usage in article: APP:IF, SAMPLE:BREAST CANCER CELLS AND BREAST TISSUE, DILUTION:1:100
Expression of N-cadherin in myocardial tissues during the development of a rat heart
Ionizing radiation promotes migration and invasion of cancer cells through transforming growth factor-beta%u2013mediated epithelial%u2013mesenchymal transition
Wnt/?-catenin pathway is required for epithelial to mesenchymal transition in CXCL12 over expressed breast cancer cells
RAC1 overexpression promotes the proliferation, migration and epithelial-mesenchymal transition of lens epithelial cells
MiR-506 inhibits multiple targets in the epithelial-to-mesenchymal transition network and is associated with good prognosis in epithelial ovarian cancer
Celastrol Ameliorates Ulcerative Colitis-Related Colorectal Cancer in Mice via Suppressing Inflammatory Responses and Epithelial-Mesenchymal Transition
Qiao B, Johnson Nw, Gao J. Int J Oncol. 2010 Sep;37(3):663-8. Epithelial-Mesenchymal Transition In Oral Squamous Cell Carcinoma Triggered By Transforming Growth Factor-Beta1 Is Snail Family-Dependent And Correlates With Matrix Metalloproteinase-2 ...
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