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Product Info Summary
| SKU: | M00163-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-SLC2A1 Antibody Picoband® (monoclonal, 10C10)
SKU/Catalog Number
M00163-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SLC2A1 Antibody Picoband® (monoclonal, 10C10) catalog # M00163-1. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SLC2A1 Antibody Picoband® (monoclonal, 10C10) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00163-1)
Host
Mouse
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Monoclonal
Clone Number
10C10
Isotype
Mouse IgG1
Immunogen
E.coli-derived human SLC2A1 recombinant protein (Position: R92-V492). Human SLC2A1 shares 98% and 98.3% amino acid (aa) sequence identity with mouse and rat SLC2A1, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
M00163-1 is reactive to SLC2A1 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
Observed Molecular Weight
55 kDa
Calculated molecular weight
46659 MW
Background of Glut1
GLUT1, also known as SLC2A1, is a major glucose transporter in the mammalian blood-brain barrier whose gene is mapped to 1p35-p31.3 and contains 10 exons. It is present at high levels in primate erythrocytes and brain endothelial cells. Not only can transport dehydroascorbic acid (the oxidized form of vitamin C) into the brain, GLUT1 is also likely to contribute to HTLV-associated disorders through interacting with HTLV envelope glycoproteins. Functionally, GLUT1 deficiency causes a decrease in embryonic glucose uptake and apoptosis, which may be involved in diabetic embryopathy, by contrast, an increased expression of GLUT1 in some malignant tumors may suggest a role for glucose-derivative tracers to detect in vivo thyroid cancer metastases by positron-emission tomography scanning.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00163-1 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Immunocytochemistry/Immunofluorescence, 5μg/ml
Flow Cytometry (Fixed), 1-3μg/1x106 cells
Positive Control
WB: human placenta tissue, human placenta tissue
IHC: human placenta tissue, human renal cancer tissue
ICC/IF: SiHa cell
FCM: U20S cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of SLC2A1 using anti-SLC2A1 antibody (M00163-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human placenta tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-SLC2A1 antigen affinity purified monoclonal antibody (Catalog # M00163-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for SLC2A1 at approximately 55KD. The expected band size for SLC2A1 is at 55KD.
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Figure 2. IHC analysis of SLC2A1 using anti-SLC2A1 antibody (M00163-1).
SLC2A1 was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-SLC2A1 Antibody (M00163-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of SLC2A1 using anti-SLC2A1 antibody (M00163-1).
SLC2A1 was detected in paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-SLC2A1 Antibody (M00163-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Click image to see more details
Figure 4. IF analysis of SLC2A1 using anti-SLC2A1 antibody (M00163-1).
SLC2A1 was detected in immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL mouse anti-SLC2A1 Antibody (M00163-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Figure 5. Flow Cytometry analysis of U20S cells using anti- SLC2A1 antibody (M00163-1).
Overlay histogram showing U20S cells stained with M00163-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-SLC2A1 Antibody (M00163-1, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For SLC2A1 (Source: Uniprot.org, NCBI)
Gene Name
SLC2A1
Full Name
Solute carrier family 2, facilitated glucose transporter member 1
Weight
46659 MW
Superfamily
major facilitator superfamily
Alternative Names
Solute carrier family 2, facilitated glucose transporter member 1; Glucose transporter type 1, erythrocyte/brain; GLUT-1; HepG2 glucose transporter; SLC2A1; GLUT1 SLC2A1 CSE, DYT17, DYT18, DYT9, EIG12, GLUT, GLUT-1, GLUT1, GLUT1DS, HTLVR, PED, SDCHCN solute carrier family 2 member 1 solute carrier family 2, facilitated glucose transporter member 1|choreoathetosis/spasticity, episodic (paroxysmal choreoathetosis/spasticity)|glucose transporter type 1, erythrocyte/brain|hepG2 glucose transporter|human T-cell leukemia virus (I and II) receptor|receptor for HTLV-1 and HTLV-2|solute carrier family 2 (facilitated glucose transporter), member 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SLC2A1, check out the SLC2A1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SLC2A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SLC2A1 Antibody Picoband® (monoclonal, 10C10) (M00163-1)
Hello CJ!
M00163-1 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Co-Inhibition of GLUT-1 Expression and the PI3K/Akt Signaling Pathway to Enhance the Radiosensitivity of Laryngeal Carcinoma Xenografts?In Vivo
Apigenin inhibits the proliferation of adenoid cystic carcinoma via suppression of glucose transporter-1
Xu Yy, Wu Tt, Zhou Sh, Bao Yy, Wang Qy, Fan J, Huang Yp. Int J Clin Exp Pathol. 2014 Jun 15;7(7):3938-47. Ecollection 2014. Apigenin Suppresses Glut-1 And P-Akt Expression To Enhance The Chemosensitivity To Cisplatin Of Laryngeal Carcinoma Hep-2 C...
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-SLC2A1 Antibody Picoband® (monoclonal, 10C10)
Question
I am interested in to test anti-SLC2A1 antibody (monoclonal, 10C10) M00163-1 on human cervix carcinoma for research purposes, then I may be interested in using anti-SLC2A1 antibody (monoclonal, 10C10) M00163-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-04-20
Answer
The products we sell, including anti-SLC2A1 antibody (monoclonal, 10C10) M00163-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-04-20
Question
My team were content with the WB result of your anti-SLC2A1 antibody (monoclonal, 10C10). However we have seen positive staining in articular cartilage cell membrane using this antibody. Is that expected? Could you tell me where is SLC2A1 supposed to be expressed?
G. Anderson
Verified customer
Asked: 2020-02-21
Answer
From literature, articular cartilage does express SLC2A1. Generally SLC2A1 expresses in cell membrane. Regarding which tissues have SLC2A1 expression, here are a few articles citing expression in various tissues:
Brain, and Trachea, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19349973
Liver, Pubmed ID: 24275569
Melanoma, Pubmed ID: 17081065
Boster Scientific Support
Answered: 2020-02-21
Question
We have observed staining in human melanoma. Do you have any suggestions? Is anti-SLC2A1 antibody (monoclonal, 10C10) supposed to stain melanoma positively?
Verified Customer
Verified customer
Asked: 2020-02-07
Answer
According to literature melanoma does express SLC2A1. According to Uniprot.org, SLC2A1 is expressed in pigmented layer of retina, brain trachea, brain, articular cartilage, melanoma, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have SLC2A1 expression, here are a few articles citing expression in various tissues:
Brain, and Trachea, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19349973
Liver, Pubmed ID: 24275569
Melanoma, Pubmed ID: 17081065
Boster Scientific Support
Answered: 2020-02-07
Question
Do you have a BSA free version of anti-SLC2A1 antibody (monoclonal, 10C10) M00163-1 available?
Verified Customer
Verified customer
Asked: 2020-01-24
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-SLC2A1 antibody (monoclonal, 10C10) M00163-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-01-24
Question
Is a blocking peptide available for product anti-SLC2A1 antibody (monoclonal, 10C10) (M00163-1)?
Verified Customer
Verified customer
Asked: 2019-12-02
Answer
We do provide the blocking peptide for product anti-SLC2A1 antibody (monoclonal, 10C10) (M00163-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-12-02
Question
We bought anti-SLC2A1 antibody (monoclonal, 10C10) for IHC-P on brain trachea in the past. I am using human, and We want to use the antibody for WB next. I was wanting to use examining brain trachea as well as pigmented layer of retina in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2019-11-26
Answer
I looked at the website and datasheets of our anti-SLC2A1 antibody (monoclonal, 10C10) and I see that M00163-1 has been tested on human in both IHC-P and WB. Thus M00163-1 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-11-26
Question
Is this M00163-1 anti-SLC2A1 antibody (monoclonal, 10C10) reactive to the isotypes of SLC2A1?
Verified Customer
Verified customer
Asked: 2019-11-14
Answer
The immunogen of M00163-1 anti-SLC2A1 antibody (monoclonal, 10C10) is E.coli-derived human SLC2A1 recombinant protein (Position: R92-V492). Human SLC2A1 shares 98% and 98.3% amino acid (aa) sequence identity with mouse and rat SLC2A1, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-11-14
Question
I was wanting to use your anti-SLC2A1 antibody (monoclonal, 10C10) for IHC-P for human cervix carcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human cervix carcinoma identification?
Verified Customer
Verified customer
Asked: 2019-06-03
Answer
As indicated on the product datasheet, M00163-1 anti-SLC2A1 antibody (monoclonal, 10C10) has been tested for IHC-P, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human cervix carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-03
Question
Please see the WB image, lot number and protocol we used for cervix carcinoma using anti-SLC2A1 antibody (monoclonal, 10C10) M00163-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-05-06
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-06
Question
We are currently using anti-SLC2A1 antibody (monoclonal, 10C10) M00163-1 for human tissue, and we are well pleased with the IHC-P results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on monkey tissues as well?
M. Parker
Verified customer
Asked: 2018-08-24
Answer
The anti-SLC2A1 antibody (monoclonal, 10C10) (M00163-1) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-08-24
Question
Can you help my question with product M00163-1, anti-SLC2A1 antibody (monoclonal, 10C10). I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
C. Wu
Verified customer
Asked: 2018-01-31
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00163-1 anti-SLC2A1 antibody (monoclonal, 10C10), we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-01-31
Question
Would anti-SLC2A1 antibody (monoclonal, 10C10) M00163-1 work for IHC-P with cervix carcinoma?
Verified Customer
Verified customer
Asked: 2017-12-07
Answer
According to the expression profile of cervix carcinoma, SLC2A1 is highly expressed in cervix carcinoma. So, it is likely that anti-SLC2A1 antibody (monoclonal, 10C10) M00163-1 will work for IHC-P with cervix carcinoma.
Boster Scientific Support
Answered: 2017-12-07
Question
Will M00163-1 anti-SLC2A1 antibody (monoclonal, 10C10) work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-07-05
Answer
It shows on the product datasheet, M00163-1 anti-SLC2A1 antibody (monoclonal, 10C10) as been tested on IHC-P. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-07-05
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-SLC2A1 antibody (monoclonal, 10C10) M00163-1. Let me know if you need anything else.
N. Krishna
Verified customer
Asked: 2016-10-25
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-10-25
Question
I see that the anti-SLC2A1 antibody (monoclonal, 10C10) M00163-1 works with IHC-P, what is the protocol used to produce the result images on the product page?
A. Anderson
Verified customer
Asked: 2014-01-14
Answer
You can find protocols for IHC-P on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2014-01-14
