Anti-Interleukin-3 IL3 Antibody Picoband®

IL-3 antibody

Boster Bio Anti-Interleukin-3 IL3 Antibody catalog # PA1728. Tested in WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Info Summary

SKU: PA1728
Size: 100 μg/vial
Reactive Species: Mouse
Host: Rabbit
Application: WB

Product Name

Anti-Interleukin-3 IL3 Antibody Picoband®

View all IL-3 Antibodies

SKU/Catalog Number

PA1728

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Interleukin-3 IL3 Antibody catalog # PA1728. Tested in WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Interleukin-3 IL3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1728)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of mouse IL-3.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1728 is reactive to Il3 in Mouse

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

14 kDa

Calculated molecular weight

18540 MW

Background of IL-3

Interleukin 3, also known as IL-3, is a protein that in humans is encoded by the IL3 gene. Ot is mapped to 5q31.1. IL-3 is an interleukin, a type of biological signal (cytokine) that can improve the body's natural response to disease as part of the immune system. It acts by binding to the interleukin-3 receptor. IL-3 stimulates the differentiation of multipotent hematopoietic stem cells into myeloid progenitor cells or, with the addition of IL-7, into lymphoid progenitor cells. In addition, IL-3 stimulates proliferation of all cells in the myeloid lineage (granulocytes, monocytes, and dendritic cells), in conjunction with other cytokines, e.g., Erythropoietin (EPO), Granulocyte macrophage colony-stimulating factor (GM-CSF), and IL-6. IL-3 is secreted by basophils and activated T cells to support growth and differentiation of T cells from the bone marrow in an immune response.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

PA1728 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Mouse

Validation Images & Assay Conditions

Gene/Protein Information For Il3 (Source: Uniprot.org, NCBI)

Gene Name

Il3

Full Name

Interleukin-3

Weight

18540 MW

Superfamily

IL-3 family

Alternative Names

Interleukin-3;IL-3;Hematopoietic growth factor;Mast cell growth factor;MCGF;Multipotential colony-stimulating factor;P-cell-stimulating factor;Il3;Csfmu, Il-3; IL3 IL-3, MCGF, MULTI-CSF interleukin 3 interleukin-3|P-cell stimulating factor|colony-stimulating factor, multiple|hematopoietic growth factor|mast-cell growth factor|multilineage-colony-stimulating factor|multipotential colony-stimulating factor

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Il3, check out the Il3 Infographic

Il3 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Il3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

No publications found for PA1728

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used Anti-Interleukin-3 IL3 Antibody Picoband®?

Submit a review and receive an Amazon gift card.

  • $30 for a review with an image

0 Reviews For Anti-Interleukin-3 IL3 Antibody Picoband®

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

6 Customer Q&As for Anti-Interleukin-3 IL3 Antibody Picoband®

Question

I was wanting to use your anti-IL3 antibody for WB for mouse right lobe of liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse right lobe of liver identification?

S. Edwards

Verified customer

Asked: 2020-03-10

Answer

It shows on the product datasheet, PA1728 anti-IL3 antibody has been validated for WB on mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse right lobe of liver in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-03-10

Question

Does anti-IL3 antibody PA1728 work for WB with right lobe of liver?

Verified Customer

Verified customer

Asked: 2019-12-11

Answer

According to the expression profile of right lobe of liver, IL3 is highly expressed in right lobe of liver. So, it is likely that anti-IL3 antibody PA1728 will work for WB with right lobe of liver.

Boster Scientific Support

Answered: 2019-12-11

Question

See attached the WB image, lot number and protocol we used for right lobe of liver using anti-IL3 antibody PA1728. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2018-02-06

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-02-06

Question

Is this PA1728 anti-IL3 antibody reactive to the isotypes of IL3?

R. Bhatt

Verified customer

Asked: 2016-12-28

Answer

The immunogen of PA1728 anti-IL3 antibody is A synthetic peptide corresponding to a sequence in the middle region of mouse IL-3(122-140aa, RDLDDFRKKLRFYMVHLND). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2016-12-28

Question

Can you help my question with product PA1728, anti-IL3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

P. Brown

Verified customer

Asked: 2014-03-07

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1728 anti-IL3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2014-03-07

Question

Do you have a BSA free version of anti-IL3 antibody PA1728 available?

J. Wu

Verified customer

Asked: 2013-12-10

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-IL3 antibody PA1728 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2013-12-10

Order DetailsPrice
PA1728

100μg

$370
PA1728-10ug

10μg sample (liquid)

$99
PA1728-Biotin

100 μg Biotin conjugated

$570
PA1728-Cy3

100 μg Cy3 conjugated

$570
PA1728-Dylight488

100 μg Dylight488 conjugated

$570
PA1728-Dylight550

100 μg Dylight550 conjugated

$570
PA1728-Dylight594

100 μg Dylight594 conjugated

$570
PA1728-FITC

100 μg FITC conjugated

$570
PA1728-HRP

100 μg HRP conjugated

$570
PA1728-APC

100 μg APC conjugated

$670
PA1728-PE

100 μg PE conjugated

$670
PA1728-iFluor647

100 μg iFluor647 conjugated

$670
PA1728-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

Choose an option to show lead time.
Get A Quote
In stock
Order Product
PA1728
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.