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Product Info Summary
| SKU: | MA1064 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Monkey, Mouse, Rat |
| Host: | Mouse |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Myosin(Skeletal, Slow) Myh7 Antibody (Monoclonal, NOQ7.5.4D)
SKU/Catalog Number
MA1064
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Myosin (Skeletal, Slow) Myh7 Antibody (Monoclonal, NOQ7.5.4D) catalog # MA1064. Tested in IHC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Myosin(Skeletal, Slow) Myh7 Antibody (Monoclonal, NOQ7.5.4D) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1064)
Host
Mouse
Contents
Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Clonality
Monoclonal
Clone Number
NOQ7.5.4D
Isotype
Mouse IgG1
Immunogen
Human skeletal muscle myosin purified from myofibrils.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
MA1064 is reactive to Myh7 in Human, Monkey, Mouse, Rat
Reconstitution
Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Observed Molecular Weight
230 kDa
Calculated molecular weight
223097 MW
Background of MYH7
Myosin is composed of 2 heavy chains of about 200,000 daltons each and 4 light chains of about 20,000 daltons each. Skeletal Myosin (slow),, also known as light chain 3 (MYL3), mapped to 3p. Fodor et al. (1989) found that the MYL3 gene has 7 exons, the last of which is completely untranslated 3-prime sequence.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
MA1064 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.5-2μg/ml, Human, monkey, mouse, rat
Immunohistochemistry (Paraffin-embedded Section), 1-2μg/ml, Human, monkey, mouse, rat, By Heat
Positive Control
WB: monkey heart tissue, monkey skeletal muscle tissue, rat heart tissue, rat skeletal muscle tissue, mouse heart tissue, mouse skeletal muscle tissue
IHC: rat skeletal muscle tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Myosin using anti-Myosin antibody (MA1064).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: monkey heart tissue lysates,
Lane 2: monkey skeletal muscle tissue lysates,
Lane 3: rat heart tissue lysates,
Lane 4: rat skeletal muscle tissue lysates,
Lane 5: mouse heart tissue lysates,
Lane 6: mouse skeletal muscle tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Myosin antigen affinity purified monoclonal antibody (Catalog # MA1064) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Myosin at approximately 230 kDa. The expected band size for Myosin is at 223 kDa.
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Figure 2. IHC analysis of Myosin using anti-Myosin antibody (MA1064).
Myosin was detected in a paraffin-embedded section of rat skeletal muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Myosin Antibody (MA1064) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For Myh7 (Source: Uniprot.org, NCBI)
Gene Name
Myh7
Full Name
Myosin-7
Weight
223097 MW
Superfamily
TRAFAC class myosin-kinesin ATPase superfamily
Alternative Names
Myosin-7;Myosin heavy chain 7;Myosin heavy chain slow isoform;MyHC-slow;Myosin heavy chain, cardiac muscle beta isoform;MyHC-beta;Myh7; MYH7 CMD1S, CMH1, MPD1, MYHCB, SPMD, SPMM myosin heavy chain 7 myosin-7|cardiac muscle myosin heavy chain 7 beta|myHC-beta|myhc-slow|myopathy, distal 1|myosin 7|myosin heavy chain beta-subunit|myosin, heavy chain 7, cardiac muscle, beta|myosin, heavy polypeptide 7, cardiac muscle, beta|rhabdomyosarcoma MU-RMS-40.7A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Myh7, check out the Myh7 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Myh7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Myosin(Skeletal, Slow) Myh7 Antibody (Monoclonal, NOQ7.5.4D) (MA1064)
Hello CJ!
MA1064 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Yan X,Niu Q,Gao X,Shen S,He N,Wang H,Fang R,Gao Y,Chang H.2021.Differential protein metabolism and regeneration in gastrocnemius muscles in high-fat diet fed mice and pre-hibernation Daurian ground squirrels: a comparison between pathological and healthy
Species: Mouse
Nie Yw, Ding Xb, Ge Xg, Fan Hl, Liu Zw, Guo H. Cell Biol Int. 2013 Sep;37(9):972-6. Doi: 10.1002/Cbin.10124. Epub 2013 May 23. Enhanced Expression Of Myf5 And Myod1 In Fibroblast Cells Via The Forced Expression Of Bos Taurus Myf5.
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Customer Q&As
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7 Customer Q&As for Anti-Myosin(Skeletal, Slow) Myh7 Antibody (Monoclonal, NOQ7.5.4D)
Question
Is a blocking peptide available for product anti-Myosin(Skeletal, Slow) antibody (Monoclonal, NOQ7.5.4D) (MA1064)?
Verified Customer
Verified customer
Asked: 2020-02-19
Answer
We do provide the blocking peptide for product anti-Myosin(Skeletal, Slow) antibody (Monoclonal, NOQ7.5.4D) (MA1064). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-02-19
Question
We are currently using anti-Myosin(Skeletal, Slow) antibody (Monoclonal, NOQ7.5.4D) MA1064 for mouse tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse, rabbit, rat. Is it likely that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-04
Answer
The anti-Myosin(Skeletal, Slow) antibody (Monoclonal, NOQ7.5.4D) (MA1064) has not been tested for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-04
Question
Could you please clarify the MA1064 target? The antibody is titled 'Anti-Myosin(Skeletal, Slow) Antibody (Monoclonal, NOQ7.5.4D)', but the accession number given on the datasheet is https://www.uniprot.org/uniprot/P12883 MYH7 alternated names Myosin-7;Myosin heavy chain 7;Myosin heavy chain slow isoform;MyHC-slow;Myosin heavy chain, cardiac muscle beta isoform;MyHC-beta;Myh7; There is no mention of skeletal muscle on the Uniprot page.
Verified customer
Asked: 2019-07-11
Answer
The target for the Anti-Myosin(Skeletal, Slow) Myh7 Antibody (Monoclonal, NOQ7.5.4D) (MA1064) is correct (Uniprot : P12883). Slow myosin is common to skeletal as well as others such as cardiac muscles. This is probably why there is no mention of skeletal muscle on the Uniprot page. This clone recognizes an epitope located on the heavy meromyosin portion of human adult skeletal muscle slow myosin.
Boster Scientific Support
Answered: 2019-07-15
Question
I was wanting to use your anti-Myosin(Skeletal, Slow) antibody (Monoclonal, NOQ7.5.4D) for IHC for mouse skeletal muscle on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse skeletal muscle identification?
R. Rodriguez
Verified customer
Asked: 2018-10-02
Answer
You can see on the product datasheet, MA1064 anti-Myosin(Skeletal, Slow) antibody (Monoclonal, NOQ7.5.4D) has been tested for IHC, WB on human, mouse, rabbit, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse skeletal muscle in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-10-02
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for skeletal muscle using anti-Myosin(Skeletal, Slow) antibody (Monoclonal, NOQ7.5.4D) MA1064. Let me know if you need anything else.
K. Dhar
Verified customer
Asked: 2017-07-03
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-07-03
Question
Here is the WB image, lot number and protocol we used for skeletal muscle using anti-Myosin(Skeletal, Slow) antibody (Monoclonal, NOQ7.5.4D) MA1064. Please let me know if you require anything else.
E. Zhao
Verified customer
Asked: 2016-03-24
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-03-24
Question
Do you have a BSA free version of anti-Myosin(Skeletal, Slow) antibody (Monoclonal, NOQ7.5.4D) MA1064 available?
J. Wu
Verified customer
Asked: 2013-04-02
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Myosin(Skeletal, Slow) antibody (Monoclonal, NOQ7.5.4D) MA1064 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2013-04-02
