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Product Info Summary
| SKU: | M04058 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Myoglobin MB Rabbit Monoclonal Antibody
SKU/Catalog Number
M04058
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Myoglobin MB Rabbit Monoclonal Antibody catalog # M04058. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Myoglobin MB Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04058)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
FOD-13
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Myoglobin
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M04058 is reactive to MB in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
15 kDa
Calculated molecular weight
17184 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04058 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:30
Positive Control
WB: Human heart muscle, rat heart tissue, rat skeletal muscle tissue, mouse heart tissue, mouse skeletal muscle tissue
IHC: human skeletal muscle tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of MB using anti-MB antibody (M04058).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat heart tissue lysates,
Lane 2: rat skeletal muscle tissue lysates,
Lane 3: mouse heart tissue lysates,
Lane 4: mouse skeletal muscle tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MB antigen affinity purified monoclonal antibody (Catalog # M04058) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MB at approximately 15 kDa. The expected band size for MB is at 17 kDa.
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Western blot analysis of Myoglobin expression in Human heart muscle lysate.
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Immunohistochemical analysis of paraffin-embedded human skeletal muscle, using Myoglobin Antibody.
Protein Target Info & Infographic
Gene/Protein Information For MB (Source: Uniprot.org, NCBI)
Gene Name
MB
Full Name
Myoglobin
Weight
17184 MW
Superfamily
globin family
Alternative Names
Myoglobin;MB; MB PVALB myoglobin myoglobin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MB, check out the MB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Myoglobin MB Rabbit Monoclonal Antibody (M04058)
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No publications found for M04058
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Customer Q&As
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14 Customer Q&As for Anti-Myoglobin MB Rabbit Monoclonal Antibody
Question
I was wanting to use your anti-Myoglobin Rabbit Monoclonal antibody for WB for human skeletal muscle on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human skeletal muscle identification?
K. Krishna
Verified customer
Asked: 2020-02-18
Answer
It shows on the product datasheet, M04058 anti-Myoglobin Rabbit Monoclonal antibody has been validated for IP, IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human skeletal muscle in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-18
Question
I see that the anti-Myoglobin Rabbit Monoclonal antibody M04058 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-02-10
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-02-10
Question
Is a blocking peptide available for product anti-Myoglobin Rabbit Monoclonal antibody (M04058)?
Verified Customer
Verified customer
Asked: 2020-01-02
Answer
We do provide the blocking peptide for product anti-Myoglobin Rabbit Monoclonal antibody (M04058). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-01-02
Question
Is this M04058 anti-Myoglobin Rabbit Monoclonal antibody reactive to the isotypes of MB?
C. Anderson
Verified customer
Asked: 2019-12-11
Answer
The immunogen of M04058 anti-Myoglobin Rabbit Monoclonal antibody is A synthesized peptide derived from human Myoglobin. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-12-11
Question
See below the WB image, lot number and protocol we used for skeletal muscle using anti-Myoglobin Rabbit Monoclonal antibody M04058. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-10-03
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-03
Question
Would M04058 anti-Myoglobin Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-07-16
Answer
You can see on the product datasheet, M04058 anti-Myoglobin Rabbit Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-07-16
Question
We have tried in the past anti-Myoglobin Rabbit Monoclonal antibody for WB on heart in the past. I am using mouse, and We are going to use the antibody for IF next. We are interested in examining heart as well as heart right ventricle in our next experiment. Could you please give me some suggestion on which antibody would work the best for IF?
Verified Customer
Verified customer
Asked: 2019-05-07
Answer
I have checked the website and datasheets of our anti-Myoglobin Rabbit Monoclonal antibody and it appears that M04058 has been validated on mouse in both WB and IF. Thus M04058 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-05-07
Question
My lab would like to test anti-Myoglobin Rabbit Monoclonal antibody M04058 on human skeletal muscle for research purposes, then I may be interested in using anti-Myoglobin Rabbit Monoclonal antibody M04058 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
L. Collins
Verified customer
Asked: 2019-03-04
Answer
The products we sell, including anti-Myoglobin Rabbit Monoclonal antibody M04058, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-03-04
Question
Would anti-Myoglobin Rabbit Monoclonal antibody M04058 work for WB with skeletal muscle?
Verified Customer
Verified customer
Asked: 2017-12-01
Answer
According to the expression profile of skeletal muscle, MB is highly expressed in skeletal muscle. So, it is likely that anti-Myoglobin Rabbit Monoclonal antibody M04058 will work for WB with skeletal muscle.
Boster Scientific Support
Answered: 2017-12-01
Question
We are currently using anti-Myoglobin Rabbit Monoclonal antibody M04058 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2017-05-24
Answer
The anti-Myoglobin Rabbit Monoclonal antibody (M04058) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-05-24
Question
Is there a BSA free version of anti-Myoglobin Rabbit Monoclonal antibody M04058 available?
H. Johnson
Verified customer
Asked: 2016-05-24
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Myoglobin Rabbit Monoclonal antibody M04058 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2016-05-24
Question
Can you help my question with product M04058, anti-Myoglobin Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
G. Parker
Verified customer
Asked: 2016-03-15
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M04058 anti-Myoglobin Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2016-03-15
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for skeletal muscle using anti-Myoglobin Rabbit Monoclonal antibody M04058. Let me know if you need anything else.
E. Dhar
Verified customer
Asked: 2015-11-05
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-11-05
Question
We have been able to see staining in human heart. Are there any suggestions? Is anti-Myoglobin Rabbit Monoclonal antibody supposed to stain heart positively?
A. Jones
Verified customer
Asked: 2015-07-29
Answer
According to literature heart does express MB. According to Uniprot.org, MB is expressed in heart right ventricle, skeletal muscle, heart, among other tissues. Regarding which tissues have MB expression, here are a few articles citing expression in various tissues:
Heart, Pubmed ID: 7895732
Skeletal muscle, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2015-07-29
