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Product Info Summary
| SKU: | A01391-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | ELISA |
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Product info
Product Name
Anti-Tff1 Antibody
SKU/Catalog Number
A01391-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Tff1 Antibody catalog # A01391-1. Tested in ELISA applications. This antibody reacts with Mouse.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Tff1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01391-1)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived mouse Tff1 recombinant protein (Position: Q22-F87). Mouse Tff1 shares 67.2% and 83.6% amino acid (aa) sequence identity with human and rat Tff1, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01391-1 is reactive to Tff1 in Mouse
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
17 kDa
Calculated molecular weight
9670 MW
Background of TFF1/pS2
TFF1 (Trefoil factor 1), also known as pS2, is a protein that in humans is encoded by the TFF1 gene. Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer, and affect healing of the epithelium. It is found that TFF1 in normal human urine inhibited the growth of calcium oxalate crystals. Urinary TFF1 showed an inhibitory potency similar to that of nephrocalcin, and inhibition was dose dependent and inhibited by TFF1 antisera, particularly by antisera directed to the TFF1 C terminus. Concentrations and relative amounts of TFF1 in the urine of patients with idiopathic calcium oxalate kidney stones were significantly less than those found in controls. This gene, which is expressed in the gastric mucosa, has also been studied because of its expression in human tumors. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01391-1 is guaranteed for ELISA Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA Capture (Matched Antibody Pair), 2-8μg/ml
Validation Images & Assay Conditions
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Figure 1. Sandwich ELISA - Recombinant mouse Tff1 protein standard curve.
Use in combination with reagents from Mouse Tff1 ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ1620).
Protein Target Info & Infographic
Gene/Protein Information For Tff1 (Source: Uniprot.org, NCBI)
Gene Name
Tff1
Full Name
Trefoil factor 1
Weight
9670 MW
Alternative Names
Trefoil factor 1;Protein pS2;Tff1;Bcei, Ps2; TFF1 BCEI, D21S21, HP1.A, HPS2, pNR-2, pS2 trefoil factor 1 trefoil factor 1|breast cancer estrogen-inducible protein|breast cancer estrogen-inducible sequence|gastrointestinal trefoil protein pS2|polypeptide P1.A|protein pS2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Tff1, check out the Tff1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Tff1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Tff1 Antibody (A01391-1)
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