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Product Info Summary
| SKU: | PB9145 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC |
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Product info
Product Name
Anti-MUC1 Antibody
SKU/Catalog Number
PB9145
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MUC1 Antibody catalog # PB9145. Tested in IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MUC1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9145)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human MUC1 recombinant protein (Position: R945-G1097). Human MUC1 shares 56% amino acid (aa) sequence identity with mouse MUC1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9145 is reactive to MUC1 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
47 kDa
Calculated molecular weight
122.102kDa
Background of MUC1
Mucin 1, cell surface associated (MUC1) or polymorphic epithelial mucin (PEM) is a mucin encoded by the MUC1 gene in humans. This gene encodes a membrane-bound protein that is a member of the mucin family. Mucins are O-glycosylated proteins that play an essential role in forming protective mucous barriers on epithelial surfaces. It is mapped to 1q22. Mucin 1 is a transmembrane mucin normally expressed on the apical borders of secretory epithelial cells. Overexpression of Mucin 1 is often associated with colon, breast, ovarian, lung and pancreatic cancers. The protein serves a protective function by binding to pathogens and also functions in a cell signaling capacity. Mucin 1 stimulated ESR1-mediated transcription and contributed to estradiol-mediated growth and survival of breast cancer cells. This gene also can suppress pulmonary innate immunity, and its antiinflammatory activity may play an important modulatory role during microbial infection.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9145 is guaranteed for IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Positive Control
IHC: human ovary cancer tissue
Validation Images & Assay Conditions
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Figure 1. IHC analysis of MUC1 using anti-MUC1 antibody (PB9145).
MUC1 was detected in a paraffin-embedded section of human ovary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-MUC1 Antibody (PB9145) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For MUC1 (Source: Uniprot.org, NCBI)
Gene Name
MUC1
Full Name
Mucin-1
Weight
122.102kDa
Alternative Names
ADMCKD antibody;ADMCKD1 antibody;Breast carcinoma associated antigen DF3 antibody;Breast carcinoma-associated antigen DF3 antibody;CA 15-3 antibody;CA15 3 antibody;CA15 3 antigen antibody;CA15.3 antibody;Cancer antigen 15-3 antibody;Carcinoma associated mucin antibody;Carcinoma-associated mucin antibody;CD 227 antibody;CD227 antibody;DF3 antigen antibody;EMA antibody;Episialin antibody;H23 antigen antibody;H23AG antibody;KL 6 antibody;KL-6 antibody;KL6 antibody;Krebs von den Lungen-6 antibody;MAM 6 antibody;MAM6 antibody;MCD antibody;MCKD antibody;MCKD1 antibody;Medullary cystic kidney disease 1 (autosomal dominant) antibody;MUC 1 antibody;MUC-1 antibody;MUC-1/SEC antibody;MUC-1/X antibody;MUC1 antibody;MUC1-alpha antibody;MUC1-beta antibody;MUC1-CT antibody;MUC1-NT antibody;MUC1/ZD antibody;MUC1_HUMAN antibody;Mucin 1 antibody;Mucin 1 transmembrane antibody;Mucin 1, cell surface associated antibody;Mucin-1 subunit beta antibody;Peanut reactive urinary mucin antibody;Peanut-reactive urinary mucin antibody;PEM antibody;PEMT antibody;Polymorphic epithelial mucin antibody;PUM antibody;Tumor associated epithelial membrane antigen antibody;Tumor associated epithelial mucin antibody;Tumor associated mucin antibody;Tumor-associated epithelial membrane antigen antibody;Tumor-associated mucin antibody MUC1 ADMCKD, ADMCKD1, ADTKD2, CA 15-3, CD227, EMA, H23AG, KL-6, MAM6, MCD, MCKD, MCKD1, MUC-1, MUC-1/SEC, MUC-1/X/ZD, PEM, PEMT, PUM, MUC1 mucin 1, cell surface associated mucin-1|H23 |breast carcinoma-associated DF3|cancer 15-3|carcinoma-associated mucin|episialin|krebs von den Lungen-6|mucin 1, transmembrane|peanut-reactive urinary mucin|polymorphic epithelial mucin|tumor associated epithelial mucin|tumor-associated epithelial membrane
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MUC1, check out the MUC1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MUC1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MUC1 Antibody (PB9145)
Hello CJ!
PB9145 has been cited in 18 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Combination of fasudil and celecoxib promotes the recovery of injured spinal cord in rats better than celecoxib or fasudil alone
Puerarin protects brain tissue against cerebral ischemia/reperfusion injury by inhibiting the inflammatory response
Effect of cyclooxygenase-2 inhibition on the development of post-traumatic stress disorder in rats
MicroRNA-101 regulates the viability and invasion of cervical cancer cells
Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) Downregulates the Expression of Protumor Factors Cyclooxygenase-2 and Inducible Nitric Oxide Synthase in a GM-CSF Receptor-Independent Manner in Cervical Cancer Cells
Odontogenic epithelial proliferation is correlated with COX-2 expression in dentigerous cyst and ameloblastoma
Anti-inflammatory Effects of Phyllanthus emblica L on Benzopyrene-Induced Precancerous Lung Lesion by Regulating the IL-1?/miR-101/Lin28B Signaling Pathway
miR-144 and targets, c-fos and cyclooxygenase-2 (COX2), modulate synthesis of PGE2 in the amnion during pregnancy and labor
Glycyrrhizic Acid Attenuates Sepsis-Induced Acute Kidney Injury by Inhibiting NF-?B Signaling Pathway
MicroRNA-27b Regulates Mitochondria Biogenesis in Myocytes
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Customer Q&As
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4 Customer Q&As for Anti-MUC1 Antibody
Question
My colleagues were content with the WB result of your anti-MUC1 antibody. However we have seen positive staining in mammary carcinoma isoform 9: secreted. using this antibody. Is that expected? Could you tell me where is MUC1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-04-21
Answer
From literature, mammary carcinoma does express MUC1. Generally MUC1 expresses in apical cell membrane, isoform 5: secreted., isoform y: secreted., isoform 9: secreted., mucin-1 subunit beta: cell membrane. Regarding which tissues have MUC1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 15969018
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Lung, Pubmed ID: 8604237
Mammary carcinoma, Pubmed ID: 1697589, 11341784
Pancreatic carcinoma, Pubmed ID: 2394722
Boster Scientific Support
Answered: 2020-04-21
Question
We have seen staining in human carcinoma. Do you have any suggestions? Is anti-MUC1 antibody supposed to stain carcinoma positively?
Verified Customer
Verified customer
Asked: 2020-02-10
Answer
From literature carcinoma does express MUC1. From Uniprot.org, MUC1 is expressed in body of stomach, pancreatic carcinoma, mammary carcinoma, carcinoma, cervix carcinoma, thyroid, lung, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have MUC1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 15969018
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Lung, Pubmed ID: 8604237
Mammary carcinoma, Pubmed ID: 1697589, 11341784
Pancreatic carcinoma, Pubmed ID: 2394722
Boster Scientific Support
Answered: 2020-02-10
Question
We are currently using anti-MUC1 antibody PB9145 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on feline tissues as well?
O. Huang
Verified customer
Asked: 2019-11-26
Answer
The anti-MUC1 antibody (PB9145) has not been validated for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-26
Question
I am interested in using your anti-MUC1 antibody for nicotinamide salvaging studies. Has this antibody been tested with western blotting on ovary cancer tissue? We would like to see some validation images before ordering.
R. Brown
Verified customer
Asked: 2015-07-28
Answer
I appreciate your inquiry. This PB9145 anti-MUC1 antibody is tested on ovary cancer tissue. It is guaranteed to work for IHC in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2015-07-28
