Product Info Summary
SKU: | PA1483 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IHC, IHC-F, WB |
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Product info
Product Name
Anti-MTA1 Antibody
SKU/Catalog Number
PA1483
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MTA1 Antibody catalog # PA1483. Tested in IHC, IHC-F, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MTA1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1483)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human MTA1, identical to the related mouse and rat sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1483 is reactive to MTA1 in Human, Mouse, Rat
Applications
PA1483 is guaranteed for IHC, IHC-F, WB Boster Guarantee
Observed Molecular Weight
80 kDa
Calculated molecular weight
80786 MW
Background of MTA1
Metastasis-associated protein MTA1 is a protein that in humans is encoded by the MTA1 gene. This gene encodes a protein that was identified in a screen for genes expressed in metastatic cells, specifically, mammary adenocarcinoma cell lines. Expression of this gene has been correlated with the metastatic potential of at least two types of carcinomas although it is also expressed in many normal tissues.By fluorescence in situ hybridization, mapped the MTA1gene to chromosome 14q32.3. MTA1 is a component of the chromatin remodeling complex that influences gene transcription by modulating target gene chromatin. MTA1 is widely upregulated in many carcinomas.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Mouse, Rat, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Validation Images & Assay Conditions
![pa1483 mta1 primary antibodies wb testing 1 pa1483 mta1 primary antibodies wb testing 1](https://www.bosterbio.com/media/catalog/product/p/a/pa1483-mta1-primary-antibodies-wb-testing-1.jpg)
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Figure 1. Western blot analysis of MTA1 using anti-MTA1 antibody (PA1483).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human HEK293 whole cell lysates,
Lane 3: human HELA whole cell lysates,
Lane 4: human PC-3 whole cell lysates,
Lane 5: human Raji whole cell lysates,
Lane 6: human K562 whole cell lysates,
Lane 7: rat brain tissue lysates,
Lane 8: mouse brain tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MTA1 antigen affinity purified polyclonal antibody (Catalog # PA1483) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MTA1 at approximately 80KD. The expected band size for MTA1 is at 80KD.
![pa1483 2 IHC anti mta1 antibody pa1483 2 IHC anti mta1 antibody](https://www.bosterbio.com/media/catalog/product/antibody/pa1483-2-IHC-anti-mta1-antibody.jpg)
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Anti-MTA1 antibody, PA1483, IHC(P)
IHC(P): Human Rectal Cancer Tissue
![pa1483 4_ pa1483 4_](https://www.bosterbio.com/media/catalog/product/p/a/pa1483-4_.jpg)
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Figure 4. IHC analysis of MTA1 using anti-MTA1 antibody (PA1483).
MTA1 was detected in paraffin-embedded section of rat ovary tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MTA1Antibody (PA1483) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
![pa1483 3 IHC anti mta1 antibody pa1483 3 IHC anti mta1 antibody](https://www.bosterbio.com/media/catalog/product/antibody/pa1483-3-IHC-anti-mta1-antibody.jpg)
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Anti-MTA1 antibody, PA1483, IHC(F)
IHC(F): Rat Ovary Tissue
![pa1483 5 pa1483 5](https://www.bosterbio.com/media/catalog/product/p/a/pa1483-5.jpg)
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Figure 5. IHC analysis of MTA1 using anti-MTA1 antibody (PA1483).
MTA1 was detected in paraffin-embedded section of rat ovary tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MTA1 Antibody (PA1483) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
![pa1483 6 pa1483 6](https://www.bosterbio.com/media/catalog/product/p/a/pa1483-6.jpg)
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Figure 6. IHC analysis of MTA1 using anti-MTA1 antibody (PA1483).
MTA1 was detected in frozen section of mouse brain tissues. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MTA1 Antibody (PA1483) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
![pa1483 mta1 primary antibodies ihc testing 7_1 pa1483 mta1 primary antibodies ihc testing 7_1](https://www.bosterbio.com/media/catalog/product/p/a/pa1483-mta1-primary-antibodies-ihc-testing-7_1.jpg)
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Figure 7. IHC analysis of MTA1 using anti-MTA1 antibody (PA1483).
MTA1 was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MTA1 Antibody (PA1483) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
![pa1483 mta1 primary antibodies ihc testing 8 pa1483 mta1 primary antibodies ihc testing 8](https://www.bosterbio.com/media/catalog/product/p/a/pa1483-mta1-primary-antibodies-ihc-testing-8.jpg)
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Figure 8. IHC analysis of MTA1 using anti-MTA1 antibody (PA1483).
MTA1 was detected in paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MTA1 Antibody (PA1483) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
![pa1483 mta1 primary antibodies ihc testing 9 pa1483 mta1 primary antibodies ihc testing 9](https://www.bosterbio.com/media/catalog/product/p/a/pa1483-mta1-primary-antibodies-ihc-testing-9.jpg)
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Figure 9. IHC analysis of MTA1 using anti-MTA1 antibody (PA1483).
MTA1 was detected in paraffin-embedded section of human pancrease cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MTA1 Antibody (PA1483) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For MTA1 (Source: Uniprot.org, NCBI)
Gene Name
MTA1
Full Name
Metastasis-associated protein MTA1
Weight
80786 MW
Alternative Names
metastasis associated 1; metastasis associated gene 1 protein; metastasis associated protein; metastasis-associated protein MTA1 MTA1 metastasis associated 1 metastasis-associated protein MTA1|metastasis associated gene 1 protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MTA1, check out the MTA1 Infographic
![MTA1 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MTA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MTA1 Antibody (PA1483)
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1 Customer Q&As for Anti-MTA1 Antibody
Question
We are currently using anti-MTA1 antibody PA1483 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-18
Answer
The anti-MTA1 antibody (PA1483) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-18