Anti-High mobility group protein B4 HMGB4 Antibody Picoband®

Product Name

Anti-High mobility group protein B4 HMGB4 Antibody Picoband®

View all Hmgb4 Antibodies

SKU/Catalog Number

PA1042

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-High mobility group protein B4 HMGB4 Antibody catalog # PA1042. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-High mobility group protein B4 HMGB4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1042)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human HMGB4, different from the related mouse and rat sequences by one amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1042 is reactive to HMGB4 in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

23 kDa

Calculated molecular weight

22490 MW

Background of Hmgb4

HMGB4 is HMG2 like, isoform 2, high-mobility group box 4. This gene can be found on Chromosome 1. HMGB4 contains two HMG-box regions, which is found in a variety of eukaryotic chromosomal proteins and transcription. HMGB proteins are phosphorylated to various extents reveals that the existence of differentially modified forms increases the number of distinct HMGB protein variants in plant chromatin that may be adapted to certain functions.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PA1042 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse

Positive Control

WB: Rat Brain Tissue, Rat Testis Tissue, JURKAT Cell, HT1080 Cell

Validation Images & Assay Conditions

Gene/Protein Information For HMGB4 (Source: Uniprot.org, NCBI)

Alternative Names

High mobility group protein B4;HMGB4; Hmgb4|1700001F22Rik, AI326135|high-mobility group box 4|high mobility group protein B4

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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