Anti-MSK2 (Ab-568) RPS6KA4 Antibody

Product Name

Anti-MSK2 (Ab-568) RPS6KA4 Antibody

View all MSK2 Antibodies

SKU/Catalog Number

A05545

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-MSK2 (Ab-568) RPS6KA4 Antibody (Catalog # A05545). Tested in WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MSK2 (Ab-568) RPS6KA4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05545)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized non-phosphopeptide derived from human MSK2 around the phosphorylation site of threonine 568 (M-Q-T(p)-P-C).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A05545 is reactive to RPS6KA4 in Human, Mouse

Reconstitution

Observed Molecular Weight

111 kDa

Calculated molecular weight

85606 MW

Background of MSK2

Serine/threonine-protein kinase that is required for the mitogen or stress-induced phosphorylation of the transcription factors CREB1 and ATF1 and for the regulation of the transcription factor RELA, and that contributes to gene activation by histone phosphorylation and functions in the regulation of inflammatory genes. Phosphorylates CREB1 and ATF1 in response to mitogenic or stress stimuli such as UV-C irradiation, epidermal growth factor (EGF) and anisomycin. Plays an essential role in the control of RELA transcriptional activity in response to TNF. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and EGF, which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. May also phosphorylate 'Ser-28' of histone H3. Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 1 (HMGN1/HMG14). In lipopolysaccharide-stimulated primary macrophages, acts downstream of the Toll-like receptor TLR4 to limit the production of pro-inflammatory cytokines. Functions probably by inducing transcription of the MAP kinase phosphatase DUSP1 and the anti-inflammatory cytokine interleukin 10 (IL10), via CREB1 and ATF1 transcription factors.

Pierrat B.,J. Biol. Chem. 273:29661-29671(1998).
Deak M.,EMBO J. 17:4426-4441(1998).
Soloaga A.,EMBO J. 22:2788-2797(2003).<

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A05545 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000

Validation Images & Assay Conditions

Gene/Protein Information For RPS6KA4 (Source: Uniprot.org, NCBI)

Alternative Names

Ribosomal protein S6 kinase alpha-4;S6K-alpha-4;2.7.11.1;90 kDa ribosomal protein S6 kinase 4;Nuclear mitogen- and stress-activated protein kinase 2;Ribosomal protein kinase B;RSKB;RPS6KA4;MSK2; RPS6KA4 MSK2, RSK-B, S6K-alpha-4 ribosomal protein S6 kinase A4 ribosomal protein S6 kinase alpha-4|90 kDa ribosomal protein S6 kinase 4|mitogen- and stress-activated protein kinase 2|nuclear mitogen- and stress-activated protein kinase 2|ribosomal protein S6 kinase, 90kDa, polypeptide 4|ribosomal protein kinase B

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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