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Product Info Summary
| SKU: | A07454 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-MRCKB CDC42BPB Antibody
SKU/Catalog Number
A07454
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-MRCKB CDC42BPB Antibody (Catalog # A07454). Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MRCKB CDC42BPB Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07454)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human MRCKB.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A07454 is reactive to CDC42BPB in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
194315 MW
Background of CDC42BPB
Serine/threonine-protein kinase which is an important downstream effector of CDC42 and plays a role in the regulation of cytoskeleton reorganization and cell migration. Regulates actin cytoskeletal reorganization via phosphorylation of PPP1R12C and MYL9/MLC2. In concert with MYO18A and LURAP1, is involved in modulating lamellar actomyosin retrograde flow that is crucial to cell protrusion and migration. Phosphorylates PPP1R12A.
Nakajima D., DNA Res. 9:99-106(2002).
Olsen J.V., Cell 127:635-648(2006).
Hirosawa M., DNA Res. 6:329-336(1999).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07454 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Validation Images & Assay Conditions
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Western blot analysis of extracts from COLO cells, using MRCKB antibody A07454.
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Immunohistochemistry analysis of paraffin-embedded human brain tissue using MRCKB antibody A07454.
Protein Target Info & Infographic
Gene/Protein Information For CDC42BPB (Source: Uniprot.org, NCBI)
Gene Name
CDC42BPB
Full Name
Serine/threonine-protein kinase MRCK beta
Weight
194315 MW
Superfamily
protein kinase superfamily
Alternative Names
Serine/threonine-protein kinase MRCK beta;2.7.11.1;CDC42-binding protein kinase beta;CDC42BP-beta;DMPK-like beta;Myotonic dystrophy kinase-related CDC42-binding kinase beta;MRCK beta;Myotonic dystrophy protein kinase-like beta;CDC42BPB ;KIAA1124 ; CDC42BPB MRCKB CDC42 binding protein kinase beta serine/threonine-protein kinase MRCK beta|CDC42 binding protein kinase beta (DMPK-like)|CDC42BP-beta|DMPK-like beta|MRCK beta|myotonic dystrophy kinase-related CDC42-binding kinase beta|myotonic dystrophy protein kinase-like beta
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CDC42BPB, check out the CDC42BPB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CDC42BPB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MRCKB CDC42BPB Antibody (A07454)
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No publications found for A07454
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Customer Q&As
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5 Customer Q&As for Anti-MRCKB CDC42BPB Antibody
Question
We are currently using anti-MRCKB antibody A07454 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-13
Answer
The anti-MRCKB antibody (A07454) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-13
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain {eco:0000312 using anti-MRCKB antibody A07454. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-05-13
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-13
Question
I was wanting to use your anti-MRCKB antibody for IHC for mouse brain eco:0000312 on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been validated and is this antibody a good choice for mouse brain {eco:0000312 identification?
S. Mitchell
Verified customer
Asked: 2016-11-17
Answer
As indicated on the product datasheet, A07454 anti-MRCKB antibody has been tested for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse brain {eco:0000312 in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-11-17
Question
See attached the WB image, lot number and protocol we used for brain {eco:0000312 using anti-MRCKB antibody A07454. Please let me know if you require anything else.
G. Huang
Verified customer
Asked: 2016-09-21
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-09-21
Question
My question regards to test anti-MRCKB antibody A07454 on mouse brain {eco:0000312 for research purposes, then I may be interested in using anti-MRCKB antibody A07454 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
D. Li
Verified customer
Asked: 2014-03-06
Answer
The products we sell, including anti-MRCKB antibody A07454, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2014-03-06
