Product Info Summary
SKU: | A06427 |
---|---|
Size: | 100uL |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, WB |
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Product info
Product Name
Anti-Protein NEDD1 NEDD1 Antibody
SKU/Catalog Number
A06427
Size
100uL
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-Protein NEDD1 NEDD1 Antibody (Catalog # A06427). Tested in ELISA, WB applications. This antibody reacts with Human.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-Protein NEDD1 NEDD1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06427)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
Antiserum
Immunogen
Anti-NEDD1 was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to the 343-667 region of human Nedd1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A06427 is reactive to Nedd1 in Human
Applications
A06427 is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
79710 MW
Background of Nedd1
This antibody is suitable for Cancer, Immunology and Nuclear Signaling research. Microtubules are polymers of tubulin, which exist as heterodimers of alpha-tubulin and beta-tubulin. NEDD1 (neural precursor expressed, developmentally down-regulated protein1; also called GCP-WD) is a centrosomal protein that in mammals associates with the gamma-tubulin ring complex. Gamma-TuRC is critical for initiation, or nucleation, of the microtubule assembly. In association with this complex, NEDD1 plays an important role in targeting the gamma-TuRC complex to the site of microtubule nucleation and to the mitotic spindle. These events are essential for proper bipolar spindle formation and mitotic progression. Given the casual link between improper spindle function and tumorigenesis, characterization of Nedd1 function will be important to better understand various mechanisms underlying mitotic regulation, chromosome segregation, and cancer development.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:100,000
WB: 1:10,000
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of NEDD1 using anti-NEDD1 antibody (A06427).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NEDD1 antigen affinity purified polyclonal antibody (Catalog # A06427) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for NEDD1.
Protein Target Info & Infographic
Gene/Protein Information For Nedd1 (Source: Uniprot.org, NCBI)
Gene Name
Nedd1
Full Name
Protein NEDD1
Weight
79710 MW
Alternative Names
FLJ35902; GCP-WD; NEDD-1; Neural precursor cell expressed developmentally down-regulated protein 1; neural precursor cell expressed, developmentally down-regulated 1; protein NEDD1; TUBGCP7 Nedd1|neural precursor cell expressed, developmentally down-regulated gene 1|protein NEDD1|NEDD-1|neural precursor cell expressed developmentally down-regulated protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Nedd1, check out the Nedd1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Nedd1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Protein NEDD1 NEDD1 Antibody (A06427)
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Customer Q&As
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1 Customer Q&As for Anti-Protein NEDD1 NEDD1 Antibody
Question
We are currently using anti-NEDD1 antibody A06427 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-03
Answer
The anti-NEDD1 antibody (A06427) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-03