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Product Info Summary
| SKU: | PB10079 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-MPP2 Antibody Picoband®
SKU/Catalog Number
PB10079
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MPP2 Antibody Picoband® catalog # PB10079. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MPP2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10079)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human MPP2 recombinant protein (Position: R387-Y576). Human MPP2 shares 96.8% amino acid (aa) sequence identity with mouse MPP2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB10079 is reactive to MPP2 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
65 kDa
Calculated molecular weight
64581 MW
Background of MPP2
MAGUK p55 subfamily member 2 is a protein that in humans is encoded by the MPP2 gene. Palmitoylated membrane protein 2 is a member of a family of membrane-associated proteins termed MAGUKs (membrane-associated guanylate kinase homologs). MAGUKs interact with the cytoskeleton and regulate cell proliferation, signaling pathways, and intracellular junctions. Palmitoylated membrane protein 2 contains a conserved sequence, called the SH3 (src homology 3) motif, found in several other proteins that associate with the cytoskeleton and are suspected to play important roles in signal transduction.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB10079 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: rat brain tissue, mouse brain tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of MPP2 using anti-MPP2 antibody (PB10079).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MPP2 antigen affinity purified polyclonal antibody (Catalog # PB10079) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MPP2 at approximately 65 kDa. The expected band size for MPP2 is at 65 kDa.
Protein Target Info & Infographic
Gene/Protein Information For MPP2 (Source: Uniprot.org, NCBI)
Gene Name
MPP2
Full Name
MAGUK p55 subfamily member 2
Weight
64581 MW
Superfamily
MAGUK family
Alternative Names
MAGUK p55 subfamily member 2;Discs large homolog 2;Protein MPP2;MPP2;DLG2; MPP2 DLG2 membrane palmitoylated protein 2 MAGUK p55 subfamily member 2|discs large, homolog 2|membrane protein, palmitoylated 2 (MAGUK p55 subfamily member 2)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MPP2, check out the MPP2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MPP2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MPP2 Antibody Picoband® (PB10079)
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Customer Q&As
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7 Customer Q&As for Anti-MPP2 Antibody Picoband®
Question
See below the WB image, lot number and protocol we used for amygdala hippocampus using anti-MPP2 antibody PB10079. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-11-18
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-18
Question
We are currently using anti-MPP2 antibody PB10079 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-30
Answer
The anti-MPP2 antibody (PB10079) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-30
Question
Does anti-MPP2 antibody PB10079 work for Flow Cytometry with amygdala hippocampus?
Verified Customer
Verified customer
Asked: 2019-07-16
Answer
According to the expression profile of amygdala hippocampus, MPP2 is highly expressed in amygdala hippocampus. So, it is likely that anti-MPP2 antibody PB10079 will work for Flow Cytometry with amygdala hippocampus.
Boster Scientific Support
Answered: 2019-07-16
Question
I was wanting to use your anti-MPP2 antibody for Flow Cytometry for rat amygdala hippocampus on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat amygdala hippocampus identification?
Verified Customer
Verified customer
Asked: 2019-06-26
Answer
As indicated on the product datasheet, PB10079 anti-MPP2 antibody has been validated for Flow Cytometry, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat amygdala hippocampus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-26
Question
Is a blocking peptide available for product anti-MPP2 antibody (PB10079)?
Verified Customer
Verified customer
Asked: 2018-12-17
Answer
We do provide the blocking peptide for product anti-MPP2 antibody (PB10079). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-12-17
Question
Is this PB10079 anti-MPP2 antibody reactive to the isotypes of MPP2?
W. Patel
Verified customer
Asked: 2018-04-19
Answer
The immunogen of PB10079 anti-MPP2 antibody is E. coli-derived human MPP2 recombinant protein (Position: R387-Y576). Human MPP2 shares 96.8% amino acid (aa) sequence identity with mouse MPP2. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-04-19
Question
Does PB10079 anti-MPP2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
E. Mitchell
Verified customer
Asked: 2014-03-14
Answer
It shows on the product datasheet, PB10079 anti-MPP2 antibody as been tested on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2014-03-14
