Product Info Summary
SKU: | A04952 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-MOV10 Antibody Picoband™
SKU/Catalog Number
A04952
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MOV10 Antibody Picoband™ catalog # A04952. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-MOV10 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04952)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human MOV10 recombinant protein (Position: H167-Q772).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A04952 is reactive to MOV10 in Human
Applications
A04952 is guaranteed for ELISA, Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
114 kDa
Calculated molecular weight
45468 MW
Background of MOV10
Putative helicase MOV-10 is an enzyme that in humans is encoded by the MOV10 gene.MOV10, also named as KIAA1631, belongs to the DNA2/NAM7 helicase family and SDE3 subfamily. It is required for RNA-mediated gene silencing by the RNA-induced silencing complex (RISC). HumanMOV10may regulate a wide range of RNA viruses and could also control the retrotransposition of endogenous retroelements in mammals. MOV10 has a broad antiretroviral activity that can target a wide range of retroviruses, and it could be actively involved in host defense against retroviral infection.MOV10 can potently inhibit HIV-1 replication at multiple stages. It is involved in the progression of telomerase-catalyzing reaction via the interaction of telomerase protein and telomere DNA.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
Direct ELISA, 0.1-0.5 μg/ml, Human
Validation Images & Assay Conditions
![a04952 mov10 primary antibodies wb testing 1 a04952 mov10 primary antibodies wb testing 1](https://www.bosterbio.com/media/catalog/product/a/0/a04952-mov10-primary-antibodies-wb-testing-1.jpg)
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Figure 1. Western blot analysis of MOV10 using anti-MOV10 antibody (A04952).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human Jurkat whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MOV10 antigen affinity purified polyclonal antibody (Catalog # A04952) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MOV10 at approximately 114 kDa. The expected band size for MOV10 is at 114 kDa.
![a04952 mov10 primary antibodies ihc testing 2 a04952 mov10 primary antibodies ihc testing 2](https://www.bosterbio.com/media/catalog/product/a/0/a04952-mov10-primary-antibodies-ihc-testing-2.jpg)
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Figure 2. IHC analysis of MOV10 using anti-MOV10 antibody (A04952).
MOV10 was detected in a paraffin-embedded section of human appendiceal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MOV10 Antibody (A04952) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
![a04952 mov10 primary antibodies ihc testing 3 a04952 mov10 primary antibodies ihc testing 3](https://www.bosterbio.com/media/catalog/product/a/0/a04952-mov10-primary-antibodies-ihc-testing-3.jpg)
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Figure 3. IHC analysis of MOV10 using anti-MOV10 antibody (A04952).
MOV10 was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MOV10 Antibody (A04952) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
![a04952 mov10 primary antibodies ihc testing 4 a04952 mov10 primary antibodies ihc testing 4](https://www.bosterbio.com/media/catalog/product/a/0/a04952-mov10-primary-antibodies-ihc-testing-4.jpg)
Click image to see more details
Figure 4. IHC analysis of MOV10 using anti-MOV10 antibody (A04952).
MOV10 was detected in a paraffin-embedded section of human gall bladder adenosquamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MOV10 Antibody (A04952) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
![a04952 mov10 primary antibodies ihc testing 5 a04952 mov10 primary antibodies ihc testing 5](https://www.bosterbio.com/media/catalog/product/a/0/a04952-mov10-primary-antibodies-ihc-testing-5.jpg)
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Figure 5. IHC analysis of MOV10 using anti-MOV10 antibody (A04952).
MOV10 was detected in a paraffin-embedded section of human gastric adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MOV10 Antibody (A04952) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
![a04952 mov10 primary antibodies ihc testing 6 a04952 mov10 primary antibodies ihc testing 6](https://www.bosterbio.com/media/catalog/product/a/0/a04952-mov10-primary-antibodies-ihc-testing-6.jpg)
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Figure 6. IHC analysis of MOV10 using anti-MOV10 antibody (A04952).
MOV10 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MOV10 Antibody (A04952) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
![a04952 mov10 primary antibodies if testing 7 a04952 mov10 primary antibodies if testing 7](https://www.bosterbio.com/media/catalog/product/a/0/a04952-mov10-primary-antibodies-if-testing-7.jpg)
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Figure 7. IF analysis of MOV10 using anti-MOV10 antibody (A04952).
MOV10 was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-MOV10 Antibody (A04952) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
![a04952 mov10 primary antibodies fcm testing 8 a04952 mov10 primary antibodies fcm testing 8](https://www.bosterbio.com/media/catalog/product/a/0/a04952-mov10-primary-antibodies-fcm-testing-8.png)
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Figure 8. Flow Cytometry analysis of Hela cells using anti-MOV10 antibody (A04952).
Overlay histogram showing Hela cells stained with A04952 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MOV10 Antibody (A04952, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For MOV10 (Source: Uniprot.org, NCBI)
Gene Name
MOV10
Full Name
Helicase MOV-10
Weight
45468 MW
Superfamily
DNA2/NAM7 helicase family
Alternative Names
DKFZp667O1423; EC 3.6.1; EC 3.6.4.13; FLJ32791; KIAA1631mouse) homolog; Mov10, Moloney leukemia virus 10, homolog (mouse) MOV10 fSAP113, gb110 Mov10 RISC complex RNA helicase helicase MOV-10|Mov10, Moloney leukemia virus 10, homolog|armitage homolog|functional spliceosome-associated protein 113|moloney leukemia virus 10 protein|putative helicase MOV-10
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MOV10, check out the MOV10 Infographic
![MOV10 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MOV10: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MOV10 Antibody Picoband™ (A04952)
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