Click image to see more details
Product Info Summary
| SKU: | A07912-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-GPD1L Antibody Picoband®
SKU/Catalog Number
A07912-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-GPD1L Antibody Picoband® catalog # A07912-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GPD1L Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07912-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human GPD1L recombinant protein (Position: A19-T351).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A07912-1 is reactive to GPD1L in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
38 kDa
Calculated molecular weight
49229 MW
Background of GPD1L
GPD1L is a human gene. It is mapped to 3p22.3. The protein encoded by this gene contains a glycerol-3-phosphate dehydrogenase (NAD+) motif and shares 72% sequence identity with GPD1. The encoded protein is found in the cytoplasm, associated with the plasma membrane, where it binds the sodium channel, voltage-gated, type V, alpha subunit (SCN5A). Defects in this gene are a cause of Brugada syndrome type 2 (BRS2) as well as sudden infant death syndrome (SIDS).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07912-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: human placenta issue, human PC-3 whole cell, human U-87MG whole cell, human HL-60 whole cell, human HepG2 whole cell, human A549 whole cell, human Caco-2 whole cell, human K562 whole cell, rat heart issue, mouse RAW2467 whole cell
Validation Images & Assay Conditions
Click image to see more details
Boster Kit Box
Click image to see more details
Figure 1. Western blot analysis of GPD1L using anti-GPD1L antibody (A07912-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human placenta issue lysates,
Lane 2: human PC-3 whole cell lysates,
Lane 3: human U-87MG whole cell lysates,
Lane 4: human HL-60 whole cell lysates,
Lane 5: human HepG2 whole cell lysates,
Lane 6: human A549 whole cell lysates,
Lane 7: human Caco-2 whole cell lysates,
Lane 8: human K562 whole cell lysates,
Lane 9: rat heart issue lysates,
Lane 10: mouse RAW246.7 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GPD1L antigen affinity purified polyclonal antibody (Catalog # A07912-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GPD1L at approximately 38KD. The expected band size for GPD1L is at 38KD.
Protein Target Info & Infographic
Gene/Protein Information For GPD1L (Source: Uniprot.org, NCBI)
Gene Name
GPD1L
Full Name
Glycerol-3-phosphate dehydrogenase 1-like protein
Weight
49229 MW
Superfamily
NAD-dependent glycerol-3-phosphate dehydrogenase family
Alternative Names
Glycerol-3-phosphate dehydrogenase 1-like protein; GPD1-L; GPD1L; KIAA0089 GPD1L GPD1-L glycerol-3-phosphate dehydrogenase 1 like glycerol-3-phosphate dehydrogenase 1-like protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GPD1L, check out the GPD1L Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GPD1L: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GPD1L Antibody Picoband® (A07912-1)
Hello CJ!
No publications found for A07912-1
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-GPD1L Antibody Picoband®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-GPD1L Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
5 Customer Q&As for Anti-GPD1L Antibody Picoband®
Question
We are currently using anti-GPD1L antibody A07912-1 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-27
Answer
The anti-GPD1L antibody (A07912-1) has not been tested for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-27
Question
Does anti-GPD1L antibody A07912-1 work on dog WB with brain placenta?
Verified Customer
Verified customer
Asked: 2020-02-27
Answer
Our lab technicians have not tested anti-GPD1L antibody A07912-1 on dog. You can run a BLAST between dog and the immunogen sequence of anti-GPD1L antibody A07912-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog brain placenta in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-27
Question
Is a blocking peptide available for product anti-GPD1L antibody (A07912-1)?
Verified Customer
Verified customer
Asked: 2020-02-19
Answer
We do provide the blocking peptide for product anti-GPD1L antibody (A07912-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-02-19
Question
Will anti-GPD1L antibody A07912-1 work for ELISA with myocardium?
Verified Customer
Verified customer
Asked: 2019-06-10
Answer
According to the expression profile of myocardium, GPD1L is highly expressed in myocardium. So, it is likely that anti-GPD1L antibody A07912-1 will work for ELISA with myocardium.
Boster Scientific Support
Answered: 2019-06-10
Question
I was wanting to use your anti-GPD1L antibody for ELISA for mouse myocardium on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse myocardium identification?
L. Zhang
Verified customer
Asked: 2014-01-22
Answer
As indicated on the product datasheet, A07912-1 anti-GPD1L antibody has been tested for ELISA, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse myocardium in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-01-22
