Anti-(Mouse) Uhrf1 Antibody (C-term)

Product Name

Anti-(Mouse) Uhrf1 Antibody (C-term)

View all Uhrf1 Antibodies

SKU/Catalog Number

M01156-1

Size

80 µl

Description

Boster Bio Anti-(Mouse) Uhrf1 Antibody (C-term) (Catalog # M01156-1). Tested in WB application(s). This antibody reacts with Mouse.

Storage & Handling

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Cite This Product

Anti-(Mouse) Uhrf1 Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01156-1)

Host

Rabbit

Contents

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

This Mouse Uhrf1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 596-628 amino acids from the C-terminal region of Mouse Uhrf1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M01156-1 is reactive to Uhrf1 in Mouse

Applications

M01156-1 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

88304 Da

Background of Uhrf1

Multidomain protein that acts as a key epigenetic regulator by bridging DNA methylation and chromatin modification. Specifically recognizes and binds hemimethylated DNA at replication forks via its YDG domain and recruits DNMT1 methyltransferase to ensure faithful propagation of the DNA methylation patterns through DNA replication. In addition to its role in maintenance of DNA methylation, also plays a key role in chromatin modification: through its tudor-like regions and PHD- type zinc fingers, specifically recognizes and binds histone H3 trimethylated at 'Lys-9' (H3K9me3) and unmethylated at 'Arg-2' (H3R2me0), respectively, and recruits chromatin proteins. Enriched in pericentric heterochromatin where it recruits different chromatin modifiers required for this chromatin replication. Also localizes to euchromatic regions where it negatively regulates transcription possibly by impacting DNA methylation and histone modifications. Has E3 ubiquitin-protein ligase activity by mediating the ubiquitination of target proteins such as histone H3 and PML. It is still unclear how E3 ubiquitin-protein ligase activity is related to its role in chromatin in vivo. May be involved in DNA repair.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:2000

Validation Images & Assay Conditions

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Anti-Uhrf1 Antibody (C-term) at 1:1000 dilution + mouse heart lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 88 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.

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Anti-Uhrf1 Antibody (C-term) at 1:2000 dilution + mouse thymus lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 88 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.

Gene/Protein Information For Uhrf1 (Source: Uniprot.org, NCBI)

Alternative Names

E3 ubiquitin-protein ligase UHRF1; EC 6.3.2; EC 6.3.2.-; FLJ21925; hNP95; huNp95; ICBP90NP95; Inverted CCAAT box-binding protein of 90 kDa; Np95; Nuclear protein 95; Nuclear zinc finger protein Np95; RING finger protein 106; RNF106MGC138707; Transcription factor ICBP90; Ubiquitin-like PHD and RING finger domain-containing protein 1; ubiquitin-like with PHD and ring finger domains 1; ubiquitin-like, containing PHD and RING finger domains, 1; Ubiquitin-like-containing PHD and RING finger domains protein 1 Uhrf1|AL022808, ICBP, ICBP90, Np, Np95, RNF106|ubiquitin-like, containing PHD and RING finger domains, 1|E3 ubiquitin-protein ligase UHRF1|RING-type E3 ubiquitin transferase UHRF1|mUhrf1|nuclear protein 95|nuclear zinc finger protein Np95|ubiquitin-like PHD and RING finger domain-containing protein 1|ubiquitin-like-containing PHD and RING finger domains protein 1

For more info on Uhrf1, check out the Uhrf1 Infographic

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In this infographic, you will see the following information for Uhrf1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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