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Product Info Summary
| SKU: | M00172-1 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human, Mouse, Zebrafish, Fish |
| Host: | Mouse |
| Application: | ChIP, IP, IHC, WB |
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Product info
Product Name
Anti-DNMT1 Monoclonal Antibody
SKU/Catalog Number
M00172-1
Size
100ug
Form
liquid
Description
Boster Bio Anti-DNMT1 Monoclonal Antibody catalog # M00172-1. Tested in ChIP, IP, IHC, WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DNMT1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00172-1)
Host
Mouse
Contents
PBS, 50% glycerol, 0.09% sodium azide
Clonality
Monoclonal
Clone Number
60B1220.1
Isotype
IgG1 Kappa
Immunogen
Raised against a synthetic peptide corresponding to amino acids 637-650 of human DNMT1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Detects ~180kDa. It will cross-react with mouse DNMT1.
Reactive Species
M00172-1 is reactive to DNMT1 in Human, Mouse, Zebrafish, Fish
Reconstitution
Observed Molecular Weight
42 kDa
Calculated molecular weight
183.165kDa
Background of DNMT1
Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Dnmt1 co-purifies with the retinoblastoma (Rb) tumour suppressor gene product, E2F1, and HDAC1. Dnmt1 also cooperates with Rb to repress transcription from promoters containing E2F-binding sites suggesting a link between DNA methylation, histone deacetylase and sequence-specific DNA binding activity, as well as a growth-regulatory pathway that is disrupted in nearly all cancer cells (1-6).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00172-1 is guaranteed for ChIP, IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB (1:1000), IHC (1:1000); optimal dilutions for assays should be determined by the user.
Validation Images & Assay Conditions
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Figure 1. IHC analysis of DNMT1 using anti-DNMT1 antibody (M00172-1).
DNMT1 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-DNMT1 Antibody (M00172-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
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Figure 2. Western blot analysis of DNMT1 using anti-DNMT1 antibody (M00172-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DNMT1 antigen affinity purified polyclonal antibody (Catalog # M00172-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system.
Click image to see more details
Figure 3. IHC analysis of DNMT1 using anti-DNMT1 antibody (M00172-1).
DNMT1 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-DNMT1 Antibody (M00172-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For DNMT1 (Source: Uniprot.org, NCBI)
Gene Name
DNMT1
Full Name
DNA (cytosine-5)-methyltransferase 1
Weight
183.165kDa
Superfamily
class I-like SAM-binding methyltransferase superfamily
Alternative Names
DNA Mtase, DNMT, MCMT, DNA methyltransferase 1, AIM, CXXC9, DNMT, DNA (cytosine-5)-methyltransferase 1, CXXC-type zinc finger protein 9, DNA methyltransferase HsaI DNMT1 ADCADN, AIM, CXXC9, DNMT, HSN1E, MCMT, m.HsaI DNA methyltransferase 1 DNA (cytosine-5)-methyltransferase 1|CXXC-type zinc finger protein 9|DNA (cytosine-5-)-methyltransferase 1|DNA MTase HsaI|DNA methyltransferase HsaI
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DNMT1, check out the DNMT1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DNMT1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-DNMT1 Monoclonal Antibody (M00172-1)
Hello CJ!
M00172-1 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Zhang D,An X,Li Q,Man X,Chu M,Li H,Zhang N,Dai X,Yu H,Li Z.Thioguanine Induces Apoptosis in Triple-Negative Breast Cancer by Regulating PI3K-AKT Pathway.Front Oncol.2020 Oct 30;10:524922.doi:10.3389/fonc.2020.524922.PMID:33194583;PMCID:PMC7662440.
Species: Human, Mouse
M00172-1 usage in article: APP:IHC, SAMPLE:LIVER AND KIDNEY TISSUE, DILUTION:NA
Shikonin Inhibites Migration and Invasion of Thyroid Cancer Cells by Downregulating DNMT1
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-DNMT1 Monoclonal Antibody
Question
We purchased anti-DNMT1 Monoclonal antibody for IP on leukemic t-cell in the past. I am using mouse, and We are going to use the antibody for ChIP next. you antibody examining leukemic t-cell as well as cervix carcinoma in our next experiment. Could you please give me some suggestion on which antibody would work the best for ChIP?
Verified Customer
Verified customer
Asked: 2020-05-04
Answer
I took a look at the website and datasheets of our anti-DNMT1 Monoclonal antibody and it seems that M00172-1 has been validated on mouse in both IP and ChIP. Thus M00172-1 should work for your application. Our Boster satisfaction guarantee will cover this product for ChIP in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for ChIP detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-05-04
Question
Does anti-DNMT1 Monoclonal antibody M00172-1 work for IP with liver?
M. Collins
Verified customer
Asked: 2020-04-06
Answer
According to the expression profile of liver, DNMT1 is highly expressed in liver. So, it is likely that anti-DNMT1 Monoclonal antibody M00172-1 will work for IP with liver.
Boster Scientific Support
Answered: 2020-04-06
Question
Our lab were content with the WB result of your anti-DNMT1 Monoclonal antibody. However we have observed positive staining in cervix carcinoma nucleus. using this antibody. Is that expected? Could you tell me where is DNMT1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-10-09
Answer
From what I have seen in literature, cervix carcinoma does express DNMT1. Generally DNMT1 expresses in nucleus. Regarding which tissues have DNMT1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18220336, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Prostatic carcinoma, Pubmed ID: 15057824
Boster Scientific Support
Answered: 2019-10-09
Question
I was wanting to use your anti-DNMT1 Monoclonal antibody for IP for human liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human liver identification?
Verified Customer
Verified customer
Asked: 2019-08-28
Answer
It shows on the product datasheet, M00172-1 anti-DNMT1 Monoclonal antibody has been tested for ChIP, IP, IHC, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-28
Question
Is this M00172-1 anti-DNMT1 Monoclonal antibody reactive to the isotypes of DNMT1?
Verified Customer
Verified customer
Asked: 2019-08-15
Answer
The immunogen of M00172-1 anti-DNMT1 Monoclonal antibody is Raised against a synthetic peptide corresponding to amino acids 637-650 of human DNMT1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-08-15
Question
Will M00172-1 anti-DNMT1 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-06-21
Answer
As indicated on the product datasheet, M00172-1 anti-DNMT1 Monoclonal antibody as been validated on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-06-21
Question
I see that the anti-DNMT1 Monoclonal antibody M00172-1 works with IP, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-06-20
Answer
You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-06-20
Question
Would anti-DNMT1 Monoclonal antibody M00172-1 work on dog WB with prostatic carcinoma?
Verified Customer
Verified customer
Asked: 2019-06-20
Answer
Our lab technicians have not validated anti-DNMT1 Monoclonal antibody M00172-1 on dog. You can run a BLAST between dog and the immunogen sequence of anti-DNMT1 Monoclonal antibody M00172-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog prostatic carcinoma in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-20
Question
Is a blocking peptide available for product anti-DNMT1 Monoclonal antibody (M00172-1)?
Verified Customer
Verified customer
Asked: 2019-04-10
Answer
We do provide the blocking peptide for product anti-DNMT1 Monoclonal antibody (M00172-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-04-10
Question
We are currently using anti-DNMT1 Monoclonal antibody M00172-1 for mouse tissue, and we are content with the ChIP results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on feline tissues as well?
A. Mangal
Verified customer
Asked: 2018-05-08
Answer
The anti-DNMT1 Monoclonal antibody (M00172-1) has not been validated for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-05-08
Question
We have been able to see staining in mouse prostatic carcinoma. What should we do? Is anti-DNMT1 Monoclonal antibody supposed to stain prostatic carcinoma positively?
Verified Customer
Verified customer
Asked: 2017-12-12
Answer
From literature prostatic carcinoma does express DNMT1. From Uniprot.org, DNMT1 is expressed in oocyte, prostatic carcinoma, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have DNMT1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18220336, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Prostatic carcinoma, Pubmed ID: 15057824
Boster Scientific Support
Answered: 2017-12-12
Question
Do you have a BSA free version of anti-DNMT1 Monoclonal antibody M00172-1 available?
Verified Customer
Verified customer
Asked: 2017-11-03
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-DNMT1 Monoclonal antibody M00172-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-11-03
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-DNMT1 Monoclonal antibody M00172-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-07-27
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-07-27
Question
I am interested in to test anti-DNMT1 Monoclonal antibody M00172-1 on human liver for research purposes, then I may be interested in using anti-DNMT1 Monoclonal antibody M00172-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-06-15
Answer
The products we sell, including anti-DNMT1 Monoclonal antibody M00172-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-06-15
Question
Can you help my question with product M00172-1, anti-DNMT1 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
F. Singh
Verified customer
Asked: 2017-01-09
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00172-1 anti-DNMT1 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-01-09
Question
See below the WB image, lot number and protocol we used for liver using anti-DNMT1 Monoclonal antibody M00172-1. Please let me know if you require anything else.
M. Dhar
Verified customer
Asked: 2014-10-09
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-10-09
