Anti-Moesin Monoclonal Antibody

Product Name

Anti-Moesin Monoclonal Antibody

View all Moesin Antibodies

SKU/Catalog Number

M00766

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-Moesin Monoclonal Antibody (Catalog # M00766). Tested in Flow Cytometry, IF, WB, IHC applications. This antibody reacts with Human.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-Moesin Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00766)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: MSN/493

Isotype

IgG1, kappa

Immunogen

Recombinant full-length human Moesin protein

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M00766 is reactive to MSN in Human

Applications

M00766 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

67820 MW

Background of Moesin

Recognizes 78kDa moesin protein. Moesin, a member of the talin-4.1 superfamily, is a linking protein of the sub-membranous actin cytoskeleton. It is expressed in variable amounts in cells of different phenotypes such as macrophages, lymphocytes, fibroblastic, endothelial, epithelial, and neuronal cell lines but not in blood cells. The ERM proteins, ezrin, radixin, and moesin are involved in a variety of cellular functions, such as cell adhesion, migration, and the organization of cell surface structures, and are highly homologous, both in protein sequence and in functional activity, with merlin/schwannomin, a neurofibromatosis-2-associated tumor-suppressor protein. Cell lines of epithelial and mesothelial origin contain both moesin and radixin whereas cells of endothelial and lymphoid origin express moesin.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Reconstitution

Reconstitute with distilled water.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (2-4ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

Click image to see more details

Formalin-fixed, paraffin-embedded human Melanoma stained with Moesin Mouse Monoclonal Antibody (MSN/493).

Click image to see more details

Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with Moesin Mouse Monoclonal Antibody (MSN/493).

Click image to see more details

Western blot analysis of Moesin using anti-Moesin antibody (M00766).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human THP-1 whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human Raji whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Moesin antigen affinity purified monoclonal antibody (Catalog # M00766) at 1 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Moesin at approximately 68 kDa. The expected band size for Moesin is at 68 kDa.

Click image to see more details

Formalin-fixed, paraffin-embedded human Placenta stained with Moesin Mouse Monoclonal Antibody (MSN/493).

Click image to see more details

Immunofluorescent staining of paraformaldehyde-fixed HeLa cells. Moesin Mouse Monoclonal Antibody (MSN/493) followed by goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red)

Click image to see more details

Flow Cytometric Analysis of paraformaldehyde-fixed K562 cells. Moesin Mouse Monoclonal Antibody (MSN/493) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red)

Click image to see more details

SDS-PAGE Analysis Purified Moesin Mouse Monoclonal Antibody (MSN/493). Confirmation of Integrity and Purity of Antibody

Gene/Protein Information For MSN (Source: Uniprot.org, NCBI)

Alternative Names

Membrane-organizing extension spike protein; moesin MSN HEL70, IMD50 moesin moesin|epididymis luminal protein 70|membrane-organizing extension spike protein

For more info on MSN, check out the MSN Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for MSN: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the MSN Infographic

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis