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Product Info Summary
| SKU: | A09268-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-IL1F10 Antibody Picoband®
View all IL-38/IL-1F10 Antibodies
SKU/Catalog Number
A09268-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-IL1F10 Antibody Picoband® catalog # A09268-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IL1F10 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09268-1)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human IL1F10 recombinant protein (Position: M1-W152).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A09268-1 is reactive to IL1F10 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
17 kDa
Calculated molecular weight
128790 MW
Background of IL-38/IL-1F10
Interleukin-1 family member 10 is a protein that in humans is encoded by the IL1F10 gene. The protein encoded by this gene is a member of the interleukin 1 cytokine family. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. This cytokine is thought to participate in a network of interleukin 1 family members to regulate adapted and innate immune responses.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09268-1 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
ELISA (Cap), 1-5μg/ml
Positive Control
WB: human U87MG whole cell, human placenta tissue
IHC: human tonsil tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of IL1F10 using anti-IL1F10 antibody (A09268-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human U87MG whole cell lysates,
Lane 2: human placenta tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL1F10 antigen affinity purified polyclonal antibody (Catalog # A09268-1) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL1F10 at approximately 17KD. The expected band size for IL1F10 is at 17KD.
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Figure 2. IHC analysis of IL1F10 using anti-IL1F10 antibody (A09268-1).
IL1F10 was detected in paraffin-embedded section of human tonsil tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-IL1F10 Antibody (A09268-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For IL1F10 (Source: Uniprot.org, NCBI)
Gene Name
IL1F10
Full Name
Interleukin-1 family member 10
Weight
128790 MW
Superfamily
IL-1 family
Alternative Names
Interleukin-1 family member 10; IL-1F10; Family of interleukin 1-theta; FIL1 theta; Interleukin-1 HY2; IL-1HY2; Interleukin-1 theta; IL-1 theta; Interleukin-38; IL-38; IL1F10; FIL1T, IL1HY2, IL38; FKSG75, UNQ6119/PRO20041; IL1F10 FIL1-theta, FKSG75, IL-1HY2, IL-38, IL1-theta, IL1HY2 interleukin 1 family member 10 interleukin-1 family member 10|FIL1 theta|IL-1 theta|IL-1F10 (canonical form IL-1F10a)|family of interleukin 1-theta|interleukin 1 family member 10 (theta)|interleukin-1 HY2|interleukin-1 receptor antagonist FKSG75|interleukin-1 receptor antagonist-like FIL1 theta|interleukin-38
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IL1F10, check out the IL1F10 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IL1F10: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IL1F10 Antibody Picoband® (A09268-1)
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