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Product Info Summary
| SKU: | A30410 |
|---|---|
| Size: | 100uL |
| Reactive Species: | Virus |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-MMTV env Antibody
SKU/Catalog Number
A30410
Size
100uL
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-MMTV env Antibody (Catalog # A30410). Tested in WB applications. This antibody reacts with Virus.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-MMTV env Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30410)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
Antiserum
Immunogen
MMTV was prepared from whole rabbit serum produced by repeated immunizations with a full length sequence for mouse mammary tumor virus surface protein tagged with His.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A30410 is reactive to ENV in Virus
Reconstitution
Calculated molecular weight
11798 MW
Background of ENV
This antibody is suitable for Cancer, Enzyme, and Viral research. Mouse mammary tumor virus surface protein binds the virus to the receptor of the host cells triggering transmembrane protein refolding. This activates the fusion peptide and the structure coils and repeats positioning the fusion peptide near the C-terminus region. Inturn increasing close proximity and then fusion of viral and cell membranes. Anti-MMTV is ideal for researchers interested in Cancer, Viral, and Enzyme research.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30410 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:10,000-1:20,000
WB: 1:1000-1:2000
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of env using anti-env antibody (A30410).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-env antigen affinity purified polyclonal antibody (Catalog # A30410) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for env.
Protein Target Info & Infographic
Gene/Protein Information For ENV (Source: Uniprot.org, NCBI)
Gene Name
ENV
Full Name
Weight
11798 MW
Alternative Names
MMTV, mouse mammary tumor virus surface protein, Envelope glycoprotein gp70, Surface protein, Glycoprotein 52, Transmembrane protein, Glycoprotein 36, Mouse mammary tumor virus (strain GR) (MMTV)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ENV, check out the ENV Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ENV: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MMTV env Antibody (A30410)
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Customer Q&As
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7 Customer Q&As for Anti-MMTV env Antibody
Question
Does A30410 anti-MMTV antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-10-04
Answer
You can see on the product datasheet, A30410 anti-MMTV antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-10-04
Question
Is this A30410 anti-MMTV antibody reactive to the isotypes of env?
Verified Customer
Verified customer
Asked: 2019-07-29
Answer
The immunogen of A30410 anti-MMTV antibody is MMTV was prepared from whole rabbit serum produced by repeated immunizations with a full length sequence for mouse mammary tumor virus surface protein tagged with His. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-07-29
Question
We are currently using anti-MMTV antibody A30410 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says mouse. Is it true that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-15
Answer
The anti-MMTV antibody (A30410) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-15
Question
I see that the anti-MMTV antibody A30410 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-01-09
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-01-09
Question
Do you have a BSA free version of anti-MMTV antibody A30410 available?
Verified Customer
Verified customer
Asked: 2018-05-22
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-MMTV antibody A30410 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-05-22
Question
Is a blocking peptide available for product anti-MMTV antibody (A30410)?
P. Jones
Verified customer
Asked: 2017-01-09
Answer
We do provide the blocking peptide for product anti-MMTV antibody (A30410). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-01-09
Question
Can you help my question with product A30410, anti-MMTV antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
J. Jones
Verified customer
Asked: 2014-09-22
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A30410 anti-MMTV antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2014-09-22
