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Product Info Summary
| SKU: | A03966 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Neurosecretory protein VGF VGF Antibody
SKU/Catalog Number
A03966
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Neurosecretory protein VGF VGF Antibody (Catalog # A03966). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
VGF antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Neurosecretory protein VGF VGF Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03966)
Host
Rabbit
Contents
VGF Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
VGF antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of the human VGF. The immunogen is located within the last 50 amino acids of VGF.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least four isoforms of RABEX5 are known to exist; this antibody will recognize all four. RABEX5 antibody is predicted to not cross-react with other LRRFIP family members.
Reactive Species
A03966 is reactive to VGF in Human, Mouse, Rat
Applications
A03966 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
67258 MW
Background of VGF
VGF was initially identified as a rapidly regulated gene product in nerve growth factor-treated PC12 cells. This protein is synthesized in neurons in the central and peripheral nervous system as well as in the pituitary, adrenal medulla, endocrine cells of the stomach, and pancreatic beta cells. VGF is thought to be involved in organism energy balance and regulation of homeostasis as mice lacking this gene show deficits in these areas. More recent results suggest that VGF is upregulated by brain-derived neurotrophic factor (BDNF) and can stimulate the proliferation of hippocampal progenitor cells and produce antidepressant-like behavioral effects, suggesting that this pathway may be a suitable target for therapeutic treatments.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
VGF antibody can be used for detection of VGF by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of VGF expression in human brain tissue lysate with VGF antibody at (A) 1 and (B) 2 μg/ml.
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Immunofluorescence of VGF in Human Brain cells with VGF antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For VGF (Source: Uniprot.org, NCBI)
Gene Name
VGF
Full Name
Neurosecretory protein VGF
Weight
67258 MW
Alternative Names
neuro-endocrine specific protein VGF; neurosecretory protein VGF; VGF nerve growth factor inducible; VGF VGF SCG7, SgVII VGF nerve growth factor inducible neurosecretory protein VGF|neuro-endocrine specific protein VGF
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on VGF, check out the VGF Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for VGF: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Neurosecretory protein VGF VGF Antibody (A03966)
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