Anti-Neurosecretory protein VGF VGF Antibody Picoband®

Product Name

Anti-Neurosecretory protein VGF VGF Antibody Picoband®

View all VGF Antibodies

SKU/Catalog Number

PA1984

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Neurosecretory protein VGF VGF Antibody catalog # PA1984. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Neurosecretory protein VGF VGF Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1984)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human VGF, different from the related rat and mouse sequences by one amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1984 is reactive to VGF in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

450 kDa

Calculated molecular weight

67258 MW

Background of VGF

VGF (VGF, Nerve Growth Factor-Inducible), is a protein and neuropeptide that may play a role in regulating energy homeostasis, metabolism. Canu et al. (1997) noted that rat Vgf encodes a predicted 70-kD polypeptide that shares similarities with the secretogranin/chromogranin family and is found in the secretory granules of subsets of neurons and endocrine cells. By fluorescence in situ hybridization, Canu et al. (1997) assigned the VGF gene to 7q22. Canu et al. (1997) demonstrated that the single-copy human VGF gene spans 6 kb of genomic DNA and contains 2 exons. The entire VGF protein is encoded by exon 2, while exon 1 contains only 5-prime untranslated sequence. The structural organization of the human gene is similar to that described for the rat VGF gene (Salton et al., 1991), and both the translated and the untranslated regions show a high degree of sequence homology to the rat gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PA1984 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Positive Control

WB: Rat Brain Tissue, U87 Cell, U87 Cell, SHG Cell, NEURO Cell,

Validation Images & Assay Conditions

Gene/Protein Information For VGF (Source: Uniprot.org, NCBI)

Alternative Names

Neurosecretory protein VGF;Neuroendocrine regulatory peptide-1;NERP-1;Neuroendocrine regulatory peptide-2;NERP-2;Antimicrobial peptide VGF[554-577];VGF; VGF SCG7, SgVII VGF nerve growth factor inducible neurosecretory protein VGF|neuro-endocrine specific protein VGF

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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