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Product Info Summary
| SKU: | M00420 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-MMP13 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00420
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-MMP13 Rabbit Monoclonal Antibody catalog # M00420. Tested in WB application. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MMP13 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00420)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
IDA-13
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human MMP13
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00420 is reactive to MMP13 in Human, Mouse
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
52 kDa
Calculated molecular weight
53820 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00420 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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Western blot analysis of MMP13 expression in HeLa cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For MMP13 (Source: Uniprot.org, NCBI)
Gene Name
MMP13
Full Name
Collagenase 3
Weight
53820 MW
Superfamily
peptidase M10A family
Alternative Names
Collagenase 3;3.4.24.-;Matrix metalloproteinase-13;MMP-13;MMP13; MMP13 CLG3, MANDP1, MDST, MMP-13 matrix metallopeptidase 13 collagenase 3|matrix metalloproteinase 13 (collagenase 3)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MMP13, check out the MMP13 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MMP13: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MMP13 Rabbit Monoclonal Antibody (M00420)
Hello CJ!
M00420 has been cited in 4 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Chondroprotective effects of ginsenoside Rg1 in human osteoarthritis chondrocytes and a rat model of anterior cruciate ligament transection
Hydrogen sulfide attenuates IL-1%u03B2-induced inflammatory signaling and dysfunction of osteoarthritic chondrocytes
Effects of ultrasound on estradiol level, bone mineral density, bone biomechanics and matrix metalloproteinase-13 expression in ovariectomized rabbits
Huang Gf, Zou J, Shi J, Zhang Dy, Pen Hf, Zhang Q, Gao Y, Wang By. Evid Based Complement Alternat Med. 2014;2014:731395. Doi: 10.1155/2014/731395. Epub 2014 May 27. The Effect Of Electroacupuncture On The Extracellular Matrix Synthesis And Degrada...
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-MMP13 Rabbit Monoclonal Antibody
Question
Please see the WB image, lot number and protocol we used for mammary carcinoma using anti-MMP13 Rabbit Monoclonal antibody M00420. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-03-26
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-26
Question
I see that the anti-MMP13 Rabbit Monoclonal antibody M00420 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-03-26
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-03-26
Question
I would like to test anti-MMP13 Rabbit Monoclonal antibody M00420 on mouse mammary carcinoma for research purposes, then I may be interested in using anti-MMP13 Rabbit Monoclonal antibody M00420 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-02-25
Answer
The products we sell, including anti-MMP13 Rabbit Monoclonal antibody M00420, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-02-25
Question
We are currently using anti-MMP13 Rabbit Monoclonal antibody M00420 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-19
Answer
The anti-MMP13 Rabbit Monoclonal antibody (M00420) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-19
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for mammary carcinoma using anti-MMP13 Rabbit Monoclonal antibody M00420. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-02-10
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-10
Question
I was wanting to use your anti-MMP13 Rabbit Monoclonal antibody for WB for mouse mammary carcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse mammary carcinoma identification?
M. Jackson
Verified customer
Asked: 2020-02-05
Answer
As indicated on the product datasheet, M00420 anti-MMP13 Rabbit Monoclonal antibody has been tested for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse mammary carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-05
Question
Is there a BSA free version of anti-MMP13 Rabbit Monoclonal antibody M00420 available?
Verified Customer
Verified customer
Asked: 2020-01-08
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-MMP13 Rabbit Monoclonal antibody M00420 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-01-08
Question
Our lab were happy with the WB result of your anti-MMP13 Rabbit Monoclonal antibody. However we have observed positive staining in lung extracellular space using this antibody. Is that expected? Could you tell me where is MMP13 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-01-06
Answer
From what I have seen in literature, lung does express MMP13. Generally MMP13 expresses in secreted, extracellular space, extracellular. Regarding which tissues have MMP13 expression, here are a few articles citing expression in various tissues:
Esophagus, and Synovium, Pubmed ID: 14702039
Lung, Pubmed ID: 15489334
Mammary carcinoma, Pubmed ID: 8207000, 9562863
Boster Scientific Support
Answered: 2020-01-06
Question
Is a blocking peptide available for product anti-MMP13 Rabbit Monoclonal antibody (M00420)?
Verified Customer
Verified customer
Asked: 2019-07-10
Answer
We do provide the blocking peptide for product anti-MMP13 Rabbit Monoclonal antibody (M00420). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-07-10
Question
Will anti-MMP13 Rabbit Monoclonal antibody M00420 work for WB with mammary carcinoma?
Verified Customer
Verified customer
Asked: 2019-04-10
Answer
According to the expression profile of mammary carcinoma, MMP13 is highly expressed in mammary carcinoma. So, it is likely that anti-MMP13 Rabbit Monoclonal antibody M00420 will work for WB with mammary carcinoma.
Boster Scientific Support
Answered: 2019-04-10
Question
Is this M00420 anti-MMP13 Rabbit Monoclonal antibody reactive to the isotypes of MMP13?
Verified Customer
Verified customer
Asked: 2018-12-05
Answer
The immunogen of M00420 anti-MMP13 Rabbit Monoclonal antibody is A synthesized peptide derived from human MMP13. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-12-05
Question
Will M00420 anti-MMP13 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-02-26
Answer
You can see on the product datasheet, M00420 anti-MMP13 Rabbit Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-02-26
Question
My question regards using your anti-MMP13 Rabbit Monoclonal antibody for response to amyloid-beta studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2017-12-05
Answer
Thank you for your inquiry. This M00420 anti-MMP13 Rabbit Monoclonal antibody is tested on hela cell lysate. It is guaranteed to work for WB in human, mouse. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2017-12-05
Question
We have been able to see staining in human esophagus synovium. What should we do? Is anti-MMP13 Rabbit Monoclonal antibody supposed to stain esophagus synovium positively?
P. Kulkarni
Verified customer
Asked: 2017-08-18
Answer
Based on literature esophagus synovium does express MMP13. Based on Uniprot.org, MMP13 is expressed in tibia, mammary carcinoma, esophagus synovium, lung, among other tissues. Regarding which tissues have MMP13 expression, here are a few articles citing expression in various tissues:
Esophagus, and Synovium, Pubmed ID: 14702039
Lung, Pubmed ID: 15489334
Mammary carcinoma, Pubmed ID: 8207000, 9562863
Boster Scientific Support
Answered: 2017-08-18
Question
Can you help my question with product M00420, anti-MMP13 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
G. Parker
Verified customer
Asked: 2013-11-21
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00420 anti-MMP13 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2013-11-21
