Anti-MLK3 (phospho-T277/S281) MAP3K11 Antibody

Product Name

Anti-MLK3 (phospho-T277/S281) MAP3K11 Antibody

View all MLK3 Antibodies

SKU/Catalog Number

A04412T277S281

Size

100ul

Form

Liquid

Description

Boster Bio Anti-MLK3 (phospho-T277/S281) MAP3K11 Antibody catalog # A04412T277S281. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MLK3 (phospho-T277/S281) MAP3K11 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04412T277S281)

Host

Rabbit

Contents

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from human MLK3 around the phosphorylation site of S674.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A04412T277S281 is reactive to MAP3K11 in Human

Applications

A04412T277S281 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

92688 MW

Background of MLK3

Angiogenesis inhibitor. Inhibits migration, proliferation and network formation by endothelial cells as well as angiogenesis. This inhibitory effect is selective to endothelial cells as it does not affect the migration of smooth muscle cells or fibroblasts. Does not affect the proliferation of cancer cells in vitro, but inhibits tumor growth and tumor angiogenesis. Acts in an autocrine manner. Inhibits artery neointimal formation and macrophage infiltration. Exhibits heparin-binding activity.

Kikuno R., DNA Res. 6:197-205(1999).
Bechtel S., BMC Genomics 8:399-399(2007).
Heilig R., Nature 421:601-607(2003).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:1000-1:2000

Validation Images & Assay Conditions

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Figure 1. Western blotting validation for Anti-MLK3 (phospho-T277/S281) MAP3K11 Antibody A04412T277S281

Western blot (WB) analysis of p-MLK3 (T277/S281) polyclonal antibody at 1:500 dilution
Lane1:HEK293Twhole cell lysate
Lane2:NIH-3T3 whole cell lysate
Lane3:PC12 whole cell lysate
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration

Gene/Protein Information For MAP3K11 (Source: Uniprot.org, NCBI)

Alternative Names

EC 2.7.11; EC 2.7.11.25; MAP3K11; MEKK11; mitogen-activated protein kinase kinase kinase 11; Mixed lineage kinase 3; MLK3; MLK3MLK-3; PTK1; PTK1protein-tyrosine kinase PTK1; RHOE; SH3 domain-containing proline-rich kinase; SPRK; SPRKMGC17114; Src-homology 3 domain-containing proline-rich kinase MAP3K11 MEKK11, MLK-3, MLK3, PTK1, SPRK mitogen-activated protein kinase kinase kinase 11 mitogen-activated protein kinase kinase kinase 11|SH3 domain-containing proline-rich kinase|mixed lineage kinase 3|protein-tyrosine kinase PTK1|src-homology 3 domain-containing proline-rich kinase

For more info on MAP3K11, check out the MAP3K11 Infographic

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In this infographic, you will see the following information for MAP3K11: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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