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Product Info Summary
| SKU: | A06078 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, WB |
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Product info
Product Name
Anti-Protein SMG7 SMG7 Antibody
SKU/Catalog Number
A06078
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Protein SMG7 SMG7 Antibody (Catalog # A06078). Tested in WB, IF applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Protein SMG7 SMG7 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06078)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
Antiserum
Immunogen
Synthesized peptide derived from internal of human SMG7.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A06078 is reactive to SMG7 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 100 µL
Calculated molecular weight
127282 MW
Background of SMG7
Plays a role in nonsense-mediated mRNA decay. Recruits UPF1 to cytoplasmic mRNA decay bodies. Together with SMG5 is thought to provide a link to the mRNA degradation machinery involving exonucleolytic pathways, and to serve as an adapter for UPF1 to protein phosphatase 2A (PP2A), thereby triggering UPF1 dephosphorylation.
Ohnishi T., Mol. Cell 12:1187-1200(2003).
Nagase T., DNA Res. 3:321-329(1996).
Nakajima D., DNA Res. 9:99-106(2002).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06078 is guaranteed for IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunofluorescence: 1:100~1:500
Validation Images & Assay Conditions
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Western blot analysis of extracts from HeLa cells, Jurkat cells, 293 cells and HUVEC cells, using SMG7 antibody A06078.
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Immunofluorescence analysis of HepG2 cells, using SMG7 antibody A06078.
Protein Target Info & Infographic
Gene/Protein Information For SMG7 (Source: Uniprot.org, NCBI)
Gene Name
SMG7
Full Name
Protein SMG7
Weight
127282 MW
Alternative Names
Protein SMG7;EST1-like protein C;SMG-7 homolog;hSMG-7;SMG7;C1orf16, EST1C, KIAA0250; SMG7 C1orf16, EST1C, SGA56M SMG7 nonsense mediated mRNA decay factor protein SMG7|EST1 telomerase component homolog C|EST1-like protein C|breast cancer-associated SGA-56M|ever shorter telomeres 1C|smg-7 homolog, nonsense mediated mRNA decay factor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SMG7, check out the SMG7 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SMG7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Protein SMG7 SMG7 Antibody (A06078)
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No publications found for A06078
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-Protein SMG7 SMG7 Antibody
Question
Does A06078 anti-SMG7 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-03-17
Answer
You can see on the product datasheet, A06078 anti-SMG7 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-03-17
Question
We are currently using anti-SMG7 antibody A06078 for human tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-14
Answer
The anti-SMG7 antibody (A06078) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-14
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukemic t-cell using anti-SMG7 antibody A06078. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-08-07
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-07
Question
Would anti-SMG7 antibody A06078 work for WB with leukemic t-cell?
Verified Customer
Verified customer
Asked: 2019-05-17
Answer
According to the expression profile of leukemic t-cell, SMG7 is highly expressed in leukemic t-cell. So, it is likely that anti-SMG7 antibody A06078 will work for WB with leukemic t-cell.
Boster Scientific Support
Answered: 2019-05-17
Question
Is this A06078 anti-SMG7 antibody reactive to the isotypes of SMG7?
Verified Customer
Verified customer
Asked: 2017-12-11
Answer
The immunogen of A06078 anti-SMG7 antibody is Synthesized peptide derived from internal of human SMG7. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-12-11
Question
See below the WB image, lot number and protocol we used for leukemic t-cell using anti-SMG7 antibody A06078. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-11-03
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-11-03
Question
Is a blocking peptide available for product anti-SMG7 antibody (A06078)?
S. Thomas
Verified customer
Asked: 2017-05-31
Answer
We do provide the blocking peptide for product anti-SMG7 antibody (A06078). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-05-31
