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Product Info Summary
| SKU: | M06772 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-MLF1 Antibody (C-term)
View all Myeloid leukemia factor 1 Antibodies
SKU/Catalog Number
M06772
Size
80 µl
Description
Boster Bio Anti-MLF1 Antibody (C-term) (Catalog # M06772). Tested in WB application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-MLF1 Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06772)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This MLF1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 216-250 amino acids from the C-terminal region of human MLF1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M06772 is reactive to MLF1 in Human
Reconstitution
Calculated molecular weight
30627 Da
Background of Myeloid leukemia factor 1
Involved in lineage commitment of primary hemopoietic progenitors by restricting erythroid formation and enhancing myeloid formation. Interferes with erythropoietin-induced erythroid terminal differentiation by preventing cells from exiting the cell cycle through suppression of CDKN1B/p27Kip1 levels. Suppresses RFWD2/COP1 activity via CSN3 which activates p53 and induces cell cycle arrest. Binds DNA and affects the expression of a number of genes so may function as a transcription factor in the nucleus.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M06772 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
Validation Images & Assay Conditions
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All lanes : Anti-MLF1 Antibody (C-term) at 1:2000 dilution
Lane 1: A431 whole cell lysate
Lane 2: U-2OS whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 31 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Protein Target Info & Infographic
Gene/Protein Information For MLF1 (Source: Uniprot.org, NCBI)
Gene Name
MLF1
Full Name
Myeloid leukemia factor 1
Weight
30627 Da
Superfamily
MLF family
Alternative Names
Myeloid leukemia factor 1, Myelodysplasia-myeloid leukemia factor 1, MLF1 MLF1 myeloid leukemia factor 1 myeloid leukemia factor 1|myelodysplasia-myeloid leukemia factor 1|testis tissue sperm-binding protein Li 49e
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MLF1, check out the MLF1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MLF1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MLF1 Antibody (C-term) (M06772)
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No publications found for M06772
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