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Product Info Summary
| SKU: | A08036 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-FAKD2 FASTKD2 Antibody
SKU/Catalog Number
A08036
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-FAKD2 FASTKD2 Antibody (Catalog # A08036). Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FAKD2 FASTKD2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08036)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human FAKD2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A08036 is reactive to FASTKD2 in Human
Reconstitution
Calculated molecular weight
81463 MW
Background of FASTKD2
This gene encodes a protein that is localized in the mitochondrial inner compartment and that may play a role in mitochondrial apoptosis. Nonsense mutations have been reported to result in cytochrome c oxidase deficiency.
Hu R.-M., Proc. Natl. Acad. Sci. U.S.A. 97:9543-9548(2000).
Hillier L.W., Nature 434:724-731(2005).
Ota T., Nat. Genet. 36:40-45(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08036 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
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Western blot analysis of extracts from HepG2 cells, COLO cells and HUVEC cells, using FAKD2 antibody A08036.
Protein Target Info & Infographic
Gene/Protein Information For FASTKD2 (Source: Uniprot.org, NCBI)
Gene Name
FASTKD2
Full Name
FAST kinase domain-containing protein 2, mitochondrial
Weight
81463 MW
Superfamily
FAST kinase family
Alternative Names
FAST kinase domain-containing protein 2, mitochondrial;FASTKD2;KIAA0971; FASTKD2 COXPD44, KIAA0971 FAST kinase domains 2 FAST kinase domain-containing protein 2, mitochondrial
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FASTKD2, check out the FASTKD2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FASTKD2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-FAKD2 FASTKD2 Antibody (A08036)
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No publications found for A08036
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-FAKD2 FASTKD2 Antibody
Question
Does anti-FAKD2 antibody A08036 work on bovine WB with adrenal gland?
Verified Customer
Verified customer
Asked: 2019-07-03
Answer
Our lab technicians have not validated anti-FAKD2 antibody A08036 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-FAKD2 antibody A08036 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine adrenal gland in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-03
Question
Will A08036 anti-FAKD2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-06-14
Answer
You can see on the product datasheet, A08036 anti-FAKD2 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-06-14
Question
We are currently using anti-FAKD2 antibody A08036 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2018-02-12
Answer
The anti-FAKD2 antibody (A08036) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-02-12
Question
I have a question about product A08036, anti-FAKD2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
B. Dhar
Verified customer
Asked: 2017-01-30
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A08036 anti-FAKD2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-01-30
