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Product Info Summary
| SKU: | A03266-1 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Mesothelin/Msln Antibody Picoband®
View all Mesothelin Antibodies
SKU/Catalog Number
A03266-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-Mesothelin/Msln Antibody Picoband® catalog # A03266-1. Tested in ELISA, WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Mesothelin/Msln Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03266-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse Mesothelin/Msln recombinant protein (Position: Q44-K497).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03266-1 is reactive to Msln in Mouse
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
69 kDa
Calculated molecular weight
68.986kDa
Background of Mesothelin
Mesothelin, also known as MSLN or MPF, is a protein that in humans is encoded by the MSLN gene. This gene is mapped to 16p13.3. This gene encodes a precursor protein that is cleaved into two products, megakaryocyte potentiating factor and mesothelin. Megakaryocyte potentiation factor functions as a cytokine that can stimulate colony formation in bone marrow megakaryocytes. Mesothelian is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein. This protein is overexpressed in epithelial mesotheliomas, ovarian cancers and in specific squamous cell carcinomas.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03266-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Mouse
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: mouse heart tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Mesothelin/Msln using anti-Mesothelin/Msln antibody (A03266-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse heart tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Mesothelin/Msln antigen affinity purified polyclonal antibody (Catalog # A03266-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Mesothelin/Msln at approximately 69 kDa. The expected band size for Mesothelin/Msln is at 69 kDa.
Protein Target Info & Infographic
Gene/Protein Information For Msln (Source: Uniprot.org, NCBI)
Gene Name
Msln
Full Name
Mesothelin
Weight
68.986kDa
Superfamily
mesothelin family
Alternative Names
Leucine-rich PPR motif-containing protein, mitochondrial; 130 kDa leucine-rich protein; LRP 130; GP130; LRPPRC; LRP130 MSLN MPF, SMRP mesothelin mesothelin|CAK1 |megakaryocyte potentiating factor|pre-pro-megakaryocyte-potentiating factor|soluble MPF mesothelin related protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Msln, check out the Msln Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Msln: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Mesothelin/Msln Antibody Picoband® (A03266-1)
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