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Product Info Summary
| SKU: | M02354 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-MEKK2 MAP3K2 Rabbit Monoclonal Antibody
SKU/Catalog Number
M02354
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-MEKK2 MAP3K2 Rabbit Monoclonal Antibody catalog # M02354. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MEKK2 MAP3K2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02354)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
FHC-13
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human MEKK2
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02354 is reactive to MAP3K2 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
100 kDa
Calculated molecular weight
69741 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02354 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:5000-1:20000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:30
FC 1:50
Positive Control
IHC: human thyroid cancer tissue
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:6K dilution for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded human thyroid cancer, using MEKK2 Antibody.
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All lanes use the Antibody at 1:6K dilution for 1 hour at room temperature.
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Western blot analysis of MEKK2 expression in HeLa cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For MAP3K2 (Source: Uniprot.org, NCBI)
Gene Name
MAP3K2
Full Name
Mitogen-activated protein kinase kinase kinase 2
Weight
69741 MW
Superfamily
protein kinase superfamily
Alternative Names
Mitogen-activated protein kinase kinase kinase 2;2.7.11.25;MAPK/ERK kinase kinase 2;MEK kinase 2;MEKK 2;MAP3K2;MAPKKK2, MEKK2; MAP3K2 MEKK2, MEKK2B mitogen-activated protein kinase kinase kinase 2 mitogen-activated protein kinase kinase kinase 2|MAP/ERK kinase kinase 2|MAPK/ERK kinase kinase 2|MEK kinase 2|MEKK 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MAP3K2, check out the MAP3K2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MAP3K2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MEKK2 MAP3K2 Rabbit Monoclonal Antibody (M02354)
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Customer Q&As
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4 Customer Q&As for Anti-MEKK2 MAP3K2 Rabbit Monoclonal Antibody
Question
My question regarding product M02354, anti-MEKK2 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-08-16
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M02354 anti-MEKK2 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-08-16
Question
Would anti-MEKK2 Rabbit Monoclonal antibody M02354 work for IF with t-cell?
D. Moore
Verified customer
Asked: 2015-08-21
Answer
According to the expression profile of t-cell, MAP3K2 is highly expressed in t-cell. So, it is likely that anti-MEKK2 Rabbit Monoclonal antibody M02354 will work for IF with t-cell.
Boster Scientific Support
Answered: 2015-08-21
Question
I was wanting to use your anti-MEKK2 Rabbit Monoclonal antibody for IF for mouse t-cell on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse t-cell identification?
H. Zhang
Verified customer
Asked: 2014-11-21
Answer
You can see on the product datasheet, M02354 anti-MEKK2 Rabbit Monoclonal antibody has been tested for IP, IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse t-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-11-21
Question
Does M02354 anti-MEKK2 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
C. Krishna
Verified customer
Asked: 2014-02-26
Answer
It shows on the product datasheet, M02354 anti-MEKK2 Rabbit Monoclonal antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2014-02-26
