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Product Info Summary
| SKU: | P03980 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Phospho-MEK5 (Ser311 Thr315) Antibody
SKU/Catalog Number
P03980
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Phospho-MEK5 (Ser311 Thr315) Antibody (Catalog # P03980). Tested in WB applications. This antibody reacts with Rat.
Storage & Handling
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.
Cite This Product
Anti-Phospho-MEK5 (Ser311 Thr315) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P03980)
Host
Rabbit
Contents
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser311/Thr315 of rat MEK5, conjugated to keyhole limpet hemocyanin (KLH). Immunogen species is Rat.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
P03980 is reactive to MAP2K5 in Rat
Reconstitution
Observed Molecular Weight
52 kDa
Calculated molecular weight
50198 MW
Background of MEK5
MEK5 (also known as MKK5) is a dual specificity serine/threonine protein kinase belonging to the MAP kinase kinase family. MEK5 has been shown to specifically activate ERK5 (Zhou et al., 1995) whereas MEK5 itself is regulated by MEKK3 (Chao et al., 1999). An important link between MEK5 and metastatic prostrate cancer has been demonstrated (Mehta et.al., 2003). Dual phosphorylation of Ser-311 and Thr-315 have been implicated in cell proliferation (Cameron et al., 2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P03980 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
Validation Images & Assay Conditions
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Western blot of rat testes lysate showing specific labeling of the ~49 kDa MEK5 protein phosphorylated at Ser311 Thr315 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).
Protein Target Info & Infographic
Gene/Protein Information For MAP2K5 (Source: Uniprot.org, NCBI)
Gene Name
MAP2K5
Full Name
Dual specificity mitogen-activated protein kinase kinase 5
Weight
50198 MW
Superfamily
protein kinase superfamily
Alternative Names
Dual specificity mitogen activated protein kinase kinase 5 antibody, Dual specificity mitogen-activated protein kinase kinase 5 antibody, EC 2.7.12.2 antibody, HsT17454 antibody, MAP kinase kinase 5 antibody, MAP kinase kinase MEK5b antibody, MAP2K5 antibody, MAPK/ERK kinase 5 antibody, MAPKK 5 antibody, MAPKK5 antibody, MEK 5 antibody, mitogen-activated protein kinase kinase 5 antibody, MKK5 antibody, MP2K5_HUMAN antibody, PRKMK5 antibody, Protein kinase mitogen-activated kinase 5 antibody, SAPKK5 antibody, SKK5 antibody MAP2K5 HsT17454, MAPKK5, MEK5, PRKMK5 mitogen-activated protein kinase kinase 5 dual specificity mitogen-activated protein kinase kinase 5|MAP kinase kinase 5|MAP kinase kinase MEK5b|MAPK/ERK kinase 5|MAPKK 5|MEK 5
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MAP2K5, check out the MAP2K5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MAP2K5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-MEK5 (Ser311 Thr315) Antibody (P03980)
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No publications found for P03980
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1 Customer Q&As for Anti-Phospho-MEK5 (Ser311 Thr315) Antibody
Question
We are currently using anti-Phospho-MEK5 (Ser311 Thr315) antibody P03980 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says rat. Is it possible that the antibody can work on monkey tissues as well?
R. Collins
Verified customer
Asked: 2016-02-04
Answer
The anti-Phospho-MEK5 (Ser311 Thr315) antibody (P03980) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-02-04
