Product Info Summary
SKU: | M01725-1 |
---|---|
Size: | 100ul |
Reactive Species: | Human |
Host: | Mouse |
Application: | ELISA, Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-MEK-4 MAP2K4 Monoclonal Antibody
SKU/Catalog Number
M01725-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-MEK-4 MAP2K4 Monoclonal Antibody catalog # M01725-1. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MEK-4 MAP2K4 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01725-1)
Host
Mouse
Contents
Ascitic fluid containing 0.03% sodium azide.
Clonality
Monoclonal
Clone Number
5H4
Isotype
IgG
Immunogen
Purified recombinant fragment of human MEK-4 expressed in E. Coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01725-1 is reactive to MAP2K4 in Human
Applications
M01725-1 is guaranteed for ELISA, Flow Cytometry, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
44288 MW
Background of MKK4/MEK4
Indoleamine 2, 3-dioxygenase1 (IDO1) is a 41-42 kD intracellular enzyme that catabolizes tryptophan into kynurenine. IDO1 modulates levels of the amino acid tryptophan, which is vital for cell growth, but is also involved in the suppression of the immune response. IDO1 effects on immune suppression are due to decreased tryptophan availability and the generation of tryptophan metabolites, resulting in negative effects on T lymphocytes, including proliferation, function and survival. IDO1 may be involved in the suppression of the immune response to tumors, and blocking the IDO1 pathway may be a potential target for immuno and cancer therapy. IDO1 is expressed in a wide variety of tissues and can be upregulated by interferon gamma and other inflammatory cytokines.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:200-1:1000
FC 1:200-1:400
ELISA 1:10000
Validation Images & Assay Conditions
![m01725 1 anti mek 4 map2k4 monoclonal antibody elisa testing 4 m01725 1 anti mek 4 map2k4 monoclonal antibody elisa testing 4](https://www.bosterbio.com/media/catalog/product/m/0/m01725-1-anti-mek-4-map2k4-monoclonal-antibody-elisa-testing-4.jpg)
Click image to see more details
Figure 4. ELISA validation of MAP2K4 using Anti-MEK-4 MAP2K4 Monoclonal Antibody (M01725-1).
ELISA analysis of MEK-4 antibody.![m01725 1 anti mek 4 map2k4 monoclonal antibody flow cytometry testing 3 m01725 1 anti mek 4 map2k4 monoclonal antibody flow cytometry testing 3](https://www.bosterbio.com/media/catalog/product/m/0/m01725-1-anti-mek-4-map2k4-monoclonal-antibody-flow-cytometry-testing-3.jpg)
Click image to see more details
Figure 3. Flow Cytometry validation of MAP2K4 using Anti-MEK-4 MAP2K4 Monoclonal Antibody (M01725-1).
Flow cytometric (FCM) analysis of K562 cells using MEK-4 Monoclonal Antibody (green) and negative control (purple).![m01725 1 anti mek 4 map2k4 monoclonal antibody ihc testing 2 m01725 1 anti mek 4 map2k4 monoclonal antibody ihc testing 2](https://www.bosterbio.com/media/catalog/product/m/0/m01725-1-anti-mek-4-map2k4-monoclonal-antibody-ihc-testing-2.jpg)
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Figure 2. Immunohistochemistry validation of MAP2K4 using Anti-MEK-4 MAP2K4 Monoclonal Antibody (M01725-1).
Immunohistochemistry (IHC) analysis of paraffin-embedded muscle tissues with DAB staining using MEK-4 Monoclonal Antibody.For more protocol information of IHC
![m01725 1 anti mek 4 map2k4 monoclonal antibody wb testing 1 m01725 1 anti mek 4 map2k4 monoclonal antibody wb testing 1](https://www.bosterbio.com/media/catalog/product/m/0/m01725-1-anti-mek-4-map2k4-monoclonal-antibody-wb-testing-1.jpg)
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Figure 1. Western blotting validation for Anti-MEK-4 MAP2K4 Monoclonal Antibody M01725-1
Western Blot (WB) analysis using MEK-4 Monoclonal Antibody against HepG2 (1)Protein Target Info & Infographic
Gene/Protein Information For MAP2K4 (Source: Uniprot.org, NCBI)
Gene Name
MAP2K4
Full Name
Dual specificity mitogen-activated protein kinase kinase 4
Weight
44288 MW
Superfamily
protein kinase superfamily
Alternative Names
c-Jun N-terminal kinase kinase 1; dual specificity mitogen-activated protein kinase kinase 4; JNK activating kinase 1; JNK-activated kinase 1; JNK-activating kinase 1; JNKK; JNKK1; JNKK1MAPK/ERK kinase 4; MAP kinase kinase 4; MAP2K4; MAPKK4; MEK4; MEK4MEK 4; mitogen-activated protein kinase kinase 4; MKK4; MKK4SAPK/ERK kinase 1; PRKMK4; PRKMK4MAPKK 4; SEK1; SERK1; SERK1EC 2.7.12.2 MAP2K4 JNKK, JNKK1, MAPKK4, MEK4, MKK4, PRKMK4, SAPKK-1, SAPKK1, SEK1, SERK1, SKK1 mitogen-activated protein kinase kinase 4 dual specificity mitogen-activated protein kinase kinase 4|JNK-activated kinase 1|JNK-activating kinase 1|MAP kinase kinase 4|MAPK/ERK kinase 4|MAPKK 4|MEK 4|SAPK/ERK kinase 1|c-Jun N-terminal kinase kinase 1|stress-activated protein kinase kinase 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MAP2K4, check out the MAP2K4 Infographic
![MAP2K4 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MAP2K4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MEK-4 MAP2K4 Monoclonal Antibody (M01725-1)
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