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Product Info Summary
| SKU: | A04799-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, ICC |
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Product info
Product Name
Anti-MED14/Crsp2 Antibody
SKU/Catalog Number
A04799-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-MED14/Crsp2 Antibody (Catalog# A04799-1). Tested in IHC-P, IF, ICC, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MED14/Crsp2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04799-1)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human MED14. Immunogen sequence location: 701-750
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A04799-1 is reactive to MED14 in Human, Mouse
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
72 kDa
Calculated molecular weight
160607 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04799-1 is guaranteed for ELISA, IF, IHC-P, ICC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry, 1:100 - 1:300
Immunofluorescence, 1:200 - 1:1000
ELISA, 1:40000
Not yet tested in other applications.
Validation Images & Assay Conditions
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Immunohistochemistry analysis of paraffin-embedded human brain tissue, using MED14 Antibody. The picture on the right is blocked with the synthesized peptide.
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Immunofluorescence analysis of COS7 cells, using MED14 Antibody. The picture on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For MED14 (Source: Uniprot.org, NCBI)
Gene Name
MED14
Full Name
Mediator of RNA polymerase II transcription subunit 14
Weight
160607 MW
Superfamily
Mediator complex subunit 14 family
Alternative Names
MED14;ARC150;CRSP2;CXorf4;DRIP150;EXLM1;RGR1;TRAP170;Mediator of RNA polymerase II transcription subunit 14;Activator-recruited cofactor 150 kDa component;ARC150;Cofactor required for Sp1 transcriptional activation subunit 2;CRS MED14 CRSP150, CRSP2, CSRP, CXorf4, DRIP150, EXLM1, RGR1, TRAP170 mediator complex subunit 14 mediator of RNA polymerase II transcription subunit 14|ARC150|CRSP complex subunit 2|RGR1 homolog|activator-recruited cofactor 150 kDa component|cofactor required for Sp1 transcriptional activation, subunit 2 (150kD)|hRGR1|human homolog of yeast RGR1|thyroid hormone receptor-associated protein complex 170 kDa component|thyroid hormone receptor-associated protein complex component TRAP170|transcriptional co-activator CRSP150|vitamin D receptor-interacting protein complex component DRIP150|vitamin D3 receptor-interacting protein complex 150 kDa component
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MED14, check out the MED14 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MED14: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MED14/Crsp2 Antibody (A04799-1)
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No publications found for A04799-1
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Customer Reviews
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Customer Q&As
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12 Customer Q&As for Anti-MED14/Crsp2 Antibody
Question
See below the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-MED14/Crsp2 antibody A04799-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-04-30
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-30
Question
Is a blocking peptide available for product anti-MED14/Crsp2 antibody (A04799-1)?
Verified Customer
Verified customer
Asked: 2020-01-20
Answer
We do provide the blocking peptide for product anti-MED14/Crsp2 antibody (A04799-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-01-20
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-MED14/Crsp2 antibody A04799-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-12-20
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-20
Question
Will anti-MED14/Crsp2 antibody A04799-1 work for IHC with cervix carcinoma erythroleukemia?
Verified Customer
Verified customer
Asked: 2019-08-26
Answer
According to the expression profile of cervix carcinoma erythroleukemia, MED14 is highly expressed in cervix carcinoma erythroleukemia. So, it is likely that anti-MED14/Crsp2 antibody A04799-1 will work for IHC with cervix carcinoma erythroleukemia.
Boster Scientific Support
Answered: 2019-08-26
Question
I see that the anti-MED14/Crsp2 antibody A04799-1 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-06-27
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-06-27
Question
Is this A04799-1 anti-MED14/Crsp2 antibody reactive to the isotypes of MED14?
Verified Customer
Verified customer
Asked: 2019-06-13
Answer
The immunogen of A04799-1 anti-MED14/Crsp2 antibody is A peptide derived from human MED14. Immunogen sequence location: 701-750. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-06-13
Question
Does A04799-1 anti-MED14/Crsp2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
C. Jackson
Verified customer
Asked: 2019-04-19
Answer
It shows on the product datasheet, A04799-1 anti-MED14/Crsp2 antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-04-19
Question
My question regarding product A04799-1, anti-MED14/Crsp2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-06-18
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04799-1 anti-MED14/Crsp2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-06-18
Question
Will anti-MED14/Crsp2 antibody A04799-1 work on horse IHC with secondary oocyte?
Verified Customer
Verified customer
Asked: 2017-06-30
Answer
Our lab technicians have not validated anti-MED14/Crsp2 antibody A04799-1 on horse. You can run a BLAST between horse and the immunogen sequence of anti-MED14/Crsp2 antibody A04799-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse secondary oocyte in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-06-30
Question
I was wanting to use your anti-MED14/Crsp2 antibody for IHC for mouse cervix carcinoma erythroleukemia on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse cervix carcinoma erythroleukemia identification?
C. Jones
Verified customer
Asked: 2016-09-20
Answer
As indicated on the product datasheet, A04799-1 anti-MED14/Crsp2 antibody has been tested for IF, IHC on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse cervix carcinoma erythroleukemia in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-09-20
Question
Do you have a BSA free version of anti-MED14/Crsp2 antibody A04799-1 available?
S. Mangal
Verified customer
Asked: 2014-02-18
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-MED14/Crsp2 antibody A04799-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2014-02-18
Question
you antibody to test anti-MED14/Crsp2 antibody A04799-1 on mouse cervix carcinoma erythroleukemia for research purposes, then I may be interested in using anti-MED14/Crsp2 antibody A04799-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Z. Baker
Verified customer
Asked: 2013-03-11
Answer
The products we sell, including anti-MED14/Crsp2 antibody A04799-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2013-03-11
