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Product Info Summary
| SKU: | A04545 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC |
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Product info
Product Name
Anti-MED13 Antibody
SKU/Catalog Number
A04545
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-MED13 Antibody (Catalog # A04545). Tested in IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MED13 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04545)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human MED13.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04545 is reactive to MED13 in Human
Reconstitution
Calculated molecular weight
239297 MW
Background of MED13
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
Olsen J.V., Cell 127:635-648(2006).
Beausoleil S.A., Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004).
Naeaer A.M., Nature 398:828-832(1999).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04545 is guaranteed for IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry: 1:50~1:100
Validation Images & Assay Conditions
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Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using MED13 antibody A04545.
Protein Target Info & Infographic
Gene/Protein Information For MED13 (Source: Uniprot.org, NCBI)
Gene Name
MED13
Full Name
Mediator of RNA polymerase II transcription subunit 13
Weight
239297 MW
Superfamily
Mediator complex subunit 13 family
Alternative Names
Mediator of RNA polymerase II transcription subunit 13;Activator-recruited cofactor 250 kDa component;ARC250;Mediator complex subunit 13;Thyroid hormone receptor-associated protein 1;Thyroid hormone receptor-associated protein complex 240 kDa component;Trap240;Vitamin D3 receptor-interacting protein complex component DRIP250;DRIP250;MED13;ARC250, KIAA0593, THRAP1, TRAP240; MED13 ARC250, DRIP250, HSPC221, MRD61, THRAP1, TRAP240 mediator complex subunit 13 mediator of RNA polymerase II transcription subunit 13|activator-recruited cofactor 250 kDa component|mediator of RNA polymerase II transcription, subunit 13 homolog|thyroid hormone receptor-associated protein 1|thyroid hormone receptor-associated protein complex 240 kDa component|thyroid hormone receptor-associated protein complex component TRAP240|thyroid hormone receptor-associated protein, 240 kDa subunit|vitamin D3 receptor-interacting protein complex component DRIP250
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MED13, check out the MED13 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MED13: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MED13 Antibody (A04545)
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No publications found for A04545
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Customer Reviews
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Customer Q&As
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12 Customer Q&As for Anti-MED13 Antibody
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-MED13 antibody A04545. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-03-17
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-17
Question
Will anti-MED13 antibody A04545 work on horse IHC with liver?
Verified Customer
Verified customer
Asked: 2020-03-10
Answer
Our lab technicians have not tested anti-MED13 antibody A04545 on horse. You can run a BLAST between horse and the immunogen sequence of anti-MED13 antibody A04545 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse liver in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-10
Question
Does A04545 anti-MED13 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-11-07
Answer
You can see on the product datasheet, A04545 anti-MED13 antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-11-07
Question
Is this A04545 anti-MED13 antibody reactive to the isotypes of MED13?
Verified Customer
Verified customer
Asked: 2019-10-02
Answer
The immunogen of A04545 anti-MED13 antibody is Synthesized peptide derived from internal of human MED13. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-10-02
Question
Is there a BSA free version of anti-MED13 antibody A04545 available?
Verified Customer
Verified customer
Asked: 2019-07-24
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-MED13 antibody A04545 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-07-24
Question
I was wanting to use to test anti-MED13 antibody A04545 on human cervix carcinoma erythroleukemia for research purposes, then I may be interested in using anti-MED13 antibody A04545 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-05-31
Answer
The products we sell, including anti-MED13 antibody A04545, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-05-31
Question
Does anti-MED13 antibody A04545 work for IHC with cervix carcinoma erythroleukemia?
Verified Customer
Verified customer
Asked: 2018-12-19
Answer
According to the expression profile of cervix carcinoma erythroleukemia, MED13 is highly expressed in cervix carcinoma erythroleukemia. So, it is likely that anti-MED13 antibody A04545 will work for IHC with cervix carcinoma erythroleukemia.
Boster Scientific Support
Answered: 2018-12-19
Question
Is a blocking peptide available for product anti-MED13 antibody (A04545)?
Verified Customer
Verified customer
Asked: 2018-01-31
Answer
We do provide the blocking peptide for product anti-MED13 antibody (A04545). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-01-31
Question
My question regarding product A04545, anti-MED13 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-10-12
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04545 anti-MED13 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-10-12
Question
I see that the anti-MED13 antibody A04545 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-06-27
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-06-27
Question
See below the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-MED13 antibody A04545. Please let me know if you require anything else.
W. Thomas
Verified customer
Asked: 2017-03-15
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-03-15
Question
I was wanting to use your anti-MED13 antibody for IHC for human cervix carcinoma erythroleukemia on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human cervix carcinoma erythroleukemia identification?
S. Zhao
Verified customer
Asked: 2015-01-02
Answer
You can see on the product datasheet, A04545 anti-MED13 antibody has been tested for IHC on human tissues. We have an innovator award program that if you test this antibody and show it works in human cervix carcinoma erythroleukemia in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-01-02
