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- Table of Contents
Facts about Mediator of RNA polymerase II transcription subunit 13.
Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. .
Human | |
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Gene Name: | MED13 |
Uniprot: | Q9UHV7 |
Entrez: | 9969 |
Belongs to: |
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Mediator complex subunit 13 family |
Activator-recruited cofactor 250 kDa component; KIAA0593HSPC221; mediator complex subunit 13ARC250; mediator of RNA polymerase II transcription, subunit 13 homolog; THRAP1mediator of RNA polymerase II transcription subunit 13; thyroid hormone receptor associated protein 1; Thyroid hormone receptor-associated protein 1; Thyroid hormone receptor-associated protein complex 240 kDa component; thyroid hormone receptor-associated protein complex component TRAP240; thyroid hormone receptor-associated protein, 240 kDa subunit; Trap240; TRAP240DRIP250; Vitamin D3 receptor-interacting protein complex co
Mass (kDA):
239.297 kDA
Human | |
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Location: | 17q23.2 |
Sequence: | 17; NC_000017.11 (61942605..62065983, complement) |
Ubiquitous.
Nucleus.
Boster Bio has high-affinity primary antibodies. We'll explain the benefits of using primary antibodies and how to order one. In addition, we'll discuss the cost of purchasing a primary antibody. This article will also cover the cost of purchasing an antibody, and some of the factors you should consider before making the purchase.
As the leader in antibodies for the detection of human cell surface proteins, Boster Bio offers high-affinity primary antibody against the MED13 marker for use in immunohistochemistry, Western Blotting and ELISA assays. These antibodies have a high binding ability and have been successfully used in research for more than 25 years. They are used extensively in clinical trials and are trusted by the research world.
MED13 is a protein which acts as a bridge between transcription machinery, regulatory proteins, and other proteins. Boster Bio has developed antibodies that recognize this protein. The antibodies used in Boster's biological assays are highly specific and have been validated in ELISA, immunohistochemistry, and Western blotting. The company's MED13L antibodies are available in two- and 3-fold concentrations for different purposes.
It is important to choose the right antibodies for your experiment. There are many things to consider, from the type of antibody to the detection method. An indirect detection method might require a secondary antibody in order to detect an epitope in a sample. Furthermore, sample preparation will affect the antibody's availability. Here are some tips for choosing the right antibody to suit your experiment. When buying a primary antibody using the MED13 marker, keep these things in mind.
Secondary antibodies can be useful when selecting the proper antibody for a specific application. These antibodies bind only to a single region of the antigen. Because of this fact secondary antibodies are often called'secondary source'. Secondary antibodies can bind at the same epitope that the primary antibody, resulting in a stronger signal overall. Using MED13 markers to buy a primary antigen can save you a lot time in the laboratory.
Look for colocalization in order to verify that your primary antibody reacts to the antigen. If antibodies colocalize to a fluorescent antibody, they share the same structure and can then be used as a verification of a specific antigen. The same applies to fluorescent proteins which double-label the same protein. Fluorescent proteins should be co-labeled to the antibody, so that they can be used in research. This will ensure your primary antibody will colocalize with the protein you are interested.
The MED13 marker is used to determine the mutational status for a specific gene. 20029-1-AP recognizes MED13Specific in mouse, human, and rat samples. This molecule belongs to the Mediator complex subunit 13 family, and it acts as a scaffold and bridge to the preinitiation complex of RNA polymerase II. It is not capable of cross-reacting with MED13L.
In vivo, MED13 regulates the cardiac metabolism by increasing the use of fuel with alternating nutrient state. The protein is expressed throughout the body, including in the heart. It is also found in many tissues. The MED13 genetic marker is a valuable genetic tool to study MED13's role in cardiac metabolism. This pathway is controlled by its expression in the heart. However, applications of MED13 markers go beyond cardiac research.
PMID: 10198638 by Ito M., et al. Identity between TRAP and SMCC complexes indicates novel pathways for the function of nuclear receptors and diverse mammalian activators.
PMID: 10235266 by Rachez C., et al. Ligand-dependent transcription activation by nuclear receptors requires the DRIP complex.