Anti-MDMX MDM4 Monoclonal Antibody

Product Name

Anti-MDMX MDM4 Monoclonal Antibody

View all MDMX Antibodies

SKU/Catalog Number

M01889

Size

100ul

Form

Liquid

Description

Boster Bio Anti-MDMX MDM4 Monoclonal Antibody catalog # M01889. Tested in ELISA, IF, IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MDMX MDM4 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01889)

Host

Mouse

Contents

Ascitic fluid containing 0.03% sodium azide.

Clonality

Monoclonal

Clone Number

2D10F4

Isotype

IgG

Immunogen

Purified recombinant fragment of human MDMX expressed in E. Coli.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M01889 is reactive to MDM4 in Human

Applications

M01889 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

54864 MW

Background of MDMX

Indoleamine 2, 3-dioxygenase1 (IDO1) is a 41-42 kD intracellular enzyme that catabolizes tryptophan into kynurenine. IDO1 modulates levels of the amino acid tryptophan, which is vital for cell growth, but is also involved in the suppression of the immune response. IDO1 effects on immune suppression are due to decreased tryptophan availability and the generation of tryptophan metabolites, resulting in negative effects on T lymphocytes, including proliferation, function and survival. IDO1 may be involved in the suppression of the immune response to tumors, and blocking the IDO1 pathway may be a potential target for immuno and cancer therapy. IDO1 is expressed in a wide variety of tissues and can be upregulated by interferon gamma and other inflammatory cytokines.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:200-1:1000
IF 1:200-1:1000
ELISA 1:10000

Validation Images & Assay Conditions

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Figure 3. Immunofluorescent staining data of MDM4 using Anti-MDMX MDM4 Monoclonal Antibody (M01889).

Confocal Immunofluorescence (IF) analysis of HeLa (left) and L-02 (right) cells using MDMX Monoclonal Antibody (green). Red: Actin filaments have been labeled with DY-554 phalloidin.

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Figure 2. Immunohistochemistry validation of MDM4 using Anti-MDMX MDM4 Monoclonal Antibody (M01889).

Immunohistochemistry (IHC) analysis of paraffin-embedded human cerebra (left) and lung carcinoma (right) tissues

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Figure 1. Western blotting validation for Anti-MDMX MDM4 Monoclonal Antibody M01889

Western Blot (WB) analysis using MDMX Monoclonal Antibody against HeLa (1)

Gene/Protein Information For MDM4 (Source: Uniprot.org, NCBI)

Alternative Names

DKFZp781B1423; double minute 4 homolog; Double minute 4 protein; double minute 4, human homolog of; p53-binding protein; HDMX; Mdm2-like p53-binding protein; Mdm4 p53 binding protein homolog (mouse); MDM4 Regulator of P53; MDM4; Mdm4, transformed 3T3 cell double minute 4, p53 binding protein (mouse); Mdm4, transformed 3T3 cell double minute 4, p53 binding protein; MDM4-related protein 1; MDMX; MDMXMGC132766; mouse double minute 4, human homolog of; p53-binding protein; MRP1; p53-binding protein Mdm4; protein Mdm4; Protein Mdmx MDM4 BMFS6, HDMX, MDMX, MRP1 MDM4 regulator of p53 protein Mdm4|MDM4 protein variant G|MDM4 protein variant Y|MDM4, p53 regulator|MDM4-related protein 1|Mdm4 p53 binding protein homolog|double minute 4, human homolog of; p53-binding protein|mdm2-like p53-binding protein|protein Mdmx

For more info on MDM4, check out the MDM4 Infographic

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In this infographic, you will see the following information for MDM4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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