Click image to see more details
Product Info Summary
| SKU: | A01860-1 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IHC-P, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-EPHA4 Antibody (Center)
SKU/Catalog Number
A01860-1
Size
80 µl
Description
Boster Bio Anti-EPHA4 Antibody (Center) (Catalog # A01860-1). Tested in IHC-P, WB application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-EPHA4 Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01860-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This EPHA4 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 383-417 amino acids from the Central region of human EPHA4.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A01860-1 is reactive to EPHA4 in Human, Mouse
Reconstitution
Calculated molecular weight
109860 Da
Background of EPHA4
Receptor tyrosine kinase which binds membrane-bound ephrin family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Highly promiscuous, it has the unique property among Eph receptors to bind and to be physiologically activated by both GPI-anchored ephrin-A and transmembrane ephrin-B ligands including EFNA1 and EFNB3. Upon activation by ephrin ligands, modulates cell morphology and integrin-dependent cell adhesion through regulation of the Rac, Rap and Rho GTPases activity. Plays an important role in the development of the nervous system controlling different steps of axonal guidance including the establishment of the corticospinal projections. May also control the segregation of motor and sensory axons during neuromuscular circuit development. Beside its role in axonal guidance plays a role in synaptic plasticity. Activated by EFNA1 phosphorylates CDK5 at 'Tyr-15' which in turn phosphorylates NGEF regulating RHOA and dendritic spine morphogenesis. In the nervous system, plays also a role in repair after injury preventing axonal regeneration and in angiogenesis playing a role in central nervous system vascular formation. Additionally, its promiscuity makes it available to participate in a variety of cell-cell signaling regulating for instance the development of the thymic epithelium.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01860-1 is guaranteed for IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
IHC-P: 1:25
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of lysates from Hela, MCF-7, SW480, mouse NIH/3T3 cell line, mouse brain tissue lysate (from left to right), using EPHA4 Antibody (Center). A01860-1 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded H. testis section using EPHA4 Antibody (Center). A01860-1 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Protein Target Info & Infographic
Gene/Protein Information For EPHA4 (Source: Uniprot.org, NCBI)
Gene Name
EPHA4
Full Name
Ephrin type-A receptor 4
Weight
109860 Da
Superfamily
protein kinase superfamily
Alternative Names
Ephrin type-A receptor 4, EPH-like kinase 8, EK8, hEK8, Tyrosine-protein kinase TYRO1, Tyrosine-protein kinase receptor SEK, EPHA4, HEK8, SEK, TYRO1 EPHA4 EK8, HEK8, SEK, TYRO1 EPH receptor A4 ephrin type-A receptor 4|EPH-like kinase 8|TYRO1 protein tyrosine kinase|receptor protein-tyrosine kinase HEK8|tyrosine-protein kinase TYRO1|tyrosine-protein kinase receptor SEK
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on EPHA4, check out the EPHA4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EPHA4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-EPHA4 Antibody (Center) (A01860-1)
Hello CJ!
No publications found for A01860-1
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-EPHA4 Antibody (Center)?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-EPHA4 Antibody (Center)
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
