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Product Info Summary
| SKU: | M02107 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Mouse |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-MD-2 LY96 Monoclonal Antibody [9F1B1]
SKU/Catalog Number
M02107
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-MD-2 LY96 Monoclonal Antibody [9F1B1] (Catalog # M02107). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
MD-2 monoclonal antibody can be stored at -20°C, stable for one year.
Cite This Product
Anti-MD-2 LY96 Monoclonal Antibody [9F1B1] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02107)
Host
Mouse
Contents
MD-2 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
Clone: 9F1B1
Isotype
IgG1
Immunogen
Mouse monoclonal MD-2 antibody was raised against a recombinant protein corresponding to amino acids 2 to 160 of human MD-2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
beta-Actin antibody is human, mouse, rat, rabbit, chicken, zebrafish and drosophila reactive.
Reactive Species
M02107 is reactive to LY96 in Human, Mouse
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
18546 MW
Background of MD-2
MD-2 is a member of the Toll/interleukin-1 receptor (TIR) family, a group of proteins that include the Toll-like receptors (TLRs). TLRs are signaling molecules that recognize different pathogen-associated molecular patterns (PAMPs) and serve as an important link between the innate and adaptive immune responses. TLR4, the major signaling receptor for lipopolysaccharide (LPS), requires the binding of MD-2 to its extracellular region for maximal response to LPS. The specificity of this response is determined by the species of MD-2; e.g., human MD-2 transfected into mouse cells can cause mouse TLR4 to react to LPS analogs that are normally antagonistic to human but not mouse TLR4.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02107 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
MD-2 antibody can be used for detection of MD-2 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of (A) 25 and (B) 125 ng of MD-2 recombinant protein with MD-2 antibody at 1 μg/mL.
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Immunohistochemistry of MD-2 in human spleen with MD-2 antibody at 2.5 μg/mL.
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Immunofluorescence of MD2 in human spleen tissue with MD2 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For LY96 (Source: Uniprot.org, NCBI)
Gene Name
LY96
Full Name
Lymphocyte antigen 96
Weight
18546 MW
Alternative Names
MD2, MD-2, ly-96, ESOP-1 Ly96|ESO, ESOP-1, MD-2, MD2|lymphocyte 96|lymphocyte 96|ly-96|myeloid differentiation factor-2|myeloid differentiation protein-2|protein MD-2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LY96, check out the LY96 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LY96: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MD-2 LY96 Monoclonal Antibody [9F1B1] (M02107)
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