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Product Info Summary
| SKU: | A05510 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rat |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-MCT4 SLC16A3 Antibody
SKU/Catalog Number
A05510
Size
100μl
Form
Liquid
Description
Boster Bio Anti-MCT4 SLC16A3 Antibody catalog # A05510. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MCT4 SLC16A3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05510)
Host
Rat
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human MOT4. AA range:233-282
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A05510 is reactive to SLC16A3 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
19 kDa
Calculated molecular weight
49469 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05510 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
ELISA 1:40000
Validation Images & Assay Conditions
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Western blot analysis of various cells using MCT4 Polyclonal Antibody diluted at 1:500
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Western blot analysis of PC-3 cells using MCT4 Polyclonal Antibody diluted at 1:500
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Western blot analysis of lysates from LOVO cells, using MOT4 Antibody. The lane on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For SLC16A3 (Source: Uniprot.org, NCBI)
Gene Name
SLC16A3
Full Name
Monocarboxylate transporter 4
Weight
49469 MW
Superfamily
major facilitator superfamily
Alternative Names
Monocarboxylate transporter 4;MCT 4;Solute carrier family 16 member 3;SLC16A3;MCT4; SLC16A3 MCT 3, MCT 4, MCT-3, MCT-4, MCT3, MCT4 solute carrier family 16 member 3 monocarboxylate transporter 4|monocarboxylate transporter 3|solute carrier family 16 (monocarboxylate transporter), member 3|solute carrier family 16 (monocarboxylic acid transporters), member 3|solute carrier family 16, member 3 (monocarboxylic acid transporter 4)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SLC16A3, check out the SLC16A3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SLC16A3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MCT4 SLC16A3 Antibody (A05510)
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Customer Q&As
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7 Customer Q&As for Anti-MCT4 SLC16A3 Antibody
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for pigmented layer of retina using anti-MCT4 antibody A05510. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-11-15
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-15
Question
Does anti-MCT4 antibody A05510 work on bovine WB with cerebellum?
Verified Customer
Verified customer
Asked: 2019-10-29
Answer
Our lab technicians have not tested anti-MCT4 antibody A05510 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-MCT4 antibody A05510 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine cerebellum in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-29
Question
Is there a BSA free version of anti-MCT4 antibody A05510 available?
Verified Customer
Verified customer
Asked: 2019-08-22
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-MCT4 antibody A05510 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-08-22
Question
Please see the WB image, lot number and protocol we used for pigmented layer of retina using anti-MCT4 antibody A05510. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-05-13
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-13
Question
We are currently using anti-MCT4 antibody A05510 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2018-06-26
Answer
The anti-MCT4 antibody (A05510) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-06-26
Question
I was wanting to use your anti-MCT4 antibody for WB for rat pigmented layer of retina on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat pigmented layer of retina identification?
A. Dhar
Verified customer
Asked: 2018-05-04
Answer
As indicated on the product datasheet, A05510 anti-MCT4 antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat pigmented layer of retina in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-05-04
Question
My question regarding product A05510, anti-MCT4 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-01-16
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A05510 anti-MCT4 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-01-16
