Key=store_id
Key=entity_id, value=47537
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Key=sku, value=EK1670
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Key=created_at, value=2018-08-13 08:23:05
Key=updated_at, value=2024-09-26 00:58:26
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Key=status, value=Enabled
Key=name, value=Human ITIH4 ELISA Kit PicoKine®
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Key=price, value=750.0000
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Key=meta_title, value=Human ITIH4 ELISA Kit Price PicoKine® | BosterBio
Key=meta_description, value=Human ITIH4 ELISA Kit PicoKine® (96 Tests). Quantitate Human ITIH4 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate ).. Sensitivity: 10pg/ml.
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Key=url_key, value=human-itih4-picokine-trade-elisa-kit-ek1670-boster
Key=url_path, value=human-itih4-picokine-trade-elisa-kit-ek1670-boster
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Key=image_label, value=Human ITIH4 PicoKine ELISA Kit standard curve
Key=small_image_label, value=Human ITIH4 PicoKine ELISA Kit standard curve
Key=thumbnail_label, value=Human ITIH4 PicoKine ELISA Kit standard curve
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Key=size, value=96 wells/kit, with removable strips.
Key=gene_full_name, value=inter-alpha-trypsin inhibitor heavy chain family member 4
Key=uniprot_id, value=Q14624
Key=assay_range, value=31.2 pg/ml - 2,000 pg/ml
Key=sensitivity, value=<10 pg/ml
Key=applications, value=ELISA
Key=reactivity, value=Human
Key=cross_reactivity, value=There is no detectable cross-reactivity with other relevant proteins.
Key=principle, value=A monoclonal antibody from mouse|a biotinylated detection polyclonal antibody from goat
Key=anticoagulant, value=heparin or EDTA
Key=assay_procedure_step_1, value=2,000 pg/ml, 1,000 pg/ml, 500 pg/ml, 250 pg/ml, 125 pg/ml, 62.5 pg/ml, 31.25 pg/ml
Key=tmb_incubation_time, value=15-25min
Key=predicted_reactivity
Key=source_company, value=Boster EK1670
Key=product_category, value=Picokine ELISA Kits
Key=research_category, value=Cardiovascular, Immunology, Innate Immunity, Macrophage/Inflammation, Serum Proteins
Key=rating_value, value=100
Key=review_count, value=4
Key=phospho_site
Key=mp_exclude_sitemap, value=Yes
Key=cj_related_products, value=Acute-phase Reaction:PB10081|EKC1730|A03499-4^Neoplasms, Glandular And Epithelial:A06612|A02236-Dyl550|A02236-3
Key=description, value=Human ITIH4 ELISA Kit PicoKine® (96 Tests). Quantitate Human ITIH4 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate ). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Key=short_description, value=Human ITIH4 ELISA Kit PicoKine® (96 Tests). Quantitate Human ITIH4 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate ). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Key=background, value=Inter-alpha-trypsin inhibitor heavy chain H4 is a protein that in humans is encoded by the ITIH4 gene. This gene is mapped to 3p21.1. The protein encoded by this gene is secreted into the blood, where it is cleaved by plasma kallikrein into two smaller forms. Expression of this gene has been detected only in liver, and it seems to be upregulated during surgical trauma. This gene is part of a cluster of similar genes on chromosome 3. Two transcript variants encoding different isoforms have been found for this gene.
Key=gene_name, value=ITIH4
Key=synonyms, value=Inter-alpha-trypsin inhibitor heavy chain H4; ITI heavy chain H4; ITI-HC4; Inter-alpha-inhibitor heavy chain 4; Inter-alpha-trypsin inhibitor family heavy chain-related protein; IHRP; Plasma kallikrein sensitive glycoprotein 120; Gp120; PK-120; ITIH4; IHR
Key=sample_type, value=cell culture supernatants, serum and plasma (heparin, EDTA, citrate ).
Key=kit_components, value=2 ng/tube
Key=componentsneeded, value=

• Microplate reader in standard size.
• Automated plate washer.
• Incubator.
• Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
• Clean tubes and Eppendorf tubes.
• Washing buffer (neutral PBS or TBS).
• Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
• Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

Key=intra_inter_assay_cv, value=

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	48	216	746	58	243	852
Standard deviation	1.97	9.72	42.5	3.6	17.7	64.75
CV (%)	4.1%	4.5%	5.7%	6.2%	7.3%	7.6%

Key=application_details, value=Dilution ratio of 1:1000000, concentration in serum and plasma is around 170 ug/ml.
Key=subcellular_localization, value=Secreted
Key=tissue_specificity, value=Liver specific.
Key=protein_function, value=Type II acute-phase protein (APP) involved in inflammatory responses to trauma. May also play a role in liver development or regeneration.
Key=sample_data, value=

Concentration (pg/ml)	0	31.25	62.5	125	250	500	1000	2000
O.D.	0.015	0.047	0.079	0.148	0.309	0.646	1.322	2.029

Key=immunogen, value=Expression system for standard: NS0; Immunogen sequence: E29-L930
Key=storage, value=Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Key=reproducibility, value=

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	48	49	52	45	48	2.50	5.2%
Sample 2	216	216	209	205	211	4.71	2.2%
Sample 3	746	856	880	835	829	50.63	6.1%

*number of samples for each test n=16.
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