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Product Info Summary
| SKU: | EK1670 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA, citrate ). |
Product info
Product Name
Human ITIH4 ELISA Kit PicoKine®
SKU/Catalog Number
EK1670
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human ITIH4 ELISA Kit PicoKine® (96 Tests). Quantitate Human ITIH4 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate ). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human ITIH4 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1670)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: E29-L930
Sensitivity
<10 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1670 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1670 is reactive to ITIH4 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate ).
Application Guarantee
EK1670 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of ITIH4
Inter-alpha-trypsin inhibitor heavy chain H4 is a protein that in humans is encoded by the ITIH4 gene. This gene is mapped to 3p21.1. The protein encoded by this gene is secreted into the blood, where it is cleaved by plasma kallikrein into two smaller forms. Expression of this gene has been detected only in liver, and it seems to be upregulated during surgical trauma. This gene is part of a cluster of similar genes on chromosome 3. Two transcript variants encoding different isoforms have been found for this gene.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1670-CAP | Anti-Human ITIH4 Pre-coated 96-well strip microplate | 1 |
| EK1670-ST | Human ITIH4 Standard | 2 vials, 2 ng/tube |
| EK1670-DA | Human ITIH4 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.25 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.015 | 0.047 | 0.079 | 0.148 | 0.309 | 0.646 | 1.322 | 2.029 |
Data Example Images
Click image to see more details
Human ITIH4 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect ITIH4, Dilution ratio of 1:1000000, concentration in serum and plasma is around 170 ug/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human ITIH4 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 48 | 216 | 746 | 58 | 243 | 852 |
| Standard deviation | 1.97 | 9.72 | 42.5 | 3.6 | 17.7 | 64.75 |
| CV (%) | 4.1% | 4.5% | 5.7% | 6.2% | 7.3% | 7.6% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of ITIH4 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 48 | 49 | 52 | 45 | 48 | 2.50 | 5.2% |
| Sample 2 | 216 | 216 | 209 | 205 | 211 | 4.71 | 2.2% |
| Sample 3 | 746 | 856 | 880 | 835 | 829 | 50.63 | 6.1% |
Protein Target Info & Infographic
Gene/Protein Information For ITIH4 (Source: Uniprot.Org, NCBI)
Gene Name
ITIH4
Full Name
Inter-alpha-trypsin inhibitor heavy chain H4
Weight
103.357kDa
Superfamily
ITIH family
Alternative Names
Inter-alpha-trypsin inhibitor heavy chain H4; ITI heavy chain H4; ITI-HC4; Inter-alpha-inhibitor heavy chain 4; Inter-alpha-trypsin inhibitor family heavy chain-related protein; IHRP; Plasma kallikrein sensitive glycoprotein 120; Gp120; PK-120; ITIH4; IHR ITIH4 GP120, H4P, IHRP, ITI-HC4, ITIHL1, PK-120, PK120 inter-alpha-trypsin inhibitor heavy chain 4 inter-alpha-trypsin inhibitor heavy chain H4|ITI heavy chain H4|inter-alpha (globulin) inhibitor H4 (plasma Kallikrein-sensitive glycoprotein)|inter-alpha-inhibitor heavy chain 4|inter-alpha-trypsin inhibitor family heavy chain-related protein|inter-alpha-trypsin inhibitor heavy chain family member 4|inter-alpha-trypsin inhibitor, heavy chain-like, 1|plasma kallikrein-sensitive glycoprotein 120
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on ITIH4, check out the ITIH4 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for ITIH4: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human ITIH4 ELISA Kit PicoKine® (EK1670)
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Customer Reviews
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Customer Q&As
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8 Customer Q&As for Human ITIH4 ELISA Kit PicoKine®
Question
Does EK1670 contain native human biological materials?
Verified customer
Asked: 2021-11-10
Answer
The Human ITIH4 ELISA Kit PicoKine® (EK1670) doesn't contain native human biological materials. doesn't contain native human biological materials.
Boster Scientific Support
Answered: 2021-11-11
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification ITIH4 using the EK1670 Human ITIH4 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2020-11-12
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to limit protein degradation.
Boster Scientific Support
Answered: 2020-11-12
Question
Q: how to analyze ELISA data? I have obtained ITIH4 level in serum.
R. Zhou
Verified customer
Asked: 2019-10-09
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2019-10-09
Question
Q: What is the optimal O.D. value for ITIH4 ELISA kit? I used your ITIH4 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain ITIH4 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2019-04-12
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the example mentioned, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this ITIH4 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of ITIH4 that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2019-04-12
Question
Q: if the enzyme conjugated ITIH4 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the ITIH4 antigen?
D. Johnson
Verified customer
Asked: 2019-02-28
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2019-02-28
Question
Q: can I use citrate plasma as samples in Human ITIH4 Picokine® ELISA Kit (Catalog # EK1670)?
Verified Customer
Verified customer
Asked: 2018-10-05
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of ITIH4 causing disruption of its protein structure. ITIH4 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the ITIH4 ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2018-10-05
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-04-28
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2018-04-28
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2015-06-16
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2015-06-16
