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Product Info Summary
| SKU: | PA1711 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-MCM2 Antibody Picoband®
SKU/Catalog Number
PA1711
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MCM2 Antibody catalog # PA1711. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MCM2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1711)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of mouse MCM2, different from the related rat sequence by one amino acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1711 is reactive to Mcm2 in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
102 kDa
Calculated molecular weight
102078 MW
Background of MCM2
MCM2 (MINICHROMOSOME MAINTENANCE, S. CEREVISIAE, HOMOLOG OF, 2),also known as MITOTIN, CDCL1 or BM28, is a human nuclear protein that plays an important role in 2 crucial steps of the cell cycle, namely, onset of DNA replication and cell division. And it is similar to members of the family of early S-phase proteins. The MCM2 gene is mapped to 3q21.3. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre-RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. In the G0 stage, the MCM2 and MCM5 proteins were much less abundant than the MCM7 and MCM3 proteins, which suggests that the MCM proteins are not present in stoichiometric amounts and that only a proportion of these molecules actively participate in cell cycle regulation as part of MCM complexes.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1711 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, By Heat
Immunocytochemistry , 0.5-1μg/ml, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5μg/ml, Mouse
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Mouse
Positive Control
WB: Rat Testis Tissue, Rat Kidney Tissue, Mouse Intestine Tissue, Mouse Testis Tissue, Mouse Ovary Tissue, Mouse Kidney Tissue
IHC: Rat Intestinal Lymphocyte Tissue, Mouse Intestine Tissue
ICC/IF: HEPA1-6 cell
ICC: HEPA Cell, NIH3T3 Cell
FCM: Neuro-2a cell
Validation Images & Assay Conditions
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Anti-MCM2 antibody, PA1711, IHC(P)
IHC(P): Rat Intestinal Lymphocyte Tissue
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Anti-MCM2 antibody, PA1711, IHC(P)
IHC(P): Mouse Intestine Tissue
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Anti-MCM2 antibody, PA1711, Western blotting
All lanes: Anti MCM2 (PA1711) at 0.5ug/ml
Lane 1: Rat Testis Tissue Lysate at 50ug
Lane 2: Rat Kidney Tissue Lysate at 50ug
Lane 3: Mouse Intestine Tissue Lysate at 50ug
Lane 4: Mouse Testis Tissue Lysate at 50ug
Lane 5: Mouse Ovary Tissue Lysate at 50ug
Lane 6: Mouse Kidney Tissue Lysate at 50ug
Predicted bind size: 102KD
Observed bind size: 102KD
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Anti-MCM2 antibody, PA1711, ICC
ICC: HEPA Cell
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Anti-MCM2 antibody, PA1711, ICC
ICC: NIH3T3 Cell
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Figure 6. IF analysis of MCM2 using anti-MCM2 antibody (PA1711).
MCM2 was detected in immunocytochemical section of HEPA1-6 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-MCM2 Antibody (PA1711) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Figure 7. Flow Cytometry analysis of Neuro-2a cells using anti-MCM2 antibody (PA1711).
Overlay histogram showing Neuro-2a cells stained with PA1711 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MCM2Antibody (PA1711, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For Mcm2 (Source: Uniprot.org, NCBI)
Gene Name
Mcm2
Full Name
DNA replication licensing factor MCM2
Weight
102078 MW
Superfamily
MCM family
Alternative Names
DNA replication licensing factor MCM2;3.6.4.12;Minichromosome maintenance protein 2 homolog;Nuclear protein BM28;Mcm2;Bm28, Cdcl1, Kiaa0030, Mcmd2; MCM2 BM28, CCNL1, CDCL1, D3S3194, DFNA70, MITOTIN, cdc19 minichromosome maintenance complex component 2 DNA replication licensing factor MCM2|cell devision cycle-like 1|cyclin-like 1|minichromosome maintenance deficient 2 (mitotin)|minichromosome maintenance protein 2 homolog|nuclear protein BM28
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Mcm2, check out the Mcm2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Mcm2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MCM2 Antibody Picoband® (PA1711)
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Customer Q&As
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17 Customer Q&As for Anti-MCM2 Antibody Picoband®
Question
We are currently using anti-MCM2 antibody PA1711 for rat tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says mouse, rat. Is it possible that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-04
Answer
The anti-MCM2 antibody (PA1711) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-04
Question
Would PA1711 anti-MCM2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-02-21
Answer
As indicated on the product datasheet, PA1711 anti-MCM2 antibody as been validated on ICC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-02-21
Question
We have seen staining in mouse placenta uterus. Do you have any suggestions? Is anti-MCM2 antibody supposed to stain placenta uterus positively?
T. Roberts
Verified customer
Asked: 2019-11-21
Answer
According to literature placenta uterus does express MCM2. According to Uniprot.org, MCM2 is expressed in oocyte, colon carcinoma, bone marrow, brain, lung, lymph, muscle, placenta uterus, cervix carcinoma, prostate cancer, embryonic kidney, t-cell, leukemic t-cell, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have MCM2 expression, here are a few articles citing expression in various tissues:
Bone marrow, Pubmed ID: 7584026
Brain, Lung, Lymph, Muscle, Placenta, and Uterus, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 8258304, 16964243, 17081983, 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Colon carcinoma, Pubmed ID: 8175912
Embryonic kidney, Pubmed ID: 17525332
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Prostate cancer, Pubmed ID: 17487921
T-cell, Pubmed ID: 19367720
Boster Scientific Support
Answered: 2019-11-21
Question
I see that the anti-MCM2 antibody PA1711 works with ICC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-11-13
Answer
You can find protocols for ICC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-11-13
Question
We want to test anti-MCM2 antibody PA1711 on mouse brain for research purposes, then I may be interested in using anti-MCM2 antibody PA1711 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-10-23
Answer
The products we sell, including anti-MCM2 antibody PA1711, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-10-23
Question
Will anti-MCM2 antibody PA1711 work for ICC with brain?
Verified Customer
Verified customer
Asked: 2019-10-14
Answer
According to the expression profile of brain, MCM2 is highly expressed in brain. So, it is likely that anti-MCM2 antibody PA1711 will work for ICC with brain.
Boster Scientific Support
Answered: 2019-10-14
Question
Please see the WB image, lot number and protocol we used for brain using anti-MCM2 antibody PA1711. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-09-10
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-10
Question
Is a blocking peptide available for product anti-MCM2 antibody (PA1711)?
C. Jones
Verified customer
Asked: 2019-07-08
Answer
We do provide the blocking peptide for product anti-MCM2 antibody (PA1711). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-07-08
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-MCM2 antibody PA1711. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-06-26
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-26
Question
Our team were satisfied with the WB result of your anti-MCM2 antibody. However we have observed positive staining in prostate cancer nucleus. using this antibody. Is that expected? Could you tell me where is MCM2 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-06-12
Answer
From what I have seen in literature, prostate cancer does express MCM2. Generally MCM2 expresses in nucleus. Regarding which tissues have MCM2 expression, here are a few articles citing expression in various tissues:
Bone marrow, Pubmed ID: 7584026
Brain, Lung, Lymph, Muscle, Placenta, and Uterus, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 8258304, 16964243, 17081983, 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Colon carcinoma, Pubmed ID: 8175912
Embryonic kidney, Pubmed ID: 17525332
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Prostate cancer, Pubmed ID: 17487921
T-cell, Pubmed ID: 19367720
Boster Scientific Support
Answered: 2019-06-12
Question
Our lab used your anti-MCM2 antibody for IHC on bone marrow in the past. I am using mouse, and We are going to use the antibody for WB next. My question regards examining bone marrow as well as placenta uterus in our next experiment. Do you have any suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2019-05-16
Answer
I looked at the website and datasheets of our anti-MCM2 antibody and it appears that PA1711 has been validated on mouse in both IHC and WB. Thus PA1711 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-05-16
Question
Is this PA1711 anti-MCM2 antibody reactive to the isotypes of MCM2?
Verified Customer
Verified customer
Asked: 2018-11-07
Answer
The immunogen of PA1711 anti-MCM2 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of mouse MCM2(884-904aa DLFKFNKFSRDLKRKLILQQF), different from the related rat sequence by one amino acid. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-11-07
Question
Do you have a BSA free version of anti-MCM2 antibody PA1711 available?
Verified Customer
Verified customer
Asked: 2018-06-18
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-MCM2 antibody PA1711 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-06-18
Question
I was wanting to use using your anti-MCM2 antibody for r-mmu-69052; switching of origins to a post-replicative state studies. Has this antibody been tested with western blotting on intestinal lymphocyte tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2017-05-22
Answer
Thanks for your inquiry. This PA1711 anti-MCM2 antibody is validated on rat kidney tissue, intestinal lymphocyte tissue, tissue lysate, mouse ovary tissue, testis tissue. It is guaranteed to work for IHC, ICC, WB in mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2017-05-22
Question
My question regarding product PA1711, anti-MCM2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
T. Bhatt
Verified customer
Asked: 2017-01-16
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1711 anti-MCM2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-01-16
Question
Does anti-MCM2 antibody PA1711 work on goat IHC with lung?
M. Banerjee
Verified customer
Asked: 2015-11-04
Answer
Our lab technicians have not validated anti-MCM2 antibody PA1711 on goat. You can run a BLAST between goat and the immunogen sequence of anti-MCM2 antibody PA1711 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat lung in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-11-04
Question
I was wanting to use your anti-MCM2 antibody for ICC for mouse brain on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse brain identification?
J. Dhar
Verified customer
Asked: 2013-04-26
Answer
As indicated on the product datasheet, PA1711 anti-MCM2 antibody has been validated for IHC, ICC, WB on mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-04-26
