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Product Info Summary
| SKU: | M00374 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-MCM2 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00374
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-MCM2 Rabbit Monoclonal Antibody catalog # M00374. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MCM2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00374)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
BCA-13
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human MCM2
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00374 is reactive to MCM2 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
31-45 kDa
Calculated molecular weight
101896 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00374 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:5000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Positive Control
IHC: mouse colon tissue
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
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Western blot analysis of MCM2 expression in HeLa cell lysate.
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All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded mouse colon, using MCM2 Antibody.
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All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
Protein Target Info & Infographic
Gene/Protein Information For MCM2 (Source: Uniprot.org, NCBI)
Gene Name
MCM2
Full Name
DNA replication licensing factor MCM2
Weight
101896 MW
Superfamily
MCM family
Alternative Names
DNA replication licensing factor MCM2;3.6.4.12;Minichromosome maintenance protein 2 homolog;Nuclear protein BM28;MCM2;BM28, CCNL1, CDCL1, KIAA0030; MCM2 BM28, CCNL1, CDCL1, D3S3194, DFNA70, MITOTIN, cdc19 minichromosome maintenance complex component 2 DNA replication licensing factor MCM2|cell devision cycle-like 1|cyclin-like 1|minichromosome maintenance deficient 2 (mitotin)|minichromosome maintenance protein 2 homolog|nuclear protein BM28
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MCM2, check out the MCM2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MCM2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MCM2 Rabbit Monoclonal Antibody (M00374)
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No publications found for M00374
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-MCM2 Rabbit Monoclonal Antibody
Question
My question regarding product M00374, anti-MCM2 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-05-06
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00374 anti-MCM2 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-05-06
Question
We are interested in using your anti-MCM2 Rabbit Monoclonal antibody for deafness studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-02-24
Answer
Thanks for your inquiry. This M00374 anti-MCM2 Rabbit Monoclonal antibody is validated on hela cell lysate. It is guaranteed to work for IF, IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-02-24
Question
We are currently using anti-MCM2 Rabbit Monoclonal antibody M00374 for rat tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-23
Answer
The anti-MCM2 Rabbit Monoclonal antibody (M00374) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-23
Question
We bought anti-MCM2 Rabbit Monoclonal antibody for IF on liver a few years ago. I am using mouse, and We are going to use the antibody for IHC next. We want examining liver as well as cervix carcinoma in our next experiment. Could give a recommendation on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2019-09-25
Answer
I looked at the website and datasheets of our anti-MCM2 Rabbit Monoclonal antibody and it appears that M00374 has been tested on mouse in both IF and IHC. Thus M00374 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-09-25
Question
My question regards to test anti-MCM2 Rabbit Monoclonal antibody M00374 on human leukemic t-cell for research purposes, then I may be interested in using anti-MCM2 Rabbit Monoclonal antibody M00374 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-07-26
Answer
The products we sell, including anti-MCM2 Rabbit Monoclonal antibody M00374, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-07-26
Question
Is a blocking peptide available for product anti-MCM2 Rabbit Monoclonal antibody (M00374)?
Verified Customer
Verified customer
Asked: 2019-07-16
Answer
We do provide the blocking peptide for product anti-MCM2 Rabbit Monoclonal antibody (M00374). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-07-16
Question
Our team were content with the WB result of your anti-MCM2 Rabbit Monoclonal antibody. However we have seen positive staining in leukemic t-cell nucleus. using this antibody. Is that expected? Could you tell me where is MCM2 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-02-08
Answer
From literature, leukemic t-cell does express MCM2. Generally MCM2 expresses in nucleus. Regarding which tissues have MCM2 expression, here are a few articles citing expression in various tissues:
Bone marrow, Pubmed ID: 7584026
Brain, Lung, Lymph, Muscle, Placenta, and Uterus, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 8258304, 16964243, 17081983, 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Colon carcinoma, Pubmed ID: 8175912
Embryonic kidney, Pubmed ID: 17525332
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Prostate cancer, Pubmed ID: 17487921
T-cell, Pubmed ID: 19367720
Boster Scientific Support
Answered: 2018-02-08
Question
I was wanting to use your anti-MCM2 Rabbit Monoclonal antibody for IF for human leukemic t-cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human leukemic t-cell identification?
M. Williams
Verified customer
Asked: 2017-10-25
Answer
It shows on the product datasheet, M00374 anti-MCM2 Rabbit Monoclonal antibody has been validated for IF, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human leukemic t-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-10-25
Question
Do you have a BSA free version of anti-MCM2 Rabbit Monoclonal antibody M00374 available?
Verified Customer
Verified customer
Asked: 2017-06-30
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-MCM2 Rabbit Monoclonal antibody M00374 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-06-30
Question
I see that the anti-MCM2 Rabbit Monoclonal antibody M00374 works with IF, what is the protocol used to produce the result images on the product page?
M. Huang
Verified customer
Asked: 2017-03-31
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-03-31
Question
Will M00374 anti-MCM2 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
R. Gonzalez
Verified customer
Asked: 2016-04-06
Answer
As indicated on the product datasheet, M00374 anti-MCM2 Rabbit Monoclonal antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2016-04-06
Question
We have been able to see staining in human bone marrow. Any tips? Is anti-MCM2 Rabbit Monoclonal antibody supposed to stain bone marrow positively?
L. Baker
Verified customer
Asked: 2016-01-13
Answer
From what I have seen in literature bone marrow does express MCM2. From what I have seen in Uniprot.org, MCM2 is expressed in oocyte, colon carcinoma, bone marrow, brain, lung, lymph, muscle, placenta uterus, cervix carcinoma, prostate cancer, embryonic kidney, t-cell, leukemic t-cell, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have MCM2 expression, here are a few articles citing expression in various tissues:
Bone marrow, Pubmed ID: 7584026
Brain, Lung, Lymph, Muscle, Placenta, and Uterus, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 8258304, 16964243, 17081983, 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Colon carcinoma, Pubmed ID: 8175912
Embryonic kidney, Pubmed ID: 17525332
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Prostate cancer, Pubmed ID: 17487921
T-cell, Pubmed ID: 19367720
Boster Scientific Support
Answered: 2016-01-13
Question
See attached the WB image, lot number and protocol we used for leukemic t-cell using anti-MCM2 Rabbit Monoclonal antibody M00374. Please let me know if you require anything else.
A. Williams
Verified customer
Asked: 2015-05-04
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-05-04
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukemic t-cell using anti-MCM2 Rabbit Monoclonal antibody M00374. Let me know if you need anything else.
A. Carter
Verified customer
Asked: 2014-02-05
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-02-05
Question
Is this M00374 anti-MCM2 Rabbit Monoclonal antibody reactive to the isotypes of MCM2?
Z. Mangal
Verified customer
Asked: 2013-09-20
Answer
The immunogen of M00374 anti-MCM2 Rabbit Monoclonal antibody is A synthesized peptide derived from human MCM2. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2013-09-20
Question
Would anti-MCM2 Rabbit Monoclonal antibody M00374 work for IF with leukemic t-cell?
S. Rodriguez
Verified customer
Asked: 2013-01-15
Answer
According to the expression profile of leukemic t-cell, MCM2 is highly expressed in leukemic t-cell. So, it is likely that anti-MCM2 Rabbit Monoclonal antibody M00374 will work for IF with leukemic t-cell.
Boster Scientific Support
Answered: 2013-01-15
