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Product Info Summary
| SKU: | A06515-1 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC-P, WB |
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Product info
Product Name
Anti-MCCC2 Antibody (Center)
SKU/Catalog Number
A06515-1
Size
80 µl
Description
Boster Bio Anti-MCCC2 Antibody (Center) (Catalog # A06515-1). Tested in WB, IHC-P, Flow Cytometry application(s). This antibody reacts with Human, Rat.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-MCCC2 Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06515-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This MCCC2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 163-189 amino acids from the Central region of human MCCC2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A06515-1 is reactive to MCCC2 in Human, Rat
Reconstitution
Calculated molecular weight
61333 Da
Background of MCCC2
MCCC2 is the small subunit of 3-methylcrotonyl-CoA carboxylase. This enzyme functions as a heterodimer and catalyzes the carboxylation of 3-methylcrotonyl-CoA to form 3-methylglutaconyl-CoA.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06515-1 is guaranteed for Flow Cytometry, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
IHC-P: 1:10-1:50
FC: 1:10-1:50
Validation Images & Assay Conditions
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MCCC2 Antibody (Center) western blot analysis in NCI-H292 cell line and rat liver tissue lysates (35ug/lane).This demonstrates the MCCC2 antibody detected the MCCC2 protein (arrow).
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Formalin-fixed and paraffin-embedded human hepatocarcinoma with MCCC2 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Flow cytometric analysis of ATDC5 cells using MCCC2 Antibody (Center) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Protein Target Info & Infographic
Gene/Protein Information For MCCC2 (Source: Uniprot.org, NCBI)
Gene Name
MCCC2
Full Name
Methylcrotonoyl-CoA carboxylase beta chain, mitochondrial
Weight
61333 Da
Superfamily
AccD/PCCB family
Alternative Names
Methylcrotonoyl-CoA carboxylase beta chain, mitochondrial, MCCase subunit beta, 3-methylcrotonyl-CoA carboxylase 2, 3-methylcrotonyl-CoA carboxylase non-biotin-containing subunit, 3-methylcrotonyl-CoA:carbon dioxide ligase subunit beta, MCCC2, MCCB MCCC2 MCCB methylcrotonoyl-CoA carboxylase 2 methylcrotonoyl-CoA carboxylase beta chain, mitochondrial|3-methylcrotonyl-CoA carboxylase 2|3-methylcrotonyl-CoA carboxylase non-biotin-containing subunit|3-methylcrotonyl-CoA:carbon dioxide ligase subunit beta|MCCase subunit beta|biotin carboxylase|methylcrotonoyl-CoA carboxylase 2 (beta)|methylcrotonoyl-Coenzyme A carboxylase 2 (beta)|non-biotin containing subunit of 3-methylcrotonyl-CoA carboxylase|testicular secretory protein Li 29
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MCCC2, check out the MCCC2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MCCC2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MCCC2 Antibody (Center) (A06515-1)
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