Anti-MBD2 Antibody Picoband®

Product Name

Anti-MBD2 Antibody Picoband®

View all MBD2 Antibodies

SKU/Catalog Number

RP1049

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-MBD2 Antibody catalog # RP1049. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MBD2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1049)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human MBD2 recombinant protein (Position: W159-A411). Human MBD2 shares 98% amino acid (aa) sequence identity with mouse MBD2.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

RP1049 is reactive to MBD2 in Human

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

47 kDa

Calculated molecular weight

43255 MW

Background of MBD2

Methyl-CpG-binding domain protein 2 is a protein that in humans is encoded by the MBD2 gene. It is mapped to 18q21.2. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG-binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. The protein encoded by this gene may function as a mediator of the biological consequences of the methylation signal. It is also reported that this protein functions as a demethylase to activate transcription, as DNA methylation causes gene silencing.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

RP1049 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human

Positive Control

WB: SGC Whole Cell, HELA Whole Cell, JURKAT Whole Cell, K562 Whole Cell

Validation Images & Assay Conditions

Gene/Protein Information For MBD2 (Source: Uniprot.org, NCBI)

Alternative Names

Methyl-CpG-binding domain protein 2;Demethylase;DMTase;Methyl-CpG-binding protein MBD2;MBD2; MBD2 DMTase, NY-CO-41 methyl-CpG binding domain protein 2 methyl-CpG-binding domain protein 2|demethylase

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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