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Product Info Summary
| SKU: | M01746-1 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | IHC-P, WB |
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Product info
Product Name
Anti-MBD2 Antibody
SKU/Catalog Number
M01746-1
Size
50 µl
Description
Boster Bio Anti-MBD2 Antibody (Catalog # M01746-1). Tested in WB, IHC-P application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-MBD2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01746-1)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
1812CT625.9.45
Isotype
IgG1,k
Immunogen
This MBD2 antibody is generated from a mouse immunized with a recombinant protein between 10-228 amino acids from human MBD2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01746-1 is reactive to MBD2 in Human
Applications
M01746-1 is guaranteed for IHC-P, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
43255 Da
Background of MBD2
Binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binds hemimethylated DNA as well. Recruits histone deacetylases and DNA methyltransferases. Acts as transcriptional repressor and plays a role in gene silencing. Functions as a scaffold protein, targeting GATAD2A and GATAD2B to chromatin to promote repression. May enhance the activation of some unmethylated cAMP-responsive promoters.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
IHC-P: 1:25
Validation Images & Assay Conditions
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All lanes : Anti-MBD2 Antibody at 1:2000 dilution
Lane 1: K562 whole cell lysate
Lane 2: MCF-7 whole cell lysate
Lane 3: Jurkat whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 43 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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M01746-1 staining MBD2 in human colon tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Protein Target Info & Infographic
Gene/Protein Information For MBD2 (Source: Uniprot.org, NCBI)
Gene Name
MBD2
Full Name
Methyl-CpG-binding domain protein 2
Weight
43255 Da
Alternative Names
demethylase; DKFZp586O0821; DMTase; methyl-CpG binding domain protein 2; methyl-CpG-binding domain protein 2; Methyl-CpG-binding protein MBD2; NY-CO-41 MBD2 DMTase, NY-CO-41 methyl-CpG binding domain protein 2 methyl-CpG-binding domain protein 2|demethylase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MBD2, check out the MBD2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MBD2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MBD2 Antibody (M01746-1)
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