Product Info Summary
SKU: | P02117 |
---|---|
Size: | 100ug |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, WB |
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Product info
Product Name
Anti-Phospho-MARK2 T595 Antibody
SKU/Catalog Number
P02117
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-Phospho-MARK2 T595 Antibody (Catalog # P02117). Tested in ELISA, WB applications. This antibody reacts with Human.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-Phospho-MARK2 T595 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P02117)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids surrounding T595 of Human MARK2 isoform a. The immunogen peptide was phosphorylated at the residue corresponding to T595.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
P02117 is reactive to MARK2 in Human
Applications
P02117 is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
42 kDa
Calculated molecular weight
87911 MW
Background of MARK2
MARK2 pT595 antibody is suitable for Cancer, Immunology and Nuclear Signaling research. This antibody recognizes a regulatory phosphorylation on a kinase that is critical to regulation of microtubules, cell cycle and immune responses. MARK2 refers to MAP/microtubule affinity-regulating kinase 2 isoform a [Homo sapiens]. EMK (ELKL Motif Kinase) is a small family of ser/thr protein kinases involved in the control of cell polarity, microtubule stability and cancer. Several cDNA clones have been isolated that encode two isoforms of the human ser/thr protein kinase EMK1 called MARK2. These isoforms were characterized by the presence of a 162-bp alternative exon that gives rise to two forms, one containing the exon and the other one lacking it. Both forms were found to be co-expressed in a number of selected cell lines and tissue samples. Human MARK2 was shown to be encoded by a single mRNA that is ubiquitously expressed. This transcription variant includes the alternative exon in the coding region and therefore codes for a longer protein. Multiple splice variants exist for this protein.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:1,000 - 1:3,000
IF Microscopy: User optimized
WB: 1:250 - 1:1,000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of MARK2 expression in wild type Jurkat cells (lane 1) and wild type Jurkat cells stimulated with CD3/CD28 (T-cell receptor stimulation) (lane 2). MARK2 at 83KD was detected using rabbit anti-MARK2 pT595 Antigen Affinity purified polyclonal antibody (Catalog # P02117) at 1:500.
Click image to see more details
Western blot analysis of MARK2 expression in 293T cells untransfected (lane 1), 293T cells transfected with MARK2 and PKC-zeta (lane 2) and 293T cells transfected with MARK2 (lane 3). MARK2 at 83KD was detected using rabbit anti-MARK2 pT595 Antigen Affinity purified polyclonal antibody (Catalog # P02117) at 1:1000.
Protein Target Info & Infographic
Gene/Protein Information For MARK2 (Source: Uniprot.org, NCBI)
Gene Name
MARK2
Full Name
Serine/threonine-protein kinase MARK2
Weight
87911 MW
Superfamily
protein kinase superfamily
Alternative Names
EC 2.7.11; EC 2.7.11.1; ELKL motif kinase 1; ELKL motif kinase; EMK-1; EMK1PAR-1; MAP/microtubule affinity-regulating kinase 2MGC99619; PAR1 homolog; Par1b; Ser/Thr protein kinase PAR-1B; serine/threonine protein kinase EMK; serine/threonine-protein kinase MARK2 MARK2 EMK-1, EMK1, PAR-1, Par-1b, Par1b microtubule affinity regulating kinase 2 serine/threonine-protein kinase MARK2|ELKL motif kinase 1|MAP/microtubule affinity-regulating kinase 2|PAR1 homolog b|Ser/Thr protein kinase PAR-1B|serine/threonine protein kinase EMK|testicular tissue protein Li 117
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MARK2, check out the MARK2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MARK2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-MARK2 T595 Antibody (P02117)
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Customer Q&As
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5 Customer Q&As for Anti-Phospho-MARK2 T595 Antibody
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for platelet using anti-Phospho-MARK2 T595 antibody P02117. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-02-11
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-11
Question
We are currently using anti-Phospho-MARK2 T595 antibody P02117 for human tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-30
Answer
The anti-Phospho-MARK2 T595 antibody (P02117) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-30
Question
See attached the WB image, lot number and protocol we used for platelet using anti-Phospho-MARK2 T595 antibody P02117. Please let me know if you require anything else.
P. Collins
Verified customer
Asked: 2019-05-22
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-22
Question
My question regarding product P02117, anti-Phospho-MARK2 T595 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-06-15
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free P02117 anti-Phospho-MARK2 T595 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-06-15
Question
Is a blocking peptide available for product anti-Phospho-MARK2 T595 antibody (P02117)?
A. Li
Verified customer
Asked: 2017-05-17
Answer
We do provide the blocking peptide for product anti-Phospho-MARK2 T595 antibody (P02117). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-05-17